Transforming sugars into fat - lipid biosynthesis using different sugars inYarrowia lipolytica

Abstract

In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass-derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae and INU1 from Kluyveromyces marxianus. The second strain, Y. lipolytica Y4779, was derived from the Polish A-101 strain. It had inhibited β-oxidation (∆mfe2) and overexpressed GPD1, DGA1, HXK1, YHT3, SUC2 and INU1. In the first experiment, strain YLZ150 was batch-cultured in media containing different hexoses; the highest values for lipid concentration and yield of lipids from the substrate were obtained using fructose (20.3 g dm and 0.14 g g , respectively). In the second experiment, we grew the two strains in fed-batch cultures to examine lipid biosynthesis from inulin (a fructose polymer). For Y4779, the lipid concentration was 10.3 g dm and the yield of lipids from substrate was 0.07 g g ; in contrast, for YLZ150, these values were 24 g dm and 0.16 g g , respectively. The YLZ150 strain is thus able to efficiently exploit glucose, fructose, galactose, sucrose and inulin for lipid biosynthesis. Copyright © 2017 John Wiley & Sons, Ltd.

Production of high titer of citric acid from inulin

“…Instantly growing IN market results in increasing amounts of inulin-rich wastes after its production that must be managed. One of the possibilities to utilize these raw materials is microbiological production of bioethanol, single-cell protein, lipids, CA, butanediol or lactic acid [28–32].…”

Yarrowia lipolytica strains and their biotechnological applications: how natural biodiversity and metabolic engineering could contribute to cell factories improvement

“…Besides the use of crude glycerol (a by-product of industrial production of bioethanol or soap) and of waste cooking oils (recycled from fast-food and other catering establishments), which both are natural substrates for Y. lipolytica, genetic engineering can also allow the use of lignocellulosic hydrolysates from agriculture, forestry and paper industry, of starch-or inulin-rich agricultural wastes, of molasses from sugar industry and of acid whey from cheese and yogurt industries [50,51]. Interestingly, the Wroclaw University of Environmental and Life Sciences (UPWr, Poland) collaborated with INRA for the design of GM Y. lipolytica strains combining an obese phenotype with a wide substrate range [54]. Notably, these teams engineered GM derivatives of either W29 or A-101 wild-type isolates to produce at first obese strains with increased lipid biosynthesis and storage, and then to add several biomass-derived sugars (galactose, fructose, sucrose and inulin) to the range of substrates that these strains can effectively use (cf.…”

“…The W29-derived YLZ150 strain, which can perform very efficient lipid biosynthesis from a wide range of biomass-derived sugars, is a valuable chassis for SCO or biofuel production from non-lipid renewable resources. In contrast, the A-101-derived Y4779 strain would be more adapted to developing sustainable processes for citric acid or polyhydroxy alcohol production [54].…”

Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

“…The same group also individually screened members of the sugar transporter family for their hexose transport ability using an appropriate heterologous host and identified two active fructose transporters in Y. lipolytica ( Lazar et al, 2017 ). Based on these findings, one promising strain of Y. lipolytica was developed to consume different hexoses via a combination of the above-mentioned strategies ( Hapeta et al, 2017 ). The highest values for lipid concentration and yield of lipids from the fructose reached 20.3 g/L and 0.14 g/g, respectively.…”