Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses

Lin, Jie; Liu, Daofeng; Wang, Xia; Ahmed, Sajjad; Li, Mingyang; Kovinich, Nik; Sui, Shunzhao

doi:10.3390/plants10071403

Cited by 6 publications

(7 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CpNAC68 gene in wintersweet belongs to the NAM subfamily. Lin et al overexpressed the CpNAC68 gene in Arabidopsis thaliana , which enhanced the tolerance of the transgenic plants to cold, heat, salt, and osmotic stress [ 63 ]. In wheat, the NAM subfamily member TaNAC21/22 has been shown to negatively regulate wheat resistance to stripe rust [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress

Wang,

Chen,

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background The NAC TF family is widely involved in plant responses to various types of stress. Red clover (Trifolium pratense) is a high-quality legume, and the study of NAC genes in red clover has not been comprehensive. The aim of this study was to analyze the NAC gene family of red clover at the whole-genome level and explore its potential role in the Pb stress response. Results In this study, 72 TpNAC genes were identified from red clover; collinearity analysis showed that there were 5 pairs of large fragment replicators of TpNAC genes, and red clover was found to be closely related to Medicago truncatula. Interestingly, the TpNAC genes have more homologs in Arabidopsis thaliana than in soybean (Glycine max). There are many elements in the TpNAC genes promoters that respond to stress. Gene expression analysis showed that all the TpNAC genes responded to Pb stress. qRT-PCR showed that the expression levels of TpNAC29 and TpNAC42 were significantly decreased after Pb stress. Protein interaction network analysis showed that 21 TpNACs and 23 other genes participated in the interaction. In addition, the TpNAC proteins had three possible 3D structures, and the secondary structure of these proteins were mainly of other types. These results indicated that most TpNAC members were involved in the regulation of Pb stress in red clover. Conclusion These results suggest that most TpNAC members are involved in the regulation of Pb stress in red clover. TpNAC members play an important role in the response of red clover to Pb stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress

Wang,

Chen,

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Expression profiles of the gene CpBBX19 were determined under different abiotic and hormonal treatments using the six-leaf plant as the materials [ 42 ]. Drought and NaCl treatments involved the use of 50% PEG-6000 [ 43 ] and 300 mM NaCl [ 31 ] irrigation, respectively. Cold and heat stress comprised treatments at 4 °C and 42 °C [ 44 ], respectively.…”

Section: Methodsmentioning

confidence: 99%

“…CpBBX19 was fused in-frame into the pGWB551 vector with the CaMV35S promoter by Gateway recombination reactions [ 43 ] to generate the 35S:: CpBBX19 construct. Specific primers ( pGWB551 - CpBBX19 -F/R) used in the process are shown in Table S1 , and the plasmid map of the 35S:: pGWB551 vector is shown in Figure S1 .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis

Wang

Cao

et al. 2021

Genes

Self Cite

View full text Add to dashboard Cite

Zinc-finger proteins are important transcription factors in plants, responding to adversity and regulating the growth and development of plants. However, the roles of the BBX gene family of zinc-finger proteins in wintersweet (Chimonanthus praecox) have yet to be elucidated. In this study, a group IV subfamily BBX gene, CpBBX19, was identified and isolated from wintersweet. Quantitative real-time PCR (qRT-PCR) analyses revealed that CpBBX19 was expressed in all tissues and that expression was highest in cotyledons and inner petals. CpBBX19 was also expressed in all flower development stages, with the highest expression detected in early initiating bloom, followed by late initiating bloom and bloom. In addition, the expression of CpBBX19 was induced by different abiotic stress (cold, heat, NaCl, and drought) and hormone (ABA and MeJA) treatments. Heterologous expression of CpBBX19 in Arabidopsis thaliana (Arabidopsis) enhanced the tolerance of this plant to salt and drought stress as electrolyte leakage and malondialdehyde (MDA) concentrations in transgenic Arabidopsis after stress treatments were significantly lower than those in wild-type (WT) plants. In conclusion, this research demonstrated that CpBBX19 plays a role in the abiotic stress tolerance of wintersweet. These findings lay a foundation for future studies on the BBX gene family of wintersweet and enrich understanding of the molecular mechanism of stress resistance in wintersweet.

show abstract

“…The molecular mechanisms of cold tolerance and flowering of wintersweet are currently under investigation. For example, the ectopic overexpression of CpNAC68 , a transgene from wintersweet, in Arabidopsis enhanced the tolerance of transgenic plants to cold environments, yet had no effect on growth and development [ 18 ]. And, the overexpression of CpSIZ1 , a SIZ/PIAS-Type SUMO E3 ligase from wintersweet, could delay flowering time and enhance cold tolerance in Arabidopsis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

CpCAF1 from Chimonanthus praecox Promotes Flowering and Low-Temperature Tolerance When Expressed in Arabidopsis thaliana

Lv,

Xie,

Zhou

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

CCR4-associated factor I (CAF1) is a deadenylase that plays a critical role in the initial step of mRNA degradation in most eukaryotic cells, and in plant growth and development. Knowledge of CAF1 proteins in woody plants remains limited. Wintersweet (Chimonanthus praecox) is a highly ornamental woody plant. In this study, CpCAF1 was isolated from wintersweet. CpCAF1 belongs to the DEDDh (Asp-Glu-Asp-Asp-His) subfamily of the DEDD (Asp-Glu-Asp-Asp) nuclease family. The amino acid sequence showed highest similarity to the homologous gene of Arabidopsis thaliana. In transgenic Arabidopsis overexpressing CpCAF1, the timing of bolting, formation of the first rosette, and other growth stages were earlier than those of the wild-type plants. Root, lateral branch, rosette leaf, and silique growth were positively correlated with CpCAF1 expression. FLOWERING LOCUS T (FT) and SUPPRESSOROF OVEREXPRESSION OF CO 1 (SOC1) gene expression was higher while EARLY FLOWERING3 (ELF3) and FLOWERING LOCUS C (FLC) gene expression of transgenic Arabidopsis was lower than the wild type grown for 4 weeks. Plant growth and flowering occurrences were earlier in transgenic Arabidopsis overexpressing CpCAF1 than in the wild-type plants. The abundance of the CpCAF1 transcript grew steadily, and significantly exceeded the initial level under 4 °C in wintersweet after initially decreasing. After low-temperature exposure, transgenic Arabidopsis had higher proline content and stronger superoxide dismutase activity than the wild type, and the malondialdehyde level in transgenic Arabidopsis was decreased significantly by 12 h and then increased in low temperature, whereas it was directly increased in the wild type. A higher potassium ion flux in the root was detected in transgenic plants than in the wild type with potassium deficiency. The CpCAF1 promoter was a constitutive promoter that contained multiple cis-acting regulatory elements. The DRE, LTR, and MYB elements, which play important roles in response to low temperature, were identified in the CpCAF1 promoter. These findings indicate that CpCAF1 is involved in flowering and low-temperature tolerance in wintersweet, and provide a basis for future genetic and breeding research on wintersweet.

show abstract

Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses

Cited by 6 publications

References 77 publications

Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress

Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress

CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis

CpCAF1 from Chimonanthus praecox Promotes Flowering and Low-Temperature Tolerance When Expressed in Arabidopsis thaliana

Contact Info

Product

Resources

About