Transformation of Streptococcus sanguis Challis by plasmid deoxyribonucleic acid from Streptococcus faecalis

LeBlanc, Donald J.; Hassell, F P

doi:10.1128/jb.128.1.347-355.1976

Cited by 119 publications

(43 citation statements)

References 33 publications

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“…The lysate was enriched for plasmid DNA by shearing and NaCl-saturated phenol extraction according to Currier and Nester (4). Covalently closed circular plasmid DNA was separated from nicked plasmid and residual chromosomal DNA by dye buoyant density gradient centrifugation, as previously described (11). The isolated plasmid band, after extraction of ethidium bromide and removal of cesium chloride by dialysis (11), was further purified by a second banding in a dye buoyant density gradient, and dye and cesium chloride were again removed.…”

Section: Methodsmentioning

confidence: 99%

“…After the DNA was precipitated as described above, it was resuspended in 10 mM Tris-hydrochloride-10 mM EDTA-50 mM NaCl (pH 8.0). To 9 ml of DNA in this buffer, 10.61 g of cesium chloride was added and centrifuged to equilibrium in a 50 Ti rotor (Beckman Instruments Inc., Fullerton, Calif.) at 35,000 rpm and 20°C for 64 h. The gradient was fractionated, and aliquots were counted as previously described (11). Fractions containing the DNA were pooled and dialyzed against the buffer.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1

LeBlanc¹,

Lee²

1982

J Bacteriol

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Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugants obtained after mixed incubation of JH1 with the plasmid-free S. faecalis strain JH2-2 revealed the presence of two tetracycline resistance determinants in strain JH1. One determinant mediated constitutive resistance to 40 ,ug of tetracycline per ml and was associated with plasmid pJH1. The second determinant, either on the chromosome of strain JH1 or on an undetectable plasmid, was inducible by tetracycline and enabled the host strain, in the absence of pJH1, to grow in the presence of 80 ,ug of tetracycline per ml. One transconjugant, strain DL172, was resistant to 80 ,g of tetracycline per ml, sensitive to kanamycin, streptomycin, and erythromycin, and hemolytic in the presence, but not in the absence, of tetracycline. A single plasmid, pDL172, from this strain consisted of plasmid pJH2 and a 17.8-kilobase segment of DNA homologous to total cell DNA from strain JH1 but did not contain plasmid pJH1. Whether the addition of heterologous DNA to plasmid pJH2 occurred by translocation of a 17.8-kilobase tetracycline resistance transposon or by classical recombination with pJH2 has not been determined.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1

LeBlanc¹,

Lee²

1982

J Bacteriol

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“…The majority of other streptococal bacteriocin-like agents are of comparatively low molecular weight with relatively broad inhibitory spectra (3,21,22,27,28,31,32). However, unlike the colicins, bacteriocin STHl is probably not plasmid-encoded, as strain DLl does not appear to harbor an extrachromosomal DNA element (18).…”

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confidence: 99%

Bacteriocin production and sensitivity among coaggregating and noncoaggregating oral streptococci

Tompkins

Peavey

Birchmeier

et al. 1997

Oral Microbiology and Immunology

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Twenty-one oral Streptococcus isolates of known interbacterial coaggregation groups were tested against one another (as both producers and indicators) to detect bacteriocin-like inhibitory activity. In agar-based antagonism tests, seven strains produced small inhibitory zones (< or = 3 mm diameter) but in liquid medium, only strain Streptococcus gordonii DL1 (Challis) produced a detectable antibacterial action (bacteriocin STH1). Five strains were sensitive to bacteriocin STH1, but neither the production of nor the sensitivity to any of the antagonistic agents correlated with coaggregation groupings. Four strains (C219, 903, 118 and Wicky) developed stable resistance in response to the bacteriocin, whereas one isolate (strain 34) remained sensitive following repeated bacteriocin exposure. With one exception (strain 903), bacteriocin STH1-sensitive strains were competent for genetic transformation, but not all competent strains were bacteriocin-sensitive. Bacteriocin-resistant derivatives of transformable strains exhibited decreased competence (80-90% reduction) compared with their parent strains.

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“…U12643). Chimeric pMJB8b plasmids (Table 1) containing the custom oligonucleotides were inserted into S. gordonii by transformation (LeBlanc and Hasell 1976). Chromosomal integration was verified by PCR amplification using GFP and gtfG primers on DNA extracted from kanamycin resistant colonies.…”

Section: Methodsmentioning

confidence: 99%

Construction of genetically engineeredStreptococcus gordoniistrains to provide control in QPCR assays for assessing microbiological quality of recreational water

James

Genthner

2008

Journal of Applied Microbiology

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Aims: Quantitative polymerase chain reaction (QPCR) methods for beach monitoring by estimating abundance of Enterococcus spp. in recreational waters use internal, positive controls which address only the amplification of target DNA. In this study two internal, positive controls were developed to control for both amplification and cell lysis in assays measuring abundance of vegetative Gram‐positive bacteria. Methods and Results: Controls were constructed using Streptococcus gordonii DL‐1, a naturally transformable, Gram‐positive bacterium. Unique target sequences were provided by chromosomal insertion of a genetically modified, green fluorescent protein gene fragment. Results suggest that their use for control of lysis and amplification may be of significant value. Conclusions: The use of these controls and the establishment of data quality objectives to determine the tolerable level of decision error should ensure that environmental decisions based on QPCR data are technically and scientifically sound. Significance and Impact of the Study: QPCR measurements related to cell abundance may vary between samples as thick‐walled Gram‐positive bacteria are inherently difficult to lyse and substances present in recreational waters may inhibit amplification. As QPCR methods are considered for beach monitoring, it is essential to demonstrate that the data obtained accurately reflects the abundance of the bacterial indicator.

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Transformation of Streptococcus sanguis Challis by plasmid deoxyribonucleic acid from Streptococcus faecalis

Cited by 119 publications

References 33 publications

Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1

Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1

Bacteriocin production and sensitivity among coaggregating and noncoaggregating oral streptococci

Construction of genetically engineeredStreptococcus gordoniistrains to provide control in QPCR assays for assessing microbiological quality of recreational water

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