Transfusion safety on the African continent: an international quality control of virus testing in blood banks

Laperche, Syria; Boukatou, Geneviève; Kouegnigan, Léonard; Nébié, Yacouba; Boulahi, Mohamed Ould; Tagny, Claude Tayou; Yahaya, Rakia; Tapko, J. B.; Murphy, Edward L.; Lefrère, J.‐J.

doi:10.1111/j.1537-2995.2009.02239.x

Cited by 44 publications

(54 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We used rapids tests which, in a recent international quality control of blood-borne virus testing carried out in six African blood banks, have been shown to have poor sensitivity compared to enzyme immunoassays (EIAs) for the detection of HCV antibodies and HBsAg. 30 In terms of specificity, we did not perform confirmatory testing for anti-HCV-and HBsAg-positive samples, and therefore we may have overestimated the prevalence of HBV and HCV infections through the inclusion of some false-positives.…”

Section: Discussionmentioning

confidence: 97%

Sero-epidemiology of human immunodeficiency virus, hepatitis B and C viruses, and syphilis infections among first-time blood donors in Edéa, Cameroon

Noubiap¹,

Joko

Nansseu

et al. 2013

International Journal of Infectious Diseases

112

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

Sero-epidemiology of human immunodeficiency virus, hepatitis B and C viruses, and syphilis infections among first-time blood donors in Edéa, Cameroon

Noubiap¹,

Joko

Nansseu

et al. 2013

International Journal of Infectious Diseases

112

View full text Add to dashboard Cite

show abstract

“…Blood testing was performed at the University Teaching Hospital blood bank in Yaoundé which has participated in several studies of viral testing (Laperche et al, 2009, 2013). Before starting the study, a panel of 25 known HCV Ab positive sera and 25 negative samples was tested with the HCV Ag/Ab assay to evaluate the performance of technicians and equipment.…”

Section: Methodsmentioning

confidence: 99%

Screening for hepatitis C virus infection in a high prevalence country by an antigen/antibody combination assay versus a rapid test

Tagny

Mbanya

Murphy

et al. 2014

Journal of Virological Methods

Self Cite

View full text Add to dashboard Cite

In low-income-countries, screening for hepatitis C virus (HCV) infection is often based on rapid tests (RT). Their lower sensitivity compared to enzyme immunoassay (EIA) suggests that newer HCV Antigen/Antibody (Ag/Ab) combination assays might have a role in such countries. To test this idea, 1998 blood donors were tested at the University Teaching Hospital blood bank in Yaoundé, Cameroon simultaneously with a RT (HCV rapid test, Human Diagnostics, Berlin, Germany) according to standard practice (S1) and with an Ag/Ab assay (Monolisa HCV Ag/Ab Ultra, Biorad, France) (S2). All discordant, borderline and reactive samples were submitted to confirmatory testing by immunoblot and/or HCV-RNA. Of the 86 (4.3%) samples positive with one or both strategies, 29 were confirmed negative, 37 positive and 20 were false positive or resolved infection. There was a significant difference in test sensitivity (p = 0.01) between S1 (70.3%) and S2 (91.9%) but not in test specificity (99.4% and 98.6%, respectively). The benefit of the Ag/Ab assay in the detection of recent HCV seronegative infections could not be evaluated since no Antigen-only donations were identified. However, better Ag/Ab test sensitivity compared to RT supports the implementation of these newer immunoassays for HCV screening in the African blood bank setting.

show abstract

“…Pendant cette période, sept projets ont été réalisés à l'échelon national et publiés [3,[6][7][8][9][10][11] et cinq travaux multicentriques ont été conduits et publiés [12][13][14][15][16]. Seuls ces derniers sont rapportés ci-dessous.…”

Section: Travaux Réalisés Par Le Groupeunclassified

“…Le panel de la première étude [13] a été soumis aux centres de six pays. Le niveau de qualité global du dépistage du VIH était de 98 %, avec des variations de 92 % à 100 % selon les laboratoires.…”

Section: Stratégies Et Contrôle De Qualité De La Qualification Biologunclassified

The Francophone Africa Blood Transfusion Research Network: a five‐year report (2007–2012)

Tagny

Murphy

Lefrère

2013

Transfusion Medicine

View full text Add to dashboard Cite

Peer reviewed eScholarship.org Powered by the California Digital Library University of CaliforniaThis article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. RésuméLes travaux de recherches sur la sécurité transfusionnelle en Afrique sub-saharienne sont peu nombreux, souvent limités à des initiatives locales avec des conclusions difficilement représentatives de cette région. Le Groupe de recherches transfusionnelles en Afrique sub-saharienne francophone a été créé en mai 2007 avec pour objectif de développer des stratégies globales d'amélioration de la sécurité transfusionnelle mais adaptables à la situation de chaque pays. Les activités du Groupe à ce jour ont porté essentiellement sur l'obtention de données épidémiologiques et de laboratoire sur la transfusion sanguine et à proposer des stratégies de sécurité transfusionnelle dans le domaine des infections transmissibles par la transfusion. Pour mener à bien ces activités de recherche, le Groupe travaille en étroite collaboration avec les Centres nationaux de transfusion sanguine (CNTS), les Centres régionaux de transfusion sanguine (CRTS), les banques de sang hospitalières (BSH) et les postes de collecte de sang. Pour les 5 premières années, quatre priorités de recherche ont été identifiées : (i) des études descriptives sur les caractéristiques des donneurs de sang et des centres de transfusion ; (ii) une estimation du risque résiduel post-transfusionnel des principales infections virales transmissibles par la transfusion ; (iii) une analyse des stratégies de sélection médicale des donneurs de sang ; et ( AbstractThere has been little blood safety research in sub-Saharan Africa, often consisting of local efforts whose findings had limited impact The "Francophone Africa Transfusion Research Network" was created in May 2007 with the objective of developing common evidence-based blood safety policies that may be adapted to each country's situation. The Group's activities to date have focused mainly on obtaining epidemiological and laboratory data on blood transfusion and on suggesting blood safety strategies, particularly in the field of TTIs. To carry out such research activities, the group works closely with the National Blood Transfusion Services (NBTS), the Regional Blood Transfusion Services (RBTS), the hospital blood banks (HBB) and collection stations. For the first 5 years, four research priorities were identified: (i) descriptive studies of the characteristics of francophone African blood donors and blood centers; (ii) estimation of the residual risk of transfusion-transmitted major viral infections; * Auteur correspondant. Author's personal copy 38 C.T. Tagny et al. / Transfusion Clinique et Biologique 21 (2014) 3...

show abstract

Transfusion safety on the African continent: an international quality control of virus testing in blood banks

Cited by 44 publications

References 37 publications

Sero-epidemiology of human immunodeficiency virus, hepatitis B and C viruses, and syphilis infections among first-time blood donors in Edéa, Cameroon

Sero-epidemiology of human immunodeficiency virus, hepatitis B and C viruses, and syphilis infections among first-time blood donors in Edéa, Cameroon

Screening for hepatitis C virus infection in a high prevalence country by an antigen/antibody combination assay versus a rapid test

The Francophone Africa Blood Transfusion Research Network: a five‐year report (2007–2012)

Contact Info

Product

Resources

About