Transgenic aspen and birch trees for Russian plantation forests

Shestibratov, Konstantin A.; Lebedev, V. T.; Podrezov, Alexey; Salmova, Margarita

doi:10.1186/1753-6561-5-s7-p124

Cited by 12 publications

(7 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These plants are promising for further studies, in particular to field testing. In addition, the bar gene could be used for retransformation of the transgenic woody plants that have been obtained in our laboratory and have already demonstrated valuable traits, such as increased productivity and modification of the composition of wood [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Testing Transgenic Aspen Plants with bar Gene for Herbicide Resistance under Semi-natural Conditions

et al. 2016

View full text Add to dashboard Cite

Obtaining herbicide resistant plants is an important task in the genetic engineering of forest trees. Transgenic European aspen plants (Populus tremula L.) expressing the bar gene for phosphinothricin resistance have been produced using Agrobacterium tumefaciens-mediated transformation. Successful genetic transformation was confirmed by PCR analysis for thirteen lines derived from two elite genotypes. In 2014–2015, six lines were evaluated for resistance to herbicide treatment under semi-natural conditions. All selected transgenic lines were resistant to the herbicide Basta at doses equivalent to 10 l/ha (twofold normal field dosage) whereas the control plants died at 2.5 l/ha. Foliar NH4-N concentrations in transgenic plants did not change after treatment. Extremely low temperatures in the third ten-day period of October 2014 revealed differences in freeze tolerance between the lines obtained from Pt of f2 aspen genotypes. Stable expression of the bar gene after overwintering outdoors was confirmed by RT-PCR. On the basis of the tests, four transgenic aspen lines were selected. The bar gene could be used for retransformation of transgenic forest trees expressing valuable traits, such as increased productivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Testing Transgenic Aspen Plants with bar Gene for Herbicide Resistance under Semi-natural Conditions

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Leaves from plants in vitro were used as explants and were transformed according to the method of Lebedev et al [22]. The transgenic status of the obtained kanamycin-tolerant transformants was confirmed by PCR and RT-PCR [23].…”

Section: Methodsmentioning

confidence: 99%

Effect of Phosphinothricin on Transgenic Downy Birch (Betula pubescens Ehrh.) Containing bar or GS1 Genes

Lebedev

Krutovsky

Shestibratov

2019

Forests

View full text Add to dashboard Cite

Weeds are a big problem in agriculture and forestry, and herbicides are the main tools to control them. Phosphinotricin (ammonium glufosinate, PPT) is one of the most effective non-selective herbicides, to which weeds hardly gain resistance, but the reasons for its effect and toxicity to plants are still unclear, and especially, it is little studied in trees, including transgenic ones. We studied the physiological responses of downy birch (Betula pubescens Ehrh.) containing the herbicide resistance bar gene or the cytosol glutamine synthetase GS1 gene (the target enzyme of the herbicide) to PPT-based Basta herbicide treatment in various doses under open-air conditions during two years. Birch saplings with the bar gene were resistant to a double field dose (10 L/ha), but the expression of the GS1 gene only slightly increased resistance compared to the control. Herbicide treatment increased the ammonium level in leaf tissue by 3–8 times, but this, apparently, was not the main cause of plant death. Among leaf pigments, chlorophyll B was the most resistant to PPT, and carotenoids were the most sensitive. Responses of birch trees with the GS1 gene (accumulation of ammonium, pigment content, and dehydration) during treatment with a low dose of herbicide were less pronounced than in control plants. One-year-old control and transgenic plants with the GS gene died after 2.5 L/ha treatment, and two-year-old plants lost foliage after such treatment but remained alive and developed buds four weeks after treatment. Herbicide treatment of plants with the bar gene did not cause significant deviations in height (first year) or the accumulation of aboveground biomass (second year). The obtained results improve our understanding of the effect of PPT on woody plants and can be used both to clarify mechanisms of herbicide action and in plantation forestry.

show abstract

“…In order to increase productivity of forest plantations the gene of cytosolic GS from Pinus sylvestris was transferred into the genome of Betula [12]. Transgenic plants were tested for growth rate enhancement in the greenhouse [13] and resistance to phosphinothricin treatment [14]. As an extension of the evaluation of these plants, in the present work we estimated the effect of the pine GS1a gene expression on the growth and leaf pigment levels of two birch species ( Betula pubescens and B. pendula ) grown under three different nitrogen levels in outdoor conditions.…”

Section: Introductionmentioning

confidence: 99%

Influence of Nitrogen Availability on Growth of Two Transgenic Birch Species Carrying the Pine GS1a Gene

Lebedev

P.Kovalenko

Shestibratov

2017

Plants

Self Cite

View full text Add to dashboard Cite

An alternative way to increase plant productivity through the use of nitrogen fertilizers is to improve the efficiency of nitrogen utilization via genetic engineering. The effects of overexpression of pine glutamine synthetase (GS) gene and nitrogen availability on growth and leaf pigment levels of two Betula species were studied. Untransformed and transgenic plants of downy birch (B. pubescens) and silver birch (B. pendula) were grown under open-air conditions at three nitrogen regimes (0, 1, or 10 mM) for one growing season. The transfer of the GS1a gene led to a significant increase in the height of only two transgenic lines of nine B. pubescens, but three of five B. pendula transgenic lines were higher than the controls. In general, nitrogen supply reduced the positive effect of the GS gene on the growth of transgenic birch plants. No differences in leaf pigment levels between control and transgenic plants were found. Nitrogen fertilization increased leaf chlorophyll content in untransformed plants but its effect on most of the transgenic lines was insignificant. The results suggest that birch plants carrying the GS gene use nitrogen more efficiently, especially when growing in nitrogen deficient soil. Transgenic lines were less responsive to nitrogen supply in comparison to wild-type plants.

show abstract

Transgenic aspen and birch trees for Russian plantation forests

Cited by 12 publications

References 3 publications

Testing Transgenic Aspen Plants with bar Gene for Herbicide Resistance under Semi-natural Conditions

Testing Transgenic Aspen Plants with bar Gene for Herbicide Resistance under Semi-natural Conditions

Effect of Phosphinothricin on Transgenic Downy Birch (Betula pubescens Ehrh.) Containing bar or GS1 Genes

Influence of Nitrogen Availability on Growth of Two Transgenic Birch Species Carrying the Pine GS1a Gene

Contact Info

Product

Resources

About