Transformation of organic contamination from wastewater into bioplastics (polyhydroxyalkanoate) by microorganisms

Fra-Vázquez, Andrea; Palmeiro-Sánchez, Tania; Río, Ángeles Val del; Mosquera-Corral, Anuska

doi:10.1016/b978-0-12-816204-0.00018-7

Cited by 4 publications

(1 citation statement)

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“…11,12 Regarding the culture selection stage, optimal performance encompasses an adequate selection of microorganisms with high storage capability and high growth rate, as both contribute to increasing the global PHA volumetric productivity. 13,14 It is well known that a PHA-storing bacterial enrichment can be achieved using alternating feast and famine operation conditions. 2 High values of biomass productivity are usually obtained for higher organic loading rates (OLRs), but increasing the OLR above a certain threshold extends the feast phase to a point where flanking, nonstoring populations overcome their internal metabolic limitations and start to grow.…”

Section: ■ Introductionmentioning

confidence: 99%

Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant

Matos

Cruz

Cardoso

et al. 2021

ACS Sustainable Chem. Eng.

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The full-scale implementation of polyhydroxyalkanoate (PHA) production using mixed microbial cultures (MMCs) has been limited by the low PHA global productivity and overall process yield. This work aimed to demonstrate, at a pilot scale, that by combining different effective operating conditions, it is possible to boost the PHA production performance when using fruit waste as a substrate. The organic loading rate (OLR) and pH of the acidogenic reactor were successfully used as tuning parameters to obtain a high fermentation yield (0.74 gCOD•gCOD −1 ) and a fermentate rich in butyrate, resulting in enhanced PHA production steps. A biomass highly enriched in PHA-storing microorganisms was selected as a result of uncoupling the carbon to the nitrogen feeding. The biomass concentration attained a notable value (7.83 g•L −1 ) as a response to the high OLR (8.7 gCOD•L −1 •d −1 ) imposed. In the PHA accumulation assays, the culture selected at the optimal OLR in the selection reactor achieved a high storage yield (0.98 gCOD•gCOD −1 ), and the continuous feeding strategy led to a maximum PHA content of 80.5% (g-basis). The high global productivity (8.1 g-PHA•L −1 •d −1 ) and overall process yield (0.45 gCOD•gCOD −1 ) are, to the best of the authors' knowledge, the highest values reported for MMC using a real feedstock at pilot scale. These results demonstrate the importance of combining different effective strategies to maximize the process performance, a promising result toward the full-scale implementation of PHA production from wastes and MMC.

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