Transgenerational changes in Arabidopsis thaliana in response to UV-C, heat and cold

Rahavi, Seyed Mohammad Reza; Kovalchuk, Igor

doi:10.1016/j.bcab.2013.05.001

Cited by 6 publications

(9 citation statements)

References 39 publications

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“…7,8 It was also documented that the progeny of exposed plants had similar changes even when these plants were grown under normal conditions. 9 In previous experiments, we showed that transgenerational changes depended on the function of Dicer-like proteins DCL2 and DCL3.…”

Section: Introductionmentioning

confidence: 95%

“…However, it should be noted that in our previous work, we found that exposure of plants to a low dose of UV-C very early during development also resulted in an increase in seed size in the exposed plants. 7 Measuring F 0 seeds in order to determine a starting seed size would give the best assessment of changes caused by the increasing number of generations exposed to stress. It would also Figure 7.…”

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confidence: 99%

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Transgenerational changes in plant physiology and in transposon expression in response to UV-C stress in Arabidopsis thaliana

Migicovsky

Kovalchuk

2014

Plant Signaling & Behavior

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Abbreviations: (¡), grown under normal conditions; (C), grown under stressed conditions; C 1 , the progeny of plants grown under normal conditions in F 0 ; C 1 S 1 , the progeny of plants grown under normal conditions in F 0 and exposed to stress in F 1 ; C 2 , the progeny of plants grown under normal conditions for 2 generations; DCL, a Dicer-like protein; dpg, days post germination; ONSEN, a copia-type retrotransposon; S 1 , the progeny of plants exposed to stress in F 0 ; S 1 C 1 , the progeny of plants exposed to stress in F 0 and grown under normal conditions in F 1 ; S 2 , the progeny of plants exposed to stress for 2 generations; TEs, transposable elements; TSI, transcriptionally silent information; UV-C, ultraviolet radiation C.Stress has a negative impact on crop yield by altering a gain in biomass and affecting seed set. Recent reports suggest that exposure to stress also influences the response of the progeny. In this paper, we analyzed seed size, leaf size, bolting time and transposon expression in 2 consecutive generations of Arabidopsis thaliana plants exposed to moderate UV-C stress. Since previous reports suggested a potential role of Dicer-like (DCL) proteins in the establishment of transgenerational response, we used dcl2, dcl3 and dcl4 mutants in parallel with wild-type plants. These studies revealed that leaf number decreased in the progeny of UV-C stressed plants, and bolting occurred later. Transposons were also re-activated in the progeny of stressed plants. Changes in the dcl mutants were less prominent than in wild-type plants. DCL2 and DCL3 appeared to be more important in the transgenerational stress memory than DCL4 because transgenerational changes were less profound in the dcl2 and dcl3 mutants.

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Section: Introductionmentioning

confidence: 95%

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confidence: 99%

Transgenerational changes in plant physiology and in transposon expression in response to UV-C stress in Arabidopsis thaliana

Migicovsky

Kovalchuk

2014

Plant Signaling & Behavior

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“…Our study demonstrates that changes in the temporal patterns of heat stress can carry over to the next generation in A. thaliana , and that there is substantial genotypic variation in the magnitude and direction of these transgenerational effects. Reza Rahavi and Kovalchuk (2013) also manipulated heat stress timing in A. thaliana and found transgenerational effects: offspring from parents that were stressed at Day 7 after germination increased stem length and fresh weight compared to control plants after renewed heat stress, while offspring from parents that were stressed at Day 21 decreased stem length. Whereas they investigated a single genotype, our study reveals significant genotypic variation in transgenerational effects for some traits.…”

Section: Discussionmentioning

confidence: 99%

“…It is not surprising that heat stress effects differed between these plants. However, all arguments so far, as well as the empirical studies mentioned above, are about within-generation responses to heat stress, whereas in our study we examined transgenerational effects of the timing of heat stress (see also Reza Rahavi and Kovalchuk 2013 ). Thus, signalling and developmental regulation alone cannot explain our results, and there must be additional, so far unknown, physiological ( Herman and Sultan 2011 ) and/or epigenetic ( Whittle et al 2009 ; Rasmann et al 2012 ), mechanisms involved.…”

