Transformation with a gene for myo-inositol O-methyltransferase enhances the cold tolerance of Arabidopsis thaliana

Zhu, Bangshang; Peng, Ri‐He; Xiong, Ai‐Sheng; Xu, Jingya; Fu, Xin; Wang, Zhao; Jin, Xin; Meng, X. R.; Gao, Jian‐Jie; Cai, Run; Yao, Qizheng

doi:10.1007/s10535-012-0029-y

Cited by 13 publications

(4 citation statements)

References 22 publications

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“…The expression profile of Comp3048, which codes for a methyltransferase, maintained high levels during drought stress, especially on D2. It was found that myo-inositol-O-methyltransferase (Imt1) responded to low temperature stress in transgenic Arabidopsis [37] . Trithorax-like H3K4 methyltransferase from barley is drought inducible [38] .…”

Section: Resultsmentioning

confidence: 99%

Analysis of the Citrullus colocynthis Transcriptome during Water Deficit Stress

Wang

Goertzen

et al. 2014

PLoS ONE

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Citrullus colocynthis is a very drought tolerant species, closely related to watermelon (C. lanatus var. lanatus), an economically important cucurbit crop. Drought is a threat to plant growth and development, and the discovery of drought inducible genes with various functions is of great importance. We used high throughput mRNA Illumina sequencing technology and bioinformatic strategies to analyze the C. colocynthis leaf transcriptome under drought treatment. Leaf samples at four different time points (0, 24, 36, or 48 hours of withholding water) were used for RNA extraction and Illumina sequencing. qRT-PCR of several drought responsive genes was performed to confirm the accuracy of RNA sequencing. Leaf transcriptome analysis provided the first glimpse of the drought responsive transcriptome of this unique cucurbit species. A total of 5038 full-length cDNAs were detected, with 2545 genes showing significant changes during drought stress. Principle component analysis indicated that drought was the major contributing factor regulating transcriptome changes. Up regulation of many transcription factors, stress signaling factors, detoxification genes, and genes involved in phytohormone signaling and citrulline metabolism occurred under the water deficit conditions. The C. colocynthis transcriptome data highlight the activation of a large set of drought related genes in this species, thus providing a valuable resource for future functional analysis of candidate genes in defense of drought stress.

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Section: Resultsmentioning

confidence: 99%

Analysis of the Citrullus colocynthis Transcriptome during Water Deficit Stress

Wang

Goertzen

et al. 2014

PLoS ONE

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“…Alternatively, some of these key enzymes have been disrupted in their action for comparison purposes by means of the construction of DNA-tagged knockout, or RNA interference mutants. Some of these key enzymes include aldehyde dehydrogenases [40], [67], [73], [78], [101], [110], Alkenal Oxidoreductase (AOR) [83], aldo-keto-reductase [87], [88], [91] and myo-inositol-O-methyltransferase (Imt1) [106], among others. Although most of these studies have been performed in Arabidopsis thaliana , these assays are being extended to plants of further agronomical interest, as initially performed in groundnut [60] by the creation of transgenic plants co-expressing aldo-keto reductase (AKR1) and protein L-isoaspartyl methyltransferase (PIMT2), or in rice and tobacco plants overexpressing AKR1 [61].…”

Section: Future Perspectivesmentioning

confidence: 99%

A concise appraisal of lipid oxidation and lipoxidation in higher plants

Alché

2019

Redox Biology

175

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Polyunsaturated fatty acids present in plant membranes react with reactive oxygen species through so-called lipid oxidation events. They generate great diversity of highly-reactive lipid-derived chemical species, which may be further degraded enzymatically or non-enzymatically originating new components like Reactive Carbonyl Species (RCS). Such RCS are able to selectively react with proteins frequently producing loss of function through lipoxidation reactions. Although a basal concentration of lipoxidation products exists in plants (likely involved in signaling), their concentration and variability growth exponentially when plants are subjected to biotic/abiotic stresses. Such conditions typically increase the presence of ROS and the expression of antioxidant enzymes, together with RCS and also metabolites resulting from their reaction with proteins (advanced lipoxidation endproducts, ALE), in those plants susceptible to stress. On the contrary, plants designed as resistant may or may not display enhanced levels of ROS and antioxidant enzymes, whereas levels of lipid oxidation markers as malondialdehyde (MDA) are typically reduced. Great efforts have been made in order to develop methods to identify and quantify RCS, ALE, and other adducts with high sensitivity. Many of these methods are applied to the analysis of plant physiology and stress resistance, although their use has been extended to the control of the processing and conservation parameters of foodstuffs derived from plants. These foods may accumulate either lipid oxidation/lipoxidation products, or antioxidants like polyphenols, which are sometimes critical for their organoleptic properties, nutritional value, and health-promoting or detrimental characteristics. Future directions of research on different topics involving these chemical changes are also discussed.

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“…Recently, Zhu et al [41] used a similar strategy as the one developed by Sheveleva et al [39] to express in A. thaliana, a gene coding IMT1, from Mesembryanthemum crystallinum. The transformed plants showed higher growth compared to the wild control line and increased tolerance to cold stress (4°C).…”

Section: Methylated Ins-derivatives In Plant Cell and The Biotechnolomentioning

confidence: 99%

The Transcriptional Modulation of Inositols and Raffinose Family Oligosaccharides Pathways in Plants — An (A)Biotic Stress Perspective

Neto¹,

Souza²,

Benko‐Iseppon³

et al. 2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

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Among the multifunctional molecules that participate in processes of plant tolerance/ resistance to stresses, inositol (Ins) and its derivatives (phosphorylated, methylated, oxygenated, and Raffinose Family Oligosaccharides) have attracted the attention of researchers. These compounds represent versatile and dynamic signaling molecules and osmolytes in all eukaryotes. Due to the impacts related to Ins and its derivatives in a plant cell, assays have been conducted to understand how these biomolecules affect plant physiology. Thus, overexpression or knockout of Ins-related genes has been shown as interesting strategies for generating more efficient plants capable of growing under stress conditions. In this chapter, studies using molecular tools are presented, and the impacts of their results are discussed based on the plant stress tolerance/resistance. Furthermore, an informative panel is provided with transcriptional modulation of genes related to Ins and its derivatives expressed in plants under stress. There is a gap involving about two dozen enzymes associated with the synthesis of Ins-related compounds that have not been adequately studied, and they represent an area of high biotechnological potential.

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Transformation with a gene for myo-inositol O-methyltransferase enhances the cold tolerance of Arabidopsis thaliana

Cited by 13 publications

References 22 publications

Analysis of the Citrullus colocynthis Transcriptome during Water Deficit Stress

Analysis of the Citrullus colocynthis Transcriptome during Water Deficit Stress

A concise appraisal of lipid oxidation and lipoxidation in higher plants

The Transcriptional Modulation of Inositols and Raffinose Family Oligosaccharides Pathways in Plants — An (A)Biotic Stress Perspective

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