Transgenic and Knockout Mice

SummaryT his is a report on the potential in¯uence of circadian changes and laborat ory routines on some immune param eters (thymic and splenic weights, the numbers of bone marrow, peripheral blood, and peritoneal leukocytes) in: (1 ) males of C57BL =6J, Balb =c, and CB6 F1 mice kept under identical laborat ory conditions; (2 ) males of CB6 mice kept under the same laboratory conditions, except for opposite light =dark regimes, either light =dark (LD ) or dark =light (DL). All the animals were purchased from the sam e supplier and adapted for 4±5 weeks to strictly controlled housing conditions. Some param eters were similar at certain time points but statistically signi®cantly different at others due to strain-speci®c daily variations. In order to make the interstrain comparisons more reliable, the data collected around the day=night cycle were pooled for calculations of mean values. Several immune param eters of CB6 mice kept under DL conditions were signi®cantly different than those in mice under the conventional LD conditions. In conclusion, the extrapolati on of results (especially in the ®eld of neuroimmunology) to other strains (or species) should be done with great caution; and all interstrain (interspeci®c) comparisons, especially those from various laborat ories, should always be related to speci®c time points and laboratory conditions.

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Transgenic and Knockout Mice

Cited by 2 publications

References 26 publications

Genetically Modified Animals

Genetically Modified Animals

Strain differences in some immune parameters can be obscured by circadian variations and laboratory routines: studies of male C57BL/6J, Balb/c and CB6 F1 mice

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