Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase

Sepulchro, Ana Gabriela Veiga; Pellegrini, Vanessa de Oliveira Arnoldi; Briganti, Lorenzo; Araújo, Evandro Ares de; Araújo, Simara Semíramis de; Polikarpov, Igor

doi:10.1016/j.carbpol.2020.116714

Cited by 26 publications

(21 citation statements)

References 60 publications

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“…The ratio of xylobiose in the xylose-based sugars released by MxynB-8 was higher than the previously reported 19.56 and 51.6% (Azelee et al, 2016;Sepulchro et al, 2020) and lower than reported 85.99% (Xian et al, 2019). In addition, the enzymatic efficiency based on the poplar sawdust xylan in the raw material was 30.5%, which is slightly higher than or similar to other reported enzymatic hydrolysis results (Azelee et al, 2016;Jacomini et al, 2020;Sepulchro et al, 2020). These results indicate that MxynB-8 has a promising biotechnological potential to transform poplar sawdust xylan into XOS, which can contribute to valorization of underutilized agricultural and forestry wastes.…”

Section: Optimization Of Hydrolysis Conditions Of Poplar Sawdust Xylan By Mxynb-8contrasting

confidence: 77%

“…Among the hydrolysates, xylobiose and xylotriose were the main hydrolysates, accounting for 75.5% (with a concentration of 1.29 g/L in hydrolysate) and 24.3% (with a concentration of 0.42 g/L in hydrolysate) of the total hydrolysates, respectively, and contained a small amount of xylose and xylohexaose. The ratio of xylobiose in the xylose-based sugars released by MxynB-8 was higher than the previously reported 19.56 and 51.6% (Azelee et al, 2016;Sepulchro et al, 2020) and lower than reported 85.99% (Xian et al, 2019). In addition, the enzymatic efficiency based on the poplar sawdust xylan in the raw material was 30.5%, which is slightly higher than or similar to other reported enzymatic hydrolysis results (Azelee et al, 2016;Jacomini et al, 2020;Sepulchro et al, 2020).…”

Section: Optimization Of Hydrolysis Conditions Of Poplar Sawdust Xylan By Mxynb-8contrasting

confidence: 55%

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Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis

Jiang

Tong

et al. 2021

Front. Bioeng. Biotechnol.

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As is well-known, endo-1,4-β-xylanase and β-xylosidase are the rate-limiting enzymes in the degradation of xylan (the major hemicellulosic component), main functions of which are cleavaging xylan to release xylooligosaccharides (XOS) and xylose that these two compounds have important application value in fuel, food, and other industries. This study focuses on enzymatic hydrolysis of poplar sawdust xylan for production of XOS and xylose by a GH11 endo-1,4-β-xylanase MxynB-8 and a GH39 β-xylosidase Xln-DT. MxynB-8 showed excellent ability to hydrolyze hemicellulose of broadleaf plants, such as poplar. Under optimized conditions (50°C, pH 6.0, dosage of 500 U/g, substrate concentration of 2 mg/mL), the final XOS yield was 85.5%, and the content of XOS2−3 reached 93.9% after 18 h. The enzymatic efficiency by MxynB-8 based on the poplar sawdust xylan in the raw material was 30.5%. Xln-DT showed excellent xylose/glucose/arabinose tolerance, which is applied as a candidate to apply in degradation of hemicellulose. In addition, the process and enzymatic mode of poplar sawdust xylan with MxynB-8 and Xln-DT were investigated. The results showed that the enzymatic hydrolysis yield of poplar sawdust xylan was improved by adding Xln-DT, and a xylose-rich hydrolysate could be obtained at high purity, with the xylose yield of 89.9%. The enzymatic hydrolysis yield was higher (32.2%) by using MxynB-8 and Xln-DT together. This study provides a deep understanding of double-enzyme synergetic enzymolysis of wood polysaccharides to valuable products.

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Section: Optimization Of Hydrolysis Conditions Of Poplar Sawdust Xylan By Mxynb-8contrasting

confidence: 77%

Section: Optimization Of Hydrolysis Conditions Of Poplar Sawdust Xylan By Mxynb-8contrasting

confidence: 55%

Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis

Jiang

Tong

et al. 2021

Front. Bioeng. Biotechnol.

