Transgenerational Effects of Copper on a Freshwater Gastropod, Planorbella pilsbryi

Osborne, Rebecca K.; Gillis, Patricia L.; Prosser, Ryan S.

doi:10.31931/fmbc.v22i2.2020.42-54

Cited by 11 publications

(8 citation statements)

References 52 publications

(62 reference statements)

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“…Copper (Cu) is one of the most widely used metals in industrial processes and agriculture (Gledhill et al, 1997; Nriagu, 1996). Although Cu is an essential micronutrient for both prokaryotes and eukaryotes (Zhu et al, 2014), high concentrations of Cu in aquatic systems can have toxic effects that can alter individual physiology, increase mutation rates and lead to genetic erosion, potentially reducing population fitness (De Schamphelaere et al, 2007; Heugens et al, 2001; Long et al, 2004; Osborne et al, 2020; Poynton et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genotype diversity promotes the persistence of Daphnia populations exposed to severe copper stress

Loria

Cristescu

Gonzalez

2022

J of Evolutionary Biology

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Section: Introductionmentioning

confidence: 99%

Genotype diversity promotes the persistence of Daphnia populations exposed to severe copper stress

Loria

Cristescu

Gonzalez

2022

J of Evolutionary Biology

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“…Extracts from two species of freshwater snails, P. pilsbryi (file ramshorn snail) and C. chinensis (Chinese mystery snail) and, one species of terrestrial snail, C. nemoralis (brown-lipped snail) were prepared. P. pilsbryi were obtained from a continuous culture that is maintained in the School of Environmental Sciences at the University of Guelph 25 . C. chinensis were collected from a site on the Speed River downstream of the City of Guelph.…”

Section: Methodsmentioning

confidence: 99%

Differentiated extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans

Gutierrez-Gongora

Raouf-Alkadhimi

Prosser

et al. 2023

Sci Rep

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The human fungal pathogen, Cryptococcus neoformans, is responsible for deadly infections among immunocompromised individuals with the evolution of antifungal resistance driving the solution to discover new compounds that inhibit fungal virulence factors rather than kill the pathogen. Recently, exploration into natural sources (e.g., plants, invertebrates, microbes) of antifungal agents has garnered attention by integrating a One Health approach for new compound discovery. Here, we explore extracts from three mollusk species (freshwater and terrestrial) and evaluate effects against the growth and virulence factor production (i.e., thermotolerance, melanin, capsule, and biofilm) in C. neoformans. We demonstrate that clarified extracts of Planorbella pilsbryi have a fungicidal effect on cryptococcal cells comparable to fluconazole. Similarly, all extracts of Cipangopaludina chinensis affect cryptococcal thermotolerance and impair biofilm and capsule production, with clarified extracts of Cepaea nemoralis also conveying the latter effect. Next, inhibitory activity of extracts against peptidases related to specific virulence factors, combined with stress assays and quantitative proteomics, defined distinct proteome signatures and proposed proteins driving the observed anti-virulence properties. Overall, this work highlights the potential of compounds derived from natural sources to inhibit virulence factor production in a clinically important fungal pathogen.

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“…Proteins were extracted from two species of freshwater snails, P. pilsbryi ( le ramshorn snail) and C. chinensis (Chinese mystery snail) and, one species of terrestrial snail, C. nemoralis (brown-lipped snail). P. pilsbryi were obtained from a continuous culture that is maintained in the School of Environmental Sciences at the University of Guelph 25 . C. chinensis were collected from a site on the Speed River downstream of the City of Guelph.…”

Section: Protein Extractionmentioning

confidence: 99%

Differentiated extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans

Gutierrez-Gongora

Raouf-Alkadhimi

Prosser

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The human fungal pathogen, Cryptococcus neoformans, is responsible for deadly infections among immunocompromised individuals with the evolution of antifungal resistance driving the need for discovery of new compounds that inhibit fungal virulence factors, rather than kill the pathogen. Recently, exploration into natural sources (e.g., plants, invertebrates, microbes) of antifungal agents has garnered attention by integrating a One Health approach for new compound discovery. Here, we explore extracts from three mollusk species (freshwater and terrestrial) and evaluate effects against the growth and virulence factor production (i.e., thermotolerance, melanin, capsule, and biofilm) in C. neoformans. We demonstrate that clarified extracts of Planorbella pilsbryi have a fungicidal effect on cryptococcal cells in a comparable way to fluconazole. Similarly, all extracts of Cipangopaludina chinensis not only affect cryptococcal thermotolerance but also impair biofilm and capsule production with clarified extracts of Cepaea nemoralis also conveying the latter effect. Next, inhibitory activity of extracts against peptidases related to these virulence factors combined with quantitative proteomics defined distinct proteome signatures and proposed proteins driving the observed anti-virulence properties. Overall, this work highlights the potential of compounds derived from natural sources to inhibit virulence factor production in a clinically important fungal pathogen.

show abstract

Transgenerational Effects of Copper on a Freshwater Gastropod, Planorbella pilsbryi

Cited by 11 publications

References 52 publications

Genotype diversity promotes the persistence of Daphnia populations exposed to severe copper stress

Genotype diversity promotes the persistence of Daphnia populations exposed to severe copper stress

Differentiated extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans

Differentiated extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans

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