Transformation of sweet potato (Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin

Newell, C. A.; Lowe, J.; Merryweather, A.; Rooke, L.; Hamilton, W. D.

doi:10.1016/0168-9452(95)04109-8

Cited by 81 publications

(41 citation statements)

References 25 publications

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“…In the authors' experiments, the obtained transformation frequencies of about 0.21% indicates the difficulty of Agrobacterium mediated transformation of insecticidal genes into sweet potato. This result is also associated with our previous studies where insecticidal genes cry3ca1 and vip1-2 were used (experimental data unpublished) and other reports [17,18]. To date, several reports on the Agrobacterium-mediated transformation of sweet potato have been published.…”

Section: Biological Test Resultssupporting

confidence: 73%

“…The sweet potato weevil is the major sweet potato insect pest worldwide, however, only three reports on transformed plants with insecticidal genes cowpea trypsin inhibitor, snowdrop lectin genes and cryIII gene have been published thus far [17,18,31]. Also, it is known that Bt is widely used in pest control agents, especially highly activate against lepidopteran pests.…”

Section: Biological Test Resultsmentioning

confidence: 99%

“…However majority of the most efficient and robust Agrobacterium transformation systems succeed in transformation of marker genes such as gfp, gusA, nptII, bar [5,10,[34][35][36][37]. Therefore, genetic transformation of sweet potato remains cumbersome and labor-intensive due to the difficulty in plant regeneration of several recalcitrant cultivars, and the reproducibility of the used protocol [15,17,34,[36][37][38][39] .…”

Section: Biological Test Resultsmentioning

confidence: 99%

“…Since the Agrobacterium mediated transformation system does not involve sophisticated equipment and frequently produces cleaner events (intact integrations and single copy) than other methods [13], it remains to be the more favorable approach. Previous studies on sweet potato transformation via Agrobacterium tumefaciens transformation have been reported including herbicide resistance [14,15], viral resistance [16], insect resistances related to the CryIIIA delta-endotoxin, cowpea trypsin inhibitor and snowdrop lectin [17,18] and enhancing starch accumulation in tubers [19,20]. To date, effective transformation protocols have been developed for only few of sweet potato cultivars, indicating that it is a very recalcitrant species and transformation methods of sweet potato are still genotype dependent.…”

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confidence: 99%

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Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Ngoc¹,

Lan²,

Trang³

et al. 2015

JLS

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Sweet potato [Ipomoea batatas (L.) Lam.] is an important food crop in the world as well as in Vietnam. It is well known as a recalcitrant crop for gene transformation and tissue culture because of its genotype dependent in vitro responses. In present study, Agrobacterium-mediated transformation of cry8Db from Bacillus thuringiensis into KB1 sweet potato variety has been studied. The C58cv strain carrying a pBI121 backbone which contained cry8Db delta-endotoxin gene regulated under 35S CaMV promoter, and the selection marker gene, neomycin phosphotransferase (nptII) gene, was subjected for plant transformation. Callus induced from shoot tips and leaf explants were inoculated and cocultured with A. tumefaciens. The selection occurred during callus producing and plant regenerating steps. A total of 201 transgenic putative plant lines were produced, and 21 transgenic lines were positively confirmed by PCR and finalized by Southern blot. Four putative transgenic lines confirming a single copy of the cry8Db gene were transferred into soil pots in greenhouse. Biological activity evaluation for the insecticidal capacity of these transgenic lines under controlled conditions showed that the level of infestation by sweet potato weevils (Cylas formicarius) in untransformed plants was higher than that of transgenic lines.

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Section: Biological Test Resultssupporting

confidence: 73%

Section: Biological Test Resultsmentioning

confidence: 99%

Section: Biological Test Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Ngoc¹,

Lan²,

Trang³

et al. 2015

JLS

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“…There have been a number of proposals for the in vitro propagation of sweetpotato, including via adventitious shoot induction Dessai et al, 1995;Sihachakr et al, 1997;Santa-Maria et al, 2009) and somatic embryogenesis (Liu and Cantliffe, 1984;Chée et al, 1990;Desamero et al, 1994;Bieniek et al, 1995;Zheng et al, 1996;Dhir et al, 1998;He et al, 2009). Genetic transformation of sweetpotato, using various explants, has been reported, but with low efficiency (Newell et al, 1995;Gama et al, 1996;Morán et al, 1998;Okada et al, 2001;Berberich et al, 2005). Callus mediated transgenic plant regeneration using embryogenic suspension cultures has been reported by Yu et al, (2007), but this procedure may lead to genetic variations.…”

Section: Introductionmentioning

confidence: 99%

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Kumar¹,

Ku²,

Agrawal³

et al. 2013

bta

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In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron (TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants. It appeared that the frequency of protuberance development depended on the concentration of TDZ. However, this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully established in a greenhouse.

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Sweet Potato

Dhir

Singh

2008

Compendium of Transgenic Crop Plants

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The sweet potato, Ipomoea batatas (L.) Lam., is an important root crop supporting millions of people throughout the tropical regions of the world and recognized as world's seventh most important food crop following wheat, rice, maize, potato, barley, and cassava. Sweet potato can produce more edible energy per hectare per day than wheat, rice, or cassava, and possesses potent antioxidant, antidiabetic, and anti‐inflammatory properties while use of starch and other processed goods (e.g., alcohol, etc.) is more localized, and increasingly provides an additional source of cash for poor. Despite the fact that tremendous improvements have been made in sweet potatoes, very little progress has been made in terms of yield when compared to other crops. The major challenges conventional breeding faces are the existence of extensive inherent genetic variability, presence of cross incompatible genes, and low seed set rates. Genetic transformation using tissue culture/biotechnology is an alternative that is looked on to as a promising tool to achieve such goals. Various gene delivery systems have been used to develop transgenic sweet potato concentrating on a specific trait/character, which includes Agrobacterium ‐mediated transformation, electroporation, and biolistic approaches. However, the success of transgenic effort can only be achieved by optimizing a successful regeneration protocol and careful consideration of the likely expected effects of transgenic plants on biodiversity of plants, insects, and humans.

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Transformation of sweet potato (Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin

Cited by 81 publications

References 25 publications

Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Sweet Potato

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