Transgene expression study of CXCR4 active mutants

Sharma, Menka; Afrin, Farhat; Tripathi, R. P.; Gangenahalli, Gurudutta

doi:10.4161/cam.29285

Cited by 5 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the limited number of HSCs and homing failure hinder the further development of transplantation. Promoting donor HSC homing and proliferation is one of the key measures to prevent implantation dysfunction ( 2 , 3 ).…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro

Wang

Lai

Tang

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

Recently, certain studies have demonstrated in vitro that prostaglandin E2 (PGE2) promotes human cluster of differentiation (CD)34+ cell homing. However, the sub-type receptors activated by PGE2 are unknown, as the PGE2 receptor EP1-4 subtypes (EP1-4) are expressed on the membrane of human CD34+ cells. Based on the above, the present study aimed to screen the receptor subtype activity by PGE2 to promote human CD34+ cell homing. It was observed that human CD34+ cells expressed the four PGE2 sub-receptors, particularly EP2 and 4. PGE2 increased EP2 and 4 mRNA expression significantly, while EP1 and 3 mRNA exhibited no significant alteration. PGE2, EP2 agonist (EP2A), and EP4A upregulated C-X-C chemokine receptor 4 mRNA and protein expression in human CD34+ cells, and promoted stromal cell-derived factor 1α (SDF-1α) expression in bone marrow mesenchymal stem cells (BMMSCs). These phenomena were inhibited by the associated receptor antagonists. PGE2, EP2A, and EP4A facilitated human CD34+ cell migration towards SDF-1α and BMMSCs. The results of the present study suggested that PGE2 promoted human CD34+ cell homing through EP2 and 4 receptors in vitro.

show abstract

Section: Introductionmentioning

confidence: 99%

Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro

Wang

Lai

Tang

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

show abstract

“…It is predicted that differentiated stem cells will be implantable in various tissues, which will ultimately be useful for the treatment of a wide range of diseases. Although promising, there are several issues that hinder the practicality of stem cells for regenerative therapies, including (1) low ex vivo differentiation efficiency; (2) low engraftment efficiency after transplantation; and (3) lack of the tools that would allow scientists and doctors to rapidly, easily, and precisely implant stem cells without sacrificing their viability and functionality [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene-Based Materials for Stem Cell Applications

et al. 2015

View full text Add to dashboard Cite

Although graphene and its derivatives have been proven to be suitable for several biomedical applications such as for cancer therapy and biosensing, the use of graphene for stem cell research is a relatively new area that has only recently started to be investigated. For stem cell applications, graphene has been utilized by itself or in combination with other types of materials such as nanoparticles, nanofibers, and polymer scaffolds to take advantage of the several unique properties of graphene, such as the flexibility in size, shape, hydrophilicity, as well as its excellent biocompatibility. In this review, we will highlight a number of previous studies that have investigated the potential of graphene or its derivatives for stem cell applications, with a particular focus on guiding stem cell differentiation into specific lineages (e.g., osteogenesis, neurogenesis, and oligodendrogenesis), promoting stem cell growth, stem cell delivery/transplantation, and effective monitoring of their differentiation. We hope that this review promotes and accelerates the use of graphene-based materials for regenerative therapies, especially for stem cell-based approaches to cure various incurable diseases/disorders such as neurological diseases (e.g., Alzheimer’s disease and Parkinson’s disease), stroke, spinal cord injuries, bone/cartilage defects, and cardiovascular diseases.

show abstract

Naturally occurring and synthetic constitutive-active cytokine receptors in disease and therapy

Floß

Scheller

2019

Cytokine & Growth Factor Reviews

View full text Add to dashboard Cite

Transgene expression study of CXCR4 active mutants

Cited by 5 publications

References 22 publications

Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro

Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro

Graphene-Based Materials for Stem Cell Applications

Naturally occurring and synthetic constitutive-active cytokine receptors in disease and therapy

Contact Info

Product

Resources

About