Transgenic chickpea (Cicer arietinum L.) harbouring AtDREB1a are physiologically better adapted to water deficit

Patel

et al. 2021

IJMS

Self Cite

120

Metabolic regulation is the key mechanism implicated in plants maintaining cell osmotic potential under drought stress. Understanding drought stress tolerance in plants will have a significant impact on food security in the face of increasingly harsh climatic conditions. Plant primary and secondary metabolites and metabolic genes are key factors in drought tolerance through their involvement in diverse metabolic pathways. Physio-biochemical and molecular strategies involved in plant tolerance mechanisms could be exploited to increase plant survival under drought stress. This review summarizes the most updated findings on primary and secondary metabolites involved in drought stress. We also examine the application of useful metabolic genes and their molecular responses to drought tolerance in plants and discuss possible strategies to help plants to counteract unfavorable drought periods.

Section: Genetic Engineering Of Metabolic Genes To Improve Drought Tolerance In Plantsmentioning

confidence: 99%

Metabolomics and Molecular Approaches Reveal Drought Stress Tolerance in Plants

Patel

et al. 2021

IJMS

Self Cite

120

“…The transgenic chickpeas exhibited higher relative water content, longer chlorophyll retention and higher osmotic adjustment under severe drought stress, and the seed yield was significantly higher than the control. Under well water conditions, there was no significant difference in seed yield between the transgenic and WT lines (Das et al, 2021). GmTDN 1, a gene encoding a DREB‐like transcription factor in soybean, was proved to be a useful gene that confers ADR (Chen et al, 2009).…”

Section: Concept Of Adrmentioning

confidence: 99%

Potential breeding target genes for enhancing agronomic drought resistance: A yield‐survival balance perspective

Liu,

Ning,

Chen

et al. 2023

Plant Breeding

Amidst global climate warming, the urgency to enhance crop drought resistance has reached unprecedented levels. However, the achievement of superior drought‐resistant crop varieties, despite substantial research investments, remains constrained. This limited success in transitioning from the laboratory to the field can be partly attributed to the disparity between evaluating biological and agronomic drought resistance (ADR). ADR places emphasis on minimizing yield losses during drought conditions and maintaining robust performance under normal circumstances. Here, we present a comprehensive overview of ADR genes reported during the past decades, categorized based on their yield performance under both drought and standard growth conditions. We highlight 23 genes from grain and legume crops, providing insight into their working mechanisms. Particularly, we delve into their efficacy in improving yields predominantly through transgenic approaches in field conditions. Furthermore, we briefly touch upon the adoption of emerging phenomics technologies, which can streamline the discovery and application of ADR genes. This review is poised to serve the breeding community, aiding in the selection of appropriate target genes to augment crop drought resistance.

“…Therefore, it is a targeted gene-based functional genomics tool that offers valuable information to understand the regulatory mechanisms underlying abiotic stress tolerance in plants [ 154 ]. In this context, some efficient transformation protocols have been developed and applied in legume crops, including chickpea [ 155 , 156 , 157 ].…”

Section: Breeding Approaches In Chickpeamentioning

confidence: 99%

“…For example, Bhatnagar-Mathur et al [ 156 ] used transgenic technology for the introduction of an osmoregulatory gene P5CSF129A under the CaMV35S promoter, which improved drought stress tolerance in chickpea by enhancing proline accumulation. Similarly, the expression of AtDREB1A under the drought inducible Rd29A promoter influenced the mechanisms underlying water uptake, stomatal response, transpiration efficiency, and rooting architecture and enhanced drought tolerance in transgenic chickpea lines [ 157 , 158 ]. On the other hand, the silencing of an HD-Zip I gene, CaHDZ12, resulted in increased sensitivity to salt and drought stresses in chickpea [ 159 ].…”

Section: Breeding Approaches In Chickpeamentioning

confidence: 99%

A Comprehensive Review on Chickpea (Cicer arietinum L.) Breeding for Abiotic Stress Tolerance and Climate Change Resilience

Arriagada

Cacciuttolo

Cabeza

et al. 2022

IJMS

Chickpea is one of the most important pulse crops worldwide, being an excellent source of protein. It is grown under rain-fed conditions averaging yields of 1 t/ha, far from its potential of 6 t/ha under optimum conditions. The combined effects of heat, cold, drought, and salinity affect species productivity. In this regard, several physiological, biochemical, and molecular mechanisms are reviewed to confer tolerance to abiotic stress. A large collection of nearly 100,000 chickpea accessions is the basis of breeding programs, and important advances have been achieved through conventional breeding, such as germplasm introduction, gene/allele introgression, and mutagenesis. In parallel, advances in molecular biology and high-throughput sequencing have allowed the development of specific molecular markers for the genus Cicer, facilitating marker-assisted selection for yield components and abiotic tolerance. Further, transcriptomics, proteomics, and metabolomics have permitted the identification of specific genes, proteins, and metabolites associated with tolerance to abiotic stress of chickpea. Furthermore, some promising results have been obtained in studies with transgenic plants and with the use of gene editing to obtain drought-tolerant chickpea. Finally, we propose some future lines of research that may be useful to obtain chickpea genotypes tolerant to abiotic stress in a scenario of climate change.