2005
DOI: 10.1093/nar/gni140
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Transformation of isolated mammalian mitochondria by bacterial conjugation

Abstract: We have developed a method for transferring exogenous DNA molecules into isolated mammalian mitochondria using bacterial conjugation. In general, we accomplish this by (i) inserting an origin of DNA transfer (oriT) sequence into a DNA construct, (ii) transforming the construct into an appropriate Escherichia coli strain and then (iii) introducing the mobilizable DNA into mitochondria through conjugation. We tested this approach by transferring plasmid DNA containing a T7 promoter sequence into mitochondria tha… Show more

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Cited by 104 publications
(36 citation statements)
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“…So far, the only organism whose mitochondrial genome can be manipulated virtually at will is the yeast Saccharomyces cerevisiae (6)(7)(8). Mitochondrial transformation of other organisms is a current challenge that stimulates extensive research, especially concerning mammalian mitochondrial genomes (9).…”
mentioning
confidence: 99%
“…So far, the only organism whose mitochondrial genome can be manipulated virtually at will is the yeast Saccharomyces cerevisiae (6)(7)(8). Mitochondrial transformation of other organisms is a current challenge that stimulates extensive research, especially concerning mammalian mitochondrial genomes (9).…”
mentioning
confidence: 99%
“…Conjugation is already a mechanism for the interkingdom exchange of genetic information. Interkingdom conjugation by bacteria has been demonstrated not only in plants (7,40) but also in yeast (28), in mammalian cells (68), and in mitochondria isolated from mouse cells (69). DNA has been introduced successfully into fungal and plant mitochondria (8,17,63).…”
Section: Discussionmentioning
confidence: 99%
“…How this occurs remains unclear, but increasing evidence supports the possibility of direct nucleic acid passage across the mitochondrial membrane through porin channels (62). Investigators, recognizing that mitochondria have a bacterial origin, have utilized both electroporation and bacterial conjugation to successfully deliver mtDNA, presumably through porin channels as happens in bacteria (24,138). Mitochondria can also acquire genetic material, potentially through a similar mechanism.…”
Section: Methods In Mitochondrial Transfectionmentioning
confidence: 99%