Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation

Wirth, Reinhard; Friesenegger, Anita; Fiedler, S.

doi:10.1007/bf00332248

Cited by 117 publications

(63 citation statements)

References 7 publications

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“…Adult mosquitoes were targeted for paratransgenesis method (Lindh et al, 2008). Trans-stadial characters were found among the bacteria in both Gram-negative and gram-positive (Wirth et al, 1989). Load of the malaria parasite in infected mosquitoes by bacteria were reduced the prevalence of the Plasmodium falciparum and P. vivax (Gonzalez-Ceron et al, 2003, Pumpuni et al, 1996.…”

Section: Resultsmentioning

confidence: 99%

Bacterial Flora of the Anopheles fluviatilis S. L., the Vector of Malaria in Southern Iran for Proper Candidate Paraterasgenesis

Soleimani¹,

Kazemi²,

Vatandoost³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultsmentioning

confidence: 99%

Bacterial Flora of the Anopheles fluviatilis S. L., the Vector of Malaria in Southern Iran for Proper Candidate Paraterasgenesis

Soleimani¹,

Kazemi²,

Vatandoost³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…The most striking merit of DRAQ5™ is that it can easily stain gram-positive bacteria, with which transformation of plasmids is normally difficult. Although previous research has shown that diverse grampositive bacteria can be transformed via electroporation, it still remains difficult (Dubnau, 1999;Wirth et al, 1989). Although DRAQ5™ is very useful dye for the fluorescence study, it also has disadvantages, including high toxicity to humans and lack of specificity for target bacteria.…”

Section: Discussionmentioning

confidence: 99%

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

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The bacteria B1-9 that was isolated from the rhizosphere of the green onion could promote growth of pepper, cucumber, tomato, and melon plants. In particular, pepper yield after B1-9 treatment on the seedling was increased about 3 times higher than that of control plants in a field experiment. Partial 16S rDNA sequences revealed that B1-9 belongs to the genus Pantoea ananatis. Pathogenecity tests showed non-pathogenic on kimchi cabbage, carrot, and onion. The functional characterization study demonstrated B1-9's ability to function in phosphate solubilization, sulfur oxidation, nitrogen fixation, and indole-3-acetic acid production. To trace colonization patterns of B1-9 in pepper plant tissues, we used DRAQ5™ fluorescent dye, which stains the DNAs of bacteria and plant cells. A large number of B1-9 cells were found on the surfaces of roots and stems as well as in guard cells. Furthermore, several colonized B1-9 cells resided in inner cortical plant cells. Treatment of rhizosphere regions with strain B1-9 can result in efficient colonization of plants and promote plant growth from the seedling to mature plant stage. In summary, strain B1-9 can be successfully applied in the pepper plantation because of its high colonization capacity in plant tissues, as well as properties that promote efficient plant growth.

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“…Plasmid DNA was prepared with a High Pure Plasmid Isolation Kit (Roche Applied Science). Escherichia coli was transformed by the rubidium chloride method (Wirth et al ., 1989). Mycobacterium smegmatis cells were transformed by electroporation (Gene Pulser; Bio‐Rad) (Parish and Stoker, 1998).…”

Section: Methodsmentioning

confidence: 99%

Engineering Mycobacterium smegmatis for testosterone production

Fernández-Cabezón

Galán

2016

Microbial Biotechnology

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SummaryA new biotechnological process for the production of testosterone (TS) has been developed to turn the model strain Mycobacterium smegmatis suitable for TS production to compete with the current chemical synthesis procedures. We have cloned and overexpressed two genes encoding microbial 17β‐hydroxysteroid: NADP 17‐oxidoreductase, from the bacterium Comamonas testosteroni and from the fungus Cochliobolus lunatus. The host strains were M. smegmatis wild type and a genetic engineered androst‐4‐ene‐3,17‐dione (AD) producing mutant. The performances of the four recombinant bacterial strains have been tested both in growing and resting‐cell conditions using natural sterols and AD as substrates respectively. These strains were able to produce TS from sterols or AD with high yields. This work represents a proof of concept of the possibilities that offers this model bacterium for the production of pharmaceutical steroids using metabolic engineering approaches.

show abstract

Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation

Cited by 117 publications

References 7 publications

Bacterial Flora of the Anopheles fluviatilis S. L., the Vector of Malaria in Southern Iran for Proper Candidate Paraterasgenesis

Bacterial Flora of the Anopheles fluviatilis S. L., the Vector of Malaria in Southern Iran for Proper Candidate Paraterasgenesis

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Engineering Mycobacterium smegmatis for testosterone production

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