Transgenic cotton expressing Mpp51Aa2 does not adversely impact beneficial non-target hemiptera in the field

Asiimwe, P.; Brown, C.R.; Ellsworth, P.C.; Reisig, D.D.; Bertho, L.; Jiang, C.; Schapaugh, A.; Head, G.; Burzio, L.

doi:10.1016/j.cropro.2023.106384

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…Likewise, Graham and Stewart [21] and Graham et al [20] found that ThryvOn had fewer tarnished plant bug adults than the non-traited control, suggesting that the trait had a repellency effect on the pest, with adults preferentially feeding on the non-traited plants. This same result was reported in cotton fleahoppers, where the adult populations in ThryvOn were significantly lower than in the non-traited [28]. In contrast, the adult cotton fleahopper populations at the end of the squaring period in each year of our study suggest the singular Bt trait effect did not significantly suppress adult cotton fleahopper populations.…”

Section: Discussionsupporting

confidence: 89%

“…This observation aligns with Graham and Stewart [21], who reported a delay in cotton fleahopper reinfestation when ThryvOn cotton was over-sprayed with insecticide, potentially reducing the number of insecticide applications required to manage populations. Similar to the results of Asiimwe et al [28] populations of nymphs, small or large, did not consistently show significant differences based on trait. However, differences were observed in CIDs for both large nymphs and adults in this study.…”

Section: Discussionsupporting

confidence: 88%

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Field Evaluation of Cotton Expressing Mpp51Aa2 as a Management Tool for Cotton Fleahoppers, Pseudatomoscelis seriatus (Reuter)

Arthur,

Suh,

McKnight

et al. 2023

Toxins

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The cotton fleahopper (Pseudatomoscelis seriatus Reuter) is considered a highly economically damaging pest of cotton (Gossypium hirsutum L.) in Texas and Oklahoma. Current control methods rely heavily on the use of foliar-applied chemical insecticides, but considering the cost of insecticides and the critical timeliness of applications, chemical control methods are often not optimized to reduce potential yield losses from this pest. The Bacillus thuringiensis (Bt) Mpp51Aa2 (formerly Cry51Aa2.834_16) protein has proven effective against thrips and plant bugs with piercing and sucking feeding behaviors, but the impact of this toxin on cotton fleahoppers has not been investigated. To evaluate the Mpp51Aa2 trait effectiveness towards the cotton fleahopper, field trials were conducted in 2019, 2020, and 2021, comparing a cotton cultivar containing the Mpp51Aa2 trait to a non-traited isoline cultivar under insecticide-treated and untreated conditions. Populations of cotton fleahopper nymphs and adults were estimated weekly by visually inspecting cotton terminals. Square retention was also assessed during the first week of bloom to provide some insight on how the Bt trait may influence yield. While cotton fleahopper population differences between the traited and non-traited plants were not consistently noted during the pre-bloom squaring period, there was a consistent increase in square retention in cotton expressing Mpp51Aa2 relative to non-traited cotton. Additionally, cotton expressing Mpp51Aa2 offered similar square protection relative to non-traited cotton treated with insecticides for the cotton fleahopper. These findings indicate that the Mpp51Aa2 protein should provide benefits of delayed nymphal growth, population suppression, and increased square retention.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 88%

Field Evaluation of Cotton Expressing Mpp51Aa2 as a Management Tool for Cotton Fleahoppers, Pseudatomoscelis seriatus (Reuter)

Arthur,

Suh,

McKnight

et al. 2023

Toxins

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“…Bachman et al [25] observed a substantial 19-fold decrease in tarnished plant bug large nymphs on cotton expressing Cry51Aa2 relative to a gene-lacking variety. Although square retention or evidence of feeding was not reported, Bachman et al [25] and Asiimwe et al [37] did observe significantly higher numbers of