Transformation of tomato cultivar ‘Pusa Ruby’ with bspA gene from Populus tremula for drought tolerance

Roy, R.; Purty, Ram Singh; Agrawal, Veena; Gupta, Shrish C.

doi:10.1007/s11240-005-9000-3

Cited by 38 publications

(26 citation statements)

References 49 publications

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“…Cefotaxime (500 mg/l) was optimized to control the overgrowth of bacteria and also not affect the callus growth and regeneration. These results are in confirmatory with Roy et al (2006); they also used 500 mg/l of cefotaxime to control bacteria in tomato cv. Pusa Ruby for drought tolerance.…”

Section: Different Concentrations Of Cefotaxime In Preselectionsupporting

confidence: 82%

“…The plasmid DNA of a potential canker resistance gene (pC822) from the Xa21 gene family of rice (which provides broad spectrum Xanthomonas resistance in rice) was PEG-mediated cotransformed into protoplasts. Tomato is a model plant and has been transformed with different genes, such as anti-ripening (Picton et al, 1993;Reed et al, 1996), insect (Fischhoff et al, 1987) and herbicide tolerance (Fillatti et al, 1987b), virus resistance (Kim et al, 1994;Raj et al, 2005) and salt tolerance (Gidbert et al, 2000;Jia et al, 2002;Roy et al, 2006). Agrobacterium mediated transformation in tomato has been reported by various researchers (Romero et al, 2001;Rui, et al, 2000;Costa et al, 2000;Saiqun et al, 1999).…”

Section: Regenerationmentioning

confidence: 99%

“…Two types of explants, leaf discs and hypocotyls, were used in the present research. Many workers reported the hypocotyls (Jatoi et al, 1995;Park et al, 2003) and leaf disc (Soniya et al, 2001;Raj et al, 2005;Roy et al, 2006) …”

Section: Factors Affecting Transformation Efficiencymentioning

confidence: 99%

“…These findings are contradictory to Arillaga et al (2000), who reported that the root formation from the transgenic shoots from cotyledons explants occurred kanamycin. Roy et al (2006) used 40 mg/l of hygromycin as a selective marker for drought tolerance in tomato cv. Pusa Ruby.…”

Section: Selection Of Transformantsmentioning

confidence: 99%

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An improved Agrobacterium mediated transformation in tomato using hygromycin as a selective agent

Chaudhry¹,

Hamid²

2010

Afr. J. Biotechnol.

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Bacterial wilt is a devastating disease of tomato crop throughout the world. This disease is very dangerous in hot and humid regions, where it spreads with the irrigation water to whole field within days, which resulted in severe decline in yield. Two varieties of tomato were used for developing bacterial wilt resistance. Riogrande responded more efficiently as compared to Roma. Regeneration frequency of 90.6% was achieved when leaf discs were used and 82.5% when hypocotyls were used as explant for tomato cv. Riogrande. While 65.4% regeneration was achieved from hypocotyls and 72.6% regeneration was obtained when leaf discs were used as explant for cv. Roma. Explants were cocultivated with Agrobacterium strain EHA101 containing a binary vector pTCL5, having hygromycin phosphotransferase (HPT) gene which confers resistance to hygromycin and β-glucuronidase (GUS) gene in addition to Xa21 gene. Hygromycin (25 mg/l) was used as selectable marker while GUS is a reporter gene. Acetosyringone (50 M) enhanced transformation effeciency. Preselection period of 7 days was found to be indispensable for successful transformation of tomato crop. Transformation efficiency of 24% was observed for Riogrande and 8% for Roma. Molecular analysis of transgenic plants produced was carried out for hygromycin resistance gene. Transgenic plants contained the expected band of 670 bp. PCR analysis confirmed the foreign DNA in to the plant genome.

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Section: Different Concentrations Of Cefotaxime In Preselectionsupporting

confidence: 82%

Section: Regenerationmentioning

confidence: 99%

Section: Factors Affecting Transformation Efficiencymentioning

confidence: 99%

Section: Selection Of Transformantsmentioning

confidence: 99%

See 2 more Smart Citations

An improved Agrobacterium mediated transformation in tomato using hygromycin as a selective agent

Chaudhry¹,

Hamid²

2010

Afr. J. Biotechnol.

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“…Arid and semiarid regions of the world are badly afected by water stress, and as result crop production is reduced. Irrigated areas sometimes face drought conditions due to inadequate supply of water and canal closures [23,29]. Drought-tolerant varieties are those, where grain yield is least afected by drought stress, or drought-tolerant crops are those that take up maximum amount of water and lose minimum of water during dry conditions [1][2][3][4][5]30].…”

Section: Drought Stress In Pakistanmentioning

confidence: 99%

Association Mapping of Root Traits for Drought Tolerance in Bread Wheat

Ahmad¹

2017

Wheat Improvement, Management and Utilization

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Bread wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is one of the most important crops, making staple food for more than 40 countries and over 35% of the global population. Drought stress is among the major constraints to wheat production as it afects plant growth, gene expression and yield potential of the crop. Development of elite wheat cultivars with the ability to grow and reproduce in water-limited soils seems to be the most enduring solution of addressing drought stress. A total of 100 lines including well-adapted wheat cultivars were evaluated for important root traits and complemented with 102 PCR-based markers aiming to understand their genetic structure and to identify molecular markers that are closely associated to quantitative trait loci (QTLs) of important root traits. Alleles per locus are counted and polymorphic information content (PIC) values are calculated. Population structure of these lines was analyzed with general linear model (GLM) and mixed linear model (MLM) approaches for identiication of QTLs associated with important root traits. The results indicated the presence of two novel QTLs on the homoeologous group 2 and group 5 of wheat that may be related to drought stress resistance. Our results may facilitate the development of agronomically desirable drought stress-resistant wheat germplasm.

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Tomato

Fatima

Mattoo

Rivera-Domínguez

et al. 2008

Compendium of Transgenic Crop Plants

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Tomato is one of the most consumed and widely grown vegetable crops in the world. It is a popular vegetable/fruit and an important source of vitamins and minerals in human diet. One medium‐sized tomato provides 57% of the recommended daily allowance (RDA) of vitamin C, 25% RDA of vitamin A, and 8% RDA of iron, yet with only 35 calories. Tomatoes are also rich in an antioxidant called lycopene, a carotenoid that has been found to protect cells from oxidants that have been linked to cancer. Tomato is a member of the dicot family Solanaceae , which contains well‐known plant species such as potato, tobacco, eggplant, and pepper. The fruit of tomato is classified as a climacteric fruit, in which onset of ripening is accompanied by an increase in both respiration and ethylene production. Conventional plant breeding has had a significant impact on improving tomato breeding for fruit quality and resistance to important diseases. However, this progress has been confounded by the following: the time‐consuming process of making crosses and backcrosses; difficulty in combating the evolution of new virulent pathogens or breakdown of resistance; saturation of the genetic pools available for improvement; time required, 10 to 15 years, to release a new tomato variety. Besides, traditional breeding methods used may not impart the desired characteristics. Genetic engineering or “recombinant‐DNA technology” is now a highly promising avenue for transplanting genetic characteristics of one species into another. This technology is based on identifying the molecular unit(s) responsible for the desired agronomic trait and then isolating and determining the structure and organization of such a unit or units. The unit can then be engineered to transform and modify any existing variety of a crop plant. This technology provides a continuum between plant breeding‐based varietal development and manipulation of beneficial genes by genetic engineering to develop transgenics. This chapter summarizes some of the major studies conducted thus far to manipulate ripening, fruit texture, flavor, and nutritional quality of tomato fruit using the transgenic approach.

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Transformation of tomato cultivar ‘Pusa Ruby’ with bspA gene from Populus tremula for drought tolerance

Cited by 38 publications

References 49 publications

An improved Agrobacterium mediated transformation in tomato using hygromycin as a selective agent

An improved Agrobacterium mediated transformation in tomato using hygromycin as a selective agent

Association Mapping of Root Traits for Drought Tolerance in Bread Wheat

Tomato

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