1983
DOI: 10.1271/bbb1961.47.1691
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Transformation of yeast cells treated with 2-mercaptoethanol.

Abstract: The transformation of yeast cells has been carried out by using protoplasts prepared by treatment with lytic enzymes. Recently,1} we have found that plasmid DNAswere taken up by yeast cells treated with alkali cations such as Li+, Na+ K+, Rb+ and Cs+. During a study on the DNA uptake mechanisms of intact yeast cells treated with such monovalent cations, we found that the yeast cells treated with 2-mercaptoethanol (2-ME) could also

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Cited by 479 publications
(507 citation statements)
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“…Yeast two hybrid system YPB2 cells were transformed to tryptophan prototrophy with pXRB26 (Bustelo et al, 1995) by the alkaline method (Ito et al, 1983). A single Trp + colony from this transfection was grown in SC-Trp medium and transformed with a human B-cell cDNA library, as previously described (Bustelo et al, 1995).…”
Section: Antibodiesmentioning
confidence: 99%
“…Yeast two hybrid system YPB2 cells were transformed to tryptophan prototrophy with pXRB26 (Bustelo et al, 1995) by the alkaline method (Ito et al, 1983). A single Trp + colony from this transfection was grown in SC-Trp medium and transformed with a human B-cell cDNA library, as previously described (Bustelo et al, 1995).…”
Section: Antibodiesmentioning
confidence: 99%
“…Sandmeyer). Plasmids were transformed into yeast by the method of Ito [29]. Synthetic medium used for plasmid selection was made according to standard protocols.…”
Section: Strains and Culture Conditionsmentioning
confidence: 99%
“…Transformation of S. pombe was done as in [10] and transformation of S. cerevisiae as in [13]. Genomic DNA from S, pombe was obtained as in [14].…”
Section: Bacterial Strains Dna and Rna Manipulationsmentioning
confidence: 99%