Transformation of the Mycorrhizal Basidiomycetes,<i>Suillus grevillei</i>and<i>S. bovinus</i>, by Particle Bombardment

Sunagawa, Masahide; Murata, Hitoshi; Miyazaki, Yasumasa; Nakamura, Masaya

doi:10.1271/bbb.60328

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…The transformation efficiency for the generation of geneticin-resistant colonies did not differ significantly from that of the hygromicin-resistant colonies, and we confirmed that the setting combination of 1100 psi of helium pressure and a 6 cm target distance was more effective than other settings (data not shown). The settings established in this study were similar to those found for the biolistic transformation of mycelia of the mycorrhizal basidiomycetes Suillus grevillei and Suillus bovinus (Sunagawa et al 2007). …”

Section: Biolistic Variablessupporting

confidence: 73%

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Biolistic transformation of the violet root rot fungus Helicobasidium mompa

et al. 2015

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The violet root rot fungus, Helicobasidium mompa, is agriculturally important, but development of a genetic transformation system for the fungus has been limited. In this study, H. mompa V17 and V664 isolates were biolistically transformed with a plasmid expressing the hygromycin B phosphotransferase (hph) gene. Hygromycin-resistant strains were obtained from both isolates. Various numbers of copies of the hph gene expression cassette were integrated into the H. mompa genome. Most transformants grew stably on selective and nonselective media, suggesting that biolistic transformation is an efficient method to generate mitotically stable H. mompa transformants.

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Section: Biolistic Variablessupporting

confidence: 73%

“…(Sunagawa et al 2007), and Uromyces fabae (Djulic et al 2011). Because this method can deliver DNA directly into mycelia, protoplasts are not required.…”

Section: Introductionmentioning

confidence: 99%

Biolistic transformation of the violet root rot fungus Helicobasidium mompa

et al. 2015

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Cell Factories of Higher Fungi for Useful Metabolite Production

Qin

Xiao

et al. 2015

Bioreactor Engineering Research and Industrial Applications I

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Higher fungi or called as macro-fungi, consisting of the divisions ascomycetes, basidiomycetes, and imperfect fungi, are receiving great interest around the world, because studies of higher fungi help us not only to find new edible and officinal resources but also to understand their complicated biology. In recent decades, a large number of useful substances from higher fungi have been isolated, identified, and characterized, which have important biological functions, such as reducing blood pressure, enhancing immunity, and possessing anti-cancer and anti-HIV and other pharmacological activities. This chapter will review the genetic manipulation tools for higher fungi, omics analysis of higher-fungus cell factories, and production of useful metabolites by higher fungi, including those of terpenoids, heterocyclics, polysaccharides, and polyketides. Trends in future development of cell factories of higher fungi for useful metabolite production will also be analyzed. Graphical Abstract Strategies for improving cell factories of higher fungi for useful metabolite production.

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