Transforming Growth Factor β1 Stimulation of Progesterone Production in Cultured Granulosa Cells from Gonadotropin-Primed Adult Rats

Ohmura, Hiroshi; Chang, William Y.; Uzumcu, Mehmet; Coşkun, Serdar; Akira, Shigeo; Araki, Tsutomu; Lin, Yi‐Chia

doi:10.1007/978-1-4613-9260-6_16

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“…Although TGF-P alone had no effect on spontaneous rat oocyte maturation, it suppressed LH-induced rat oocyte maturation in vitro (Tsafriri et al, 1989). Diverse effects of TGF-P between rats and pigs have been reported for progesterone production Ohmura et al, 1993) and gonadotropin receptor regulation on granulosa cells (Gitay-Goren et al, 1993). TGF-P was detected in follicular fluid of small-size porcine follicles ( May, 1990) and found to inhibit FSH-stimulated progesterone production and basal thymidine incorporation by porcine granulosa cells (Mondschein et al, 1988).…”

Section: Discussionmentioning

confidence: 92%

Effects of transforming growth factors and activin‐A on in vitro porcine oocyte maturation

Coşkun

Lin

1994

Molecular Reproduction Devel

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Growth factors are known to regulate ovarian function. In the present study, effects of these growth factors, TGF-alpha, TGF-beta, and activin-A were tested on spontaneous porcine oocyte maturation. Cumulus-oocyte complexes (COC) were cultured in the presence of TGF-alpha, TGF-beta, and activin-A for 48 hr. Stages of meiotic maturation were assessed by staining with acetic orcein. Among these factors, only TGF-alpha significantly enhanced the maturation rate, whereas TGF-beta suppressed the spontaneous maturation rate. The site of action of TGF-alpha on COC and the interaction between TGF-alpha and EGF receptor was also examined. Denuded oocytes, alone or in coculture with cumulus cells, were cultured in the presence of TGF-alpha for 48 hr. TGF-alpha did not have any significant effect on denuded oocyte maturation. Heptanol was employed to investigate the role of gap junctions on TGF-alpha-induced oocyte maturation in COC. Although heptanol did not have any significant effect in the control medium, heptanol reversed the stimulatory effect of TGF-alpha on porcine oocyte maturation. TGF-alpha was able to displace 125I-EGF binding on COC. In conclusion, TGF-alpha enhances the spontaneous maturation of porcine oocytes by generating positive signal(s) in cumulus cells that are transferred to the oocyte via gap junctions. TGF-alpha shares the same receptor with EGF on porcine COC. TGF-beta, in contrast, inhibits porcine oocyte maturation.

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