Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells

Serita, S.; Tomokiyo, Atsushi; Hasegawa, Daigaku; Sugii, Hideki; Yoshida, Shinichiro; Mizumachi, Hiroyuki; Mitarai, Hiromi; Monnouchi, Satoshi; Wada, Naohisa; Maeda, Hidefumi

doi:10.1016/j.archoralbio.2017.02.018

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…We recently showed that DM promoted odontoblastic differentiation of HDPCs (Mizumachi et al, ; Serita et al, ). To examine the effects of TNF‐α on the odontoblastic differentiation of yHDPCs and sHDPCs, the cells were cultured in CM or DM with or without TNF‐α for 7 days.…”

Section: Resultsmentioning

confidence: 99%

“…We recently showed that DM promoted odontoblastic differentiation of HDPCs (Mizumachi et al, 2017;Serita et al, 2017). To…”

Section: Effects Of Tnf-α On the Odontoblastic Differentiation Of Hdpcsmentioning

confidence: 95%

“…HDPCs were cultured using three different medium conditions: 10% FBS-α-MEM as control culture medium (CM), CM containing 2 mM CaCl 2 as differentiation medium (DM), or DM containing TNF-α (DM + TNF-α). Our recent studies showed that DM promoted odontoblastic differentiation of HDPCs (Mizumachi et al, 2017;Serita et al, 2017). After culturing, the cells were subjected to Alizarin Red S staining and polymerase chain reaction (PCR) analysis.…”

Section: Odontoblastic Differentiation Of Hdpcsmentioning

confidence: 99%

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Senescence and odontoblastic differentiation of dental pulp cells

Nozu

Tomokiyo

Hasegawa

et al. 2018

Journal Cellular Physiology

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Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration of tumor necrosis factor-α (TNF-α) is higher in the blood of aged people compared with that of young people. So far, the precise effects of TNF-α on the odontoblastic differentiation of pulp cells have been controversial. Therefore, we aimed to clarify how this cytokine affected pulp cells during aging. Human dental pulp cells (HDPCs) were cultured until reaching the plateau of their growth, and the cells were isolated at actively (young HDPCs; yHDPCs) or inactively (senescent HDPCs; sHDPCs) proliferating stages. sHDPCs expressed senescence-related molecules while yHDPCs did not. When these HDPCs were cultured in an odontoblast-inductive medium, both young and senescent cells showed mineralization, but mineralization in sHDPCs was lower compared with yHDPCs. However, the administration of TNF-α to this culture medium altered these responses: yHDPCs showed downregulated mineralization, while sHDPCs exhibited significantly increased mineralization. Furthermore, the expression of tumor necrosis factor receptor 1 (TNFR1), a receptor of TNF-α, was significantly upregulated in sHDPCs compared with yHDPCs. Downregulation of TNFR1 expression led to decreased mineralization of TNF-α-treated sHDPCs, whereas restored the reduction in TNF-α-treated yHDPCs. These results suggested that sHDPCs preserved the odontoblastic differentiation capacity and TNF-α promoted odontoblastic differentiation of HDPCs with the progress of their population doublings through increased expression of TNFR1. Thus, TNF-α might exert a different effect on the odontoblastic differentiation of HDPCs depending on their proliferating activity. In addition, the calcification of pulp chamber with age may be related with increased reactivity of pulp cells to TNF-α.

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Section: Resultsmentioning

confidence: 99%

“…We recently showed that DM promoted odontoblastic differentiation of HDPCs (Mizumachi et al, 2017;Serita et al, 2017). To…”

Section: Effects Of Tnf-α On the Odontoblastic Differentiation Of Hdpcsmentioning

confidence: 95%

Section: Odontoblastic Differentiation Of Hdpcsmentioning

confidence: 99%

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Senescence and odontoblastic differentiation of dental pulp cells

Nozu

Tomokiyo

Hasegawa

et al. 2018

Journal Cellular Physiology

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“…MTA has been reported to release calcium ions [39,40] and induce reparative dentin after the pulp exposure when it is applied as a direct pulp capping material [41][42][43][44]. We previously reported that CaCl 2 induces mineralization and odontoblastic differentiation of dental pulp cells [45]. As for the M2 macrophages, recent studies reported that M2 macrophages induce mineralization of the mesenchymal stem cells [46,47].…”

Section: Discussionmentioning

confidence: 99%

Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation

Kadowaki

Yoshida

Itoyama

et al. 2022

Life

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In cases in which dental pulp tissue is accidentally exposed, direct pulp capping is often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and tissue repair, the emergence pattern and the role of macrophages in dental pulp tissue have not been clarified. Here, we investigated the emergence of M1/M2 macrophages in dental pulp tissue after a direct pulp capping and the effects of M2 macrophages on odontoblastic differentiation of the dental pulp stem cell (DPSC) clones. The emergence of macrophages in dental pulp tissue was investigated using a rat direct pulp capping model. Alizarin Red S staining and quantitative RT-PCR was performed to examine the effect of M2 macrophages on the mineralization and odontoblastic differentiation of DPSC clones. Immunohistochemical staining revealed that M1 macrophages were detected in dental pulp tissue after treatment and increased in number at three days after treatment. However, M2 macrophages gradually increased in number in dental pulp tissue after treatment, with the highest level recorded at seven days post-operation. Additionally, conditioned medium from M2 macrophages induced odontoblast-like differentiation of DPSC clones. These results suggest that macrophages play a role in the inflammatory response and reparative dentin formation after dental pulp exposure.

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“…The same research group also showed that during experimental tooth movement of human premolars, βigh3 expression was significantly increased in the PDL [ 105 ]. Recently, βigh3 was also found to be expressed in rat pulp, where it could be associated with reparative dentinogenesis pointing to the potential therapeutic role of βigh3 in the pulpal repair process [ 106 ].…”

Section: Gla-containing Ecm Proteinsmentioning

confidence: 99%

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

Nikoloudaki

2021

IJMS

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Matricellular proteins (MCPs) are defined as extracellular matrix (ECM) associated proteins that are important regulators and integrators of microenvironmental signals, contributing to the dynamic nature of ECM signalling. There is a growing understanding of the role of matricellular proteins in cellular processes governing tissue development as well as in disease pathogenesis. In this review, the expression and functions of different MP family members (periostin, CCNs, TSPs, SIBLINGs and others) are presented, specifically in relation to craniofacial development and the maintenance of orofacial tissues, including bone, gingiva, oral mucosa, palate and the dental pulp. As will be discussed, each MP family member has been shown to have non-redundant roles in development, tissue homeostasis, wound healing, pathology and tumorigenesis of orofacial and dental tissues.

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Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells

Abstract: The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process.

Cited by 7 publications

References 35 publications

Senescence and odontoblastic differentiation of dental pulp cells

Senescence and odontoblastic differentiation of dental pulp cells

Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

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