Transformation of Theileria parva derived from African buffalo (Syncerus caffer) by tick passage in cattle and its use in infection and treatment immunization

Maritim, A.C.; Young, A. S.; Lesan, A.C.; Ndungu, S.G.; Stagg, D.A.; Ngumi, P.N.

doi:10.1016/0304-4017(92)90043-9

Cited by 18 publications

(8 citation statements)

References 20 publications

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“…Although buffalo-derived T. parva readily infect cattle, they differentiate poorly to the erythrocytic piroplasm stage and therefore are not readily transmitted by ticks to other cattle ( Neitz, 1957 , Schreuder et al, 1977 , Maritim et al, 1992 ). Given the poor differentiation to tick-transmissible stages in cattle, transmission of these parasites to buffalo is also likely to be highly inefficient.…”

Section: Discussionmentioning

confidence: 99%

Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci

Hemmink

Sitt

Pellé

et al. 2018

International Journal for Parasitology

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Section: Discussionmentioning

confidence: 99%

Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci

Hemmink

Sitt

Pellé

et al. 2018

International Journal for Parasitology

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“…Adding to this genetic evidence of separate host-associated populations are several epidemiological clues (reviewed in Morrison et al 2020). These include the fact that tick transmission of buffalo-derived T. parva to other cattle has only been achieved on a few occasions and at low efficiency (Neitz 1957;Maritim et al 1992), and previously described immunological observations that differentiate the two parasites (Radley et al 1979;Radley 1981). The description of new Apicomplexa species based on genetic information alone is controversial (Krief et al 2010;Rayner et al 2011) but not unprecedented (Liu et al 2017), and it has been proposed that species identification based only on DNA characterization would be more efficient, even though inclusion of other data, such as host and geography, when available, is advisable (Cook et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Capture-based enrichment ofTheileria parvaDNA enables full genome assembly of first buffalo-derived strain and reveals exceptional intra-specific genetic diversity

Palmateer

Tretina

Orvis

et al. 2020

Preprint

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Theileria parva is an economically important, intracellular, tick-transmitted parasite of cattle. A live vaccine against the parasite is effective against challenge from cattle-transmissible T. parva but not against genotypes originating from the African Cape buffalo, a major wildlife reservoir, prompting the need to characterize genome-wide variation within and between cattle-and buffalo-associated T. parva populations. Here, we describe a capture-based target enrichment approach that enables, for the first time, de novo assembly of nearly complete T. parva genomes derived from infected host cell lines. This approach has exceptionally high specificity and sensitivity and is successful for both cattle-and buffaloderived T. parva parasites. De novo genome assemblies generated for cattle genotypes differ from the reference by ~54K single nucleotide polymorphisms (SNPs) throughout the 8.31 Mb genome, an average of 6.5 SNPs/kb. We report the first buffalo-derived T. parva genome, which is larger than the genome from the reference, cattle-derived, Muguga strain. The average non-synonymous nucleotide diversity (pN) per gene, between buffalo-derived T. parva and the Muguga strain, was 1.3%. This remarkably high level of genetic divergence is supported by an average FST, genome-wide, of 0.44, reflecting a degree of genetic differentiation between cattle-and buffalo-derived T. parva parasites more commonly seen between, rather than within, species, with clear implications for vaccine development. The DNA capture approach used provides clear advantages over alternative T. parva DNA enrichment methods used previously and enables in-depth comparative genomics in this apicomplexan parasite.

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“…The cattle received 0-5 ml (equivalent to 11 ticks) of the same GUTS subcutaneously in front and above the left parotoid lymph node. A stabilate (ST 199) was made from the same material (Maritim et al 1992).…”

Section: Methodsmentioning

confidence: 99%

“…These stocks represented two different isolations. The stabilates (ST 199; ST3O18) have been described by Maritim et al (1992). In one experiment (Exp.…”

Section: Parasitesmentioning

confidence: 99%

“…Two African buffalo, KB2 and 7014, which were carriers of T. parva, were used as a source of buffaloderived T. parva. These buffalo were housed at the Wildlife Diseases Section, Kenya Agricultural Research Institute, Kabete and their history has been described by Young et al (1977a) and Maritim et al (1992). Buffalo 7668 was born in captivity, was not infected and was used as a source of cells for in vitro infections (Stagg et al 1983).…”

Section: Buffalomentioning

confidence: 99%

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Characterization of Theileria parva which infects waterbuck (Kobus defassa)

et al. 1994

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Theileria-free waterbuck (Kobus defassa) born in captivity were successfully infected with Theileria parva sporozoites derived from ticks infected by feeding on African buffalo (Syncerus caffer). All waterbuck underwent mild infections with the development of sporadic schizont and piroplasm parasitosis when inoculated with sporozoite doses lethal to cattle. A carrier state of T. parva was demonstrated by feeding clean R. appendiculatus nymphs on two of these infected waterbuck. Tick batches from these waterbuck on 2 of 5 occasions transmitted lethal Theileria infections to cattle. In a separate experiment, waterbuck cells were infected and transformed in vitro by T. parva sporozoites derived from buffalo but not by cattle-derived T. parva (Muguga) sporozoites. Waterbuck cells infected in vitro with T. parva isolated from buffalo were inoculated into autologous waterbuck but no infections developed. Theileria parva isolates generated in this study from various sources were characterized using anti-T. parva schizont monoclonal antibodies (MAbs), and it was found that buffalo-derived and waterbuck-passaged isolates had different profiles. Species-specific synthetic oligonucleotide probes, restriction fragment length polymorphism (RFLP) analysis with cloned T. parva DNA probes, and DNA sequence analysis of the p67 sporozoite antigen gene confirmed that the waterbuck-passaged parasite was T. parva. The Tpr repetitive probe hybridization patterns from the waterbuck-passaged parasites were different from the other samples tested. The ribosomal genotype of the waterbuck-passaged T. parva was similar to that of cattle-derived T. parva Muguga. Analyses with both probes and MAbs suggested that a minor parasite population present within the T. parva 7014 buffalo-derived stock had been selected during waterbuck passage.(ABSTRACT TRUNCATED AT 250 WORDS)

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Transformation of Theileria parva derived from African buffalo (Syncerus caffer) by tick passage in cattle and its use in infection and treatment immunization

Cited by 18 publications

References 20 publications

Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci

Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci

Capture-based enrichment ofTheileria parvaDNA enables full genome assembly of first buffalo-derived strain and reveals exceptional intra-specific genetic diversity

Characterization of Theileria parva which infects waterbuck (Kobus defassa)

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