Transformed T lymphocytes infected by a novel isolate of human T cell leukemia virus type II

Chorba, TL; Brynes, R; Kalyanaraman, V. S.; Telfer, M; Ramsey, R; Mawle, A; El, Palmer; Chen, AT; Feorino, P; Evatt, BL

doi:10.1182/blood.v66.6.1336.1336

Cited by 7 publications

(2 citation statements)

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“…It has the same type of transacting mechanism as HTLV-I and produces a tat gene product. HTLV-I1 has also been isolated from a hemophiliac patient with pancytopenia (Chorba et al, 1985) and from an AIDS patient, but the significance of this retrovirus in human disease has not been determined.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of human retroviruses

Palmer¹,

Goldsmith²

1988

J. Elec. Microsc. Tech.

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We compared the ultrastructure of the human retroviruses by thin-section electron microscopy of infected lymphocytes. Virus particles form at the plasma membrane without involvement of a cytoplasmic precursor. Budding forms of human T-cell leukemia virus types I and II (HTLV-I and -II) consist of a crescent-shaped nucleoid separated from the envelope by an intermediate layer. Mature forms of these viruses are about 100 nm in diameter. The nucleoid is electron lucent and almost completely fills the virion. There is about a 10-nm space between the envelope and nucleoid. The envelope has fuzzy surface projections. HTLV-I and -II resemble other type C retroviruses in morphology. Budding forms of human immunodeficiency virus (HIV, LAV, HTLV-III) also have a crescent-shaped nucleoid but not an intermediate layer between the core and envelope. The envelope has rod-shaped surface projections. Mature forms of HIV have an electron-dense nucleoid that is eccentric and bar- or cone-shaped. Particles have the same ultrastructure as retroviruses of the Lentivirus genus. HIV is readily distinguishable from HTLV-I and -II by thin-section electron microscopy. HIV is usually found in extracellular spaces by transmission electron microscopy of thin sections, and scanning electron microscopy of HIV-infected T4 lymphocytes also shows many particles on the surface of these cells. Lymphadenopathy-associated virus type II (LAV-II) has the same internal ultrastructure as HIV, but its surface projections are more prominent, being about three times the length of those of HIV. Human T lymphotropic virus type IV (HTLV-IV) has the same morphology as LAV-II.

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Section: Introductionmentioning

confidence: 99%

Ultrastructure of human retroviruses

Palmer¹,

Goldsmith²

1988

J. Elec. Microsc. Tech.

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“…Serologically, it is difficult to distinguish between HTLV-I and HTLV-II because their relatively high amino acid sequence homology results in substantial crossreactivity (15). However, the HTLV-II particles with the less dense cores observed by Kalyanaraman et a/.…”

Section: Discussionmentioning

confidence: 99%

Human Helper T Cell Lines Established by Coculture of Normal Human Cord Leukocytes with an HTLV‐Il‐infected Rabbit Leukocyte Cell Line (Ra‐IIA)

Hayashi

Ohara

Fujiwara

et al. 1992

Acta Pathologica Japonica

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Three helper T cell lines, designated CR ‐IIA (CR‐IIA‐1, CR‐IIA‐ 2, and CR‐IIA‐3), were established by coculturing nor mal human cord leukocytes with a lethally irradiated HTLV II (human T lymphotropic virus type II)‐ infected rabbit leukocyte cell line (Ra‐IIA). CR IIA had a normal human karyotype and expressed the surface markers CD3(+), CD4(+), CD8(‐), CD19(‐), CD25(+) and HLA‐ DR(+), confirming their helper T cell nature. CR‐ IIA cells were all free of Epstein‐ Barr virus nuclear antigen and were im‐muno reactive with serum samples from HTLV‐ I‐ or HTLV‐Il‐ infected patients and with anti HTLV‐ I, p19 or p24 anti body. The provirus genome of HTLV‐II was detected in these cell lines by the polymerase chain reaction combined with a digoxigenin‐ enzyme‐ linked immunosorbent assay. Electron microscopy of CR‐IIA‐1 cells revealed a few im mature type C virus particles. These results suggest that HTLV‐ II was transmitted from the infected rabbit leukocyte cell line to human cord helper T lymphocytes with the development of immortalized HTLV ‐ II‐ producing T cell lines. Acta Pathol Jpn 42: 347–352, 1992.

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HIV Culture

Rayfield¹

1994

AIDS Testing

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Transformed T lymphocytes infected by a novel isolate of human T cell leukemia virus type II

Cited by 7 publications

References 0 publications

Ultrastructure of human retroviruses

Ultrastructure of human retroviruses

Human Helper T Cell Lines Established by Coculture of Normal Human Cord Leukocytes with an HTLV‐Il‐infected Rabbit Leukocyte Cell Line (Ra‐IIA)

HIV Culture

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