Transformation of sundew: pitfalls and promises

Blehová, Alžbeta; Švubová, Renáta; Lukačová, Zuzana; Moravčíková, Jana; Matušíková, Ildikó

doi:10.1007/s11240-014-0635-9

Cited by 8 publications

(11 citation statements)

References 40 publications

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“…To the best of our knowledge, five articles about genetic transformation of carnivorous plants are available (Hirsikorpi et al 2002 ; Królicka et al 2010 ; Blehova et al 2015 ; Miguel et al 2019 ; Oropeza-Aburto et al 2020 ), while only one is about transformation with wild strains of R. rhizogenes bacteria (Królicka et al 2010 ). Hirsikorpi et al ( 2002 ) and Oropeza-Aburto et al ( 2020 ) used Rhizobium tumefaciens (former: Agrobacterium tumefaciens ) as a vector organism, while in the work of Blehova et al ( 2015 ), R. rhizogenes with green fluorescent protein gene served for Drosera rotundifolia L. transformation. Furthermore, Miguel et al ( 2019 ) explored virus-based plant transformation to create transgenic sundew and pitcher plants, for the overproduction of recombinant proteins.…”

Section: Discussionmentioning

confidence: 99%

“…When plant cells are challenged with Rhizobium, the stress-induced defense response appears, based on induction of stress-involved gene expression patterns and up-regulation of enzymatic protein activity in the phenylpropanoid pathway, leading to increased synthesis of secondary metabolites (Franklin et al 2009 ; Tusevski et al 2019 ). Being a rich source of phenolic compounds with strong bactericidal properties, carnivorous plants from the family Droseraceae are hard to transform (Blehova et al 2015 ), while transformation of such organisms gives the possibility to study pathways involved in the synthesis of valuable secondary metabolites and/or overproducing phytochemicals of interest (Gandhi et al 2015 ). Moreover, effective transformation protocols for the Venus flytrap seem to be important tools in the field of physiological, ecological, and evolutionary research (Blehova et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Being a rich source of phenolic compounds with strong bactericidal properties, carnivorous plants from the family Droseraceae are hard to transform (Blehova et al 2015 ), while transformation of such organisms gives the possibility to study pathways involved in the synthesis of valuable secondary metabolites and/or overproducing phytochemicals of interest (Gandhi et al 2015 ). Moreover, effective transformation protocols for the Venus flytrap seem to be important tools in the field of physiological, ecological, and evolutionary research (Blehova et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the implementation of biotechnological tools for the enhancement of secondary metabolite production, like elicitation (Putalun et al 2010 ; Boonsnongcheepa et al 2019 ) or genetic transformation became possible (Królicka et al 2010 ). Nevertheless, increasing valuable phytochemicals with elicitation and obtaining transformed plants is a difficult task because of carnivorous plant biology (Blehova et al 2015 ; Makowski et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Makowski

Królicka

Nowicka

et al. 2021

Appl Microbiol Biotechnol

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The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Makowski

Królicka

Nowicka

et al. 2021

Appl Microbiol Biotechnol

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“…Further, indirect immunolocalization of actin as well as tubulin has indicated important role in prehaustorial cells in C. europaea (Kaštier et al 2015). The techniques used in those studies, however, do not allow to study the dynamic changes in cytoskeleton organization in real-time in living cells, therefore would largely benefit from a use of a vital marker like GFP Blehová et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Transient expression of green fluorescent protein in parasitic dodder as a tool for studying of cytoskeleton

Kaštier¹,

Martinčová²,

Fiala³

et al. 2017

Nova Biotechnologica Et Chimica

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Dodder (Cuscuta) species cause severe agricultural damage in many countries throughout the world. To establish strategies for control of its growth and spreading it is important to study its life cycle and survival strategies. For these efforts genetic modification would represent a powerful tool. Here we report on Agrobacteriummediated transformation of dodder using green fluorescent protein (GFP) fused to actin-binding protein as a vital marker. Since the shoot of germinating C. europaea contains a functional apical meristem and grows quickly comparing to the root-like structure, the shoot apex was used here as explant. The transgene expression was only transient, nevertheless it enabled to detect allocation of actin filaments and studying the cytoskeleton organization in dodder shoot apex. Transient expression of GFP appears to be a suitable method for studying Cuscuta development through cytoskeleton organisation that is presently largely unexplored.

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Molecular characterization and evolution of carnivorous sundew (Drosera rotundifolia L.) class V β-1,3-glucanase

Michalko

Renner

Mészáros

et al. 2016

Planta

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A gene for β-1,3-glucanase was isolated from carnivorous sundew. It is active in leaves and roots, but not in digestive glands. Analyses in transgenic tobacco suggest its function in germination. Ancestral plant β-1,3-glucanases (EC 3.2.1.39) played a role in cell division and cell wall remodelling, but divergent evolution has extended their roles in plant defense against stresses to decomposition of prey in carnivorous plants. As available gene sequences from carnivorous plants are rare, we isolated a glucanase gene from roundleaf sundew (Drosera rotundifolia L.) by a genome walking approach. Computational predictions recognized typical gene features and protein motifs described for other plant β-1,3-glucanases. Phylogenetic reconstructions suggest strong support for evolutionary relatedness to class V β-1,3-glucanases, including homologs that are active in the traps of related carnivorous species. The gene is expressed in sundew vegetative tissues but not in flowers and digestive glands, and encodes for a functional enzyme when expressed in transgenic tobacco. Detailed analyses of the supposed promoter both in silico and in transgenic tobacco suggest that this glucanase plays a role in development. Specific spatiotemporal activity was observed during transgenic seed germination. Later during growth, the sundew promoter was active in marginal and sub-marginal areas of apical true leaf meristems of young tobacco plants. These results suggest that the isolated glucanase gene is regulated endogenously, possibly by auxin. This is the first report on a nuclear gene study from sundew.

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Transformation of sundew: pitfalls and promises

Cited by 8 publications

References 40 publications

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Transient expression of green fluorescent protein in parasitic dodder as a tool for studying of cytoskeleton

Molecular characterization and evolution of carnivorous sundew (Drosera rotundifolia L.) class V β-1,3-glucanase

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