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“…Another example is given by xylanase TcXyn10A from Thermobacillus composti, which was characterized by a 60% of relative activity after 8 h of incubation at 65 • C. Moreover, TcXyn10A improved the efficiency of pretreated lignocellulosic biomass hydrolysis when added to the Accellerase ® 1500 cocktail, leading to a 15.35 and 4.38% increased release in xylose and glucose, respectively. Moreover, its resistance toward 10% (v/v) ethanol is noteworthy because it makes this enzyme particularly suitable for one-pot/one-phase thermophilic SSF (see above) [99]. Regarding other types of hemicellulases, the addition of the mannanase MtMan26A from C. thermophilum to the commercial cocktails Celluclast ® 1.5 L and Novozyme ® 188 (no longer commercially available) resulted in enhanced enzymatic hydrolysis of pretreated beechwood sawdust.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production

et al. 2021

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To mitigate the current global energy and the environmental crisis, biofuels such as bioethanol have progressively gained attention from both scientific and industrial perspectives. However, at present, commercialized bioethanol is mainly derived from edible crops, thus raising serious concerns given its competition with feed production. For this reason, lignocellulosic biomasses (LCBs) have been recognized as important alternatives for bioethanol production. Because LCBs supply is sustainable, abundant, widespread, and cheap, LCBs-derived bioethanol currently represents one of the most viable solutions to meet the global demand for liquid fuel. However, the cost-effective conversion of LCBs into ethanol remains a challenge and its implementation has been hampered by several bottlenecks that must still be tackled. Among other factors related to the challenging and variable nature of LCBs, we highlight: (i) energy-demanding pretreatments, (ii) expensive hydrolytic enzyme blends, and (iii) the need for microorganisms that can ferment mixed sugars. In this regard, thermophiles represent valuable tools to overcome some of these limitations. Thus, the aim of this review is to provide an overview of the state-of-the-art technologies involved, such as the use of thermophilic enzymes and microorganisms in industrial-relevant conditions, and to propose possible means to implement thermophiles into second-generation ethanol biorefineries that are already in operation.

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“…xylose by the action of β-xylosidase, and other enzymes in xylanase such as acetylxylan esterase and other side chain hydrolases usually have synergistic effects on the main chain hydrolases. Through the cooperation of multiple enzymes finally hydrolyze xylan into xylose [9].…”

Section: Introductionmentioning

confidence: 99%

Characteristic of a GH10 Xylanase From The Anaerobic Rumen Fungus Anaeromyces Robustus and Application in Bread Making

Zhen-yang

Wen

et al. 2021

Preprint

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The rumen of ruminants contains a variety of fungi capable of producing xylanases to break down plant cell walls. In this study, a new GH10 xylanase gene ArXyn10c20 from anaerobic rumen microorganism Anaeromyces robustus was successfully expressed in Pichia Pastoris GS115, with a protein molecular weight of approximately 42 kDa and showed the similarity by 64.08% with the β-Xylanase form Neocallimastix Californiae. The optimal pH and temperature for ArXyn10c20 was 5.5 at 40℃. ArXyn10c20 was stable in the pH range 5.0 – 9.0 for 1h which the residual enzyme activity was all above 75%. The activity of recombinant xylanase was significantly enhanced by 1 mM Cu 2+ . The products of ArXyn10c20 hydrolysis of beechwood xylan were xylobiose, xylotriose and xylotetraose by TLC analysis. In food applications, ArXyn10c20 can significantly improve the quality of dough and bread. With the addition of 7.5 mg ArXyn10c20, the hardness, gumminess and chewiness of the bread decreased by 42.24%, 45.33% and 55.36% respectively and the reducing sugar increased by 18.67%. The new discovered xylanase ArXyn10c20 has great potential in food industry.

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Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase

Cited by 26 publications

References 60 publications

Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis

Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis

Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production

Characteristic of a GH10 Xylanase From The Anaerobic Rumen Fungus Anaeromyces Robustus and Application in Bread Making

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