Section: Discussionmentioning

confidence: 99%

“…To date, only few studies have examined plant responses to changes in environmental variability, or genetic variation therein ( Parepa et al 2013 ; Scheepens et al 2018 ), specifically with respect to the timing ( Stone and Nicolas 1995 , 1996 ; Prasad et al 1999 ; Wang et al 2016 ) or frequency ( Walter et al 2009 ) of stress. To our knowledge, only one previous study tested for transgenerational effects of stress timing ( Reza Rahavi and Kovalchuk 2013 ) and none tested transgenerational effects of stress frequency.…”

Section: Introductionmentioning

confidence: 99%

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Transgenerational effects of temperature fluctuations inArabidopsis thaliana

Deng

Bossdorf

2021

AoB PLANTS

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Plant stress responses can extend into the following generations, a phenomenon called transgenerational effects. Heat stress, in particular, is known to affect plant offspring, but we do not know to what extent these effects depend on the temporal patterns of the stress, and whether transgenerational responses are adaptive and genetically variable within species. To address these questions, we carried out a two-generation experiment with nine Arabidopsis thaliana genotypes. We subjected the plants to heat stress regimes that varied in timing and frequency, but not in mean temperature, and we then grew the offspring of these plants under controlled conditions as well as under renewed heat stress. The stress treatments significantly carried over to the offspring generation, with timing having stronger effects on plant phenotypes than stress frequency. However, there was no evidence that transgenerational effects were adaptive. The magnitudes of transgenerational effects differed substantially among genotypes, and for some traits the strength of plant responses was significantly associated with the climatic variability at the sites of origin. In summary, timing of heat stress not only directly affects plants, but it can also cause transgenerational effects on offspring phenotypes. Genetic variation in transgenerational effects, as well as correlations between transgenerational effects and climatic variability, indicate that transgenerational effects can evolve, and have probably already done so in the past.

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Time dynamics of stress legacy in clonal transgenerational effects: A case study on Trifolium repens

Quan

Münzbergová

Latzel

2022

Ecology and Evolution

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Stress can be remembered by plants in a form of stress legacy that can alter future phenotypes of previously stressed plants and even phenotypes of their offspring. DNA methylation belongs among the mechanisms mediating the stress legacy. It is however not known for how long the stress legacy is carried by plants. If the legacy is long‐lasting, it can become maladaptive in situations when parental–offspring environment do not match. We investigated for how long after the last exposure of a parental plant to drought can the phenotype of its clonal offspring be altered. We grew parental plants of three genotypes of Trifolium repens for five months either in control conditions or in control conditions that were interrupted with intense drought periods applied for two months in four different time slots. We also treated half of the parental plants with a demethylating agent (5‐azacytidine, 5‐azaC) to test for the potential role of DNA methylation in the stress memory. Then, we transplanted parental cuttings (ramets) individually to control environment and allowed them to produce offspring ramets for two months. The drought stress experienced by parents affected phenotypes of offspring ramets. The stress legacy resulted in enhanced number of offspring ramets originating from plants that experienced drought stress even 56 days before their transplantation to the control environment. 5‐azaC altered transgenerational effects on offspring ramets. We confirmed that drought stress can trigger transgenerational effects in T . repens that is very likely mediated by DNA methylation. Most importantly, the stress legacy in parental plants persisted for at least 8 weeks suggesting that the stress legacy can persist in a clonal plant Trifolium repens for relatively long period. We suggest that the stress legacy should be considered in future ecological studies on clonal plants.

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Transgenerational changes in Arabidopsis thaliana in response to UV-C, heat and cold

Cited by 6 publications

References 39 publications

Transgenerational changes in plant physiology and in transposon expression in response to UV-C stress in Arabidopsis thaliana

Transgenerational changes in plant physiology and in transposon expression in response to UV-C stress in Arabidopsis thaliana

Transgenerational effects of temperature fluctuations inArabidopsis thaliana

Time dynamics of stress legacy in clonal transgenerational effects: A case study on Trifolium repens

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