Transforming growth factor-beta: Vasculogenesis, angiogenesis, and vessel wall integrity

Pepper, Michael Sean

doi:10.1016/s1359-6101(96)00048-2

Cited by 638 publications

(464 citation statements)

References 184 publications

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“…For example, neutralizing TGF-␤ antibody infusion in neonatal rats delays in vivo glomerular capillary formation (Liu et al, 1999), while embryonic tissues in TGF-␤ knockout mice exhibit normal vascular structures in embryonic, but not extraembryonic tissues (Dickson et al, 1995). Data from in vitro studies, using various endothelial cell lines show an inhibitory effect of TGF-␤ on proliferation (reviewed by Pepper, 1997). However, our explant model, as noted earlier, is not limited to endothelial cells.…”

Section: Discussionmentioning

confidence: 91%

“…The precise role of TGF-␤ in vasculogenesis is not yet documented, and, as noted in a review by Pepper (1997), may be dictated by a number of variables. Its Fig.…”

Section: Discussionmentioning

confidence: 99%

“…This hypothesis was based on the rationale that angiopoietins, along with VEGF are major regulators in developmental systems (Hayes et al, 1999;Koblizek et al, 1998;Suri et al, 1996). Finally, we tested the hypothesis that TGF-␤ 1 inhibits coronary vasculogenesis, since in vitro experiments using cell lines have shown it to inhibit endothelial cell growth, migration and capillary formation (Li et al, 2000;Pepper, 1997). However, the role of TGF-␤ 1 in vasculogenesis/angiogenesis is controversial, since this growth factor has been found to induce VEGF expression (Li et al, 1997;Seko et al, 1999;Zheng et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Multiple growth factors regulate coronary embryonic vasculogenesis

Tomanek

Zheng

Peters

et al. 2001

Developmental Dynamics

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Mechanisms regulating coronary vascularization are not well understood. To test hypotheses regarding the influence of key growth factors and their interactions, we studied vascular tube formation (vasculogenesis) in collagen gels onto which quail embryonic ventricles were placed and incubated in the presence of growth factors or inhibitors. Vasculogenesis in this model is dependent on tyrosine kinase receptors, since tube formation was totally blocked by genestein. Tube formation was attenuated when anti-bFGF or anti-VEGF neutralizing antibodies were added to the medium and nearly completely inhibited when the both were added. The attenuation associated with anti-VEGF was due primarily to a decrease in assembly of endothelial cells, while that associated with bFGF was primarily due to a reduction in endothelial cells. Soluble tie-2, the receptor for angiopoietins, also had an inhibitory effect and, when added with either anti-bFGF or anti-VEGF, markedly attenuated tube formation. At optimal doses, tube formation was enhanced 6.5-fold by bFGF and 2.5-fold by VEGF over the controls. Each of these growth factors was dependent upon the other for optimal induction of tube formation, since neutralizing antibodies to one markedly reduced the potency of the other. VEGF potency was also markedly reduced when soluble tie-2 was added to the medium. Tube formation was virtually totally blocked by exogenous TGF-␤ at doses > 1 ng/ml, while neutralizing TGF-␤ antibodies enhanced tube formation 2-fold in the 30 ng-30 g range. These data provide the first documentation of multiple growth factor regulation of coronary tube formation.

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Section: Discussionmentioning

confidence: 91%

“…The precise role of TGF-␤ in vasculogenesis is not yet documented, and, as noted in a review by Pepper (1997), may be dictated by a number of variables. Its Fig.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple growth factors regulate coronary embryonic vasculogenesis

Tomanek

Zheng

Peters

et al. 2001

Developmental Dynamics

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“…A number of studies support the concept that TGF-␤1 regulates endothelial cell growth and angiogenesis (Pepper, 1997). The targeted disruption of TGF-␤1 gene led to early embryonic lethality in about 50% of homozygous as a result of the defects in the yolk sac vasculature and the haematopoietic system (Martin et al, 1995).…”

Section: Discussionmentioning

confidence: 95%

“…Similarly, the targeted inactivation of TGF-␤ receptor T␤RII also resulted in defects in yolk sac vasculogenesis and haematopoiesis in homozygous embryos (Oshima et al, 1996). TGF-␤1 strongly induced angiogenesis when administered subcutaneously to newborn or adult mice or rat (Pepper, 1997). However, the angiogenesis induced by TGF-␤1 in these in vivo assays was usually associated with inflammation and tissue repair.…”

Section: Discussionmentioning

confidence: 99%

TGF‐β1 perturbs vascular development and inhibits epithelial differentiation in fetal lung in vivo

Zeng

Gray

Stahlman

et al. 2001

Developmental Dynamics

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Members of the transforming growth factor ␤ (TGF-␤) family of polypeptides have been implicated in morphogenesis and differentiation in numerous tissues, including the lung. In order to further define effects of TGF-␤ signaling in lung morphogenesis, a constitutively active form of TGF-␤1 was expressed in respiratory epithelial cells of the fetal mouse lung in vivo. Expression of TGF-␤1 arrested lung morphogenesis in the pseudoglandular stage of development, inhibiting synthesis of differentiation-dependent proteins, SP-B, SP-C, and CCSP, and maintaining embryonic patterns of staining for thyroid transcription factor-1 (TTF-1) and hepatocyte nuclear factor-3␤ (HNF-3␤). The pulmonary mesenchyme was thickened and vascular density was increased by TGF-␤1. TGF-␤1 decreased expression of vascular endothelial growth factor-A (VEGF-A) mRNA and protein, and the abundance of Flk-1 mRNA in the lung mesenchyme. Distribution of platelet-endothelial cell adhesion molecule (PECAM)-1, a marker of pulmonary blood vessels, was altered, and ultrastructural studies demonstrated that TGF-␤1 inhibited vascular development in the fetal lung. TGF-␤1 perturbed both epithelial cell differentiation and formation of the pulmonary vasculature, supporting the concept that precise control of signaling via the TGF-␤ receptor pathway is critical for normal lung morphogenesis.

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Ontogeny of the opioid growth factor, [Met5]-enkephalin, preproenkephalin gene expression, and the ζ opioid receptor in the developing and adult aorta of rat

McLaughlin

Zagon

1998

Dev. Dyn.

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Transforming growth factor-beta: Vasculogenesis, angiogenesis, and vessel wall integrity

Cited by 638 publications

References 184 publications

Multiple growth factors regulate coronary embryonic vasculogenesis

Multiple growth factors regulate coronary embryonic vasculogenesis

TGF‐β1 perturbs vascular development and inhibits epithelial differentiation in fetal lung in vivo

Ontogeny of the opioid growth factor, [Met5]-enkephalin, preproenkephalin gene expression, and the ζ opioid receptor in the developing and adult aorta of rat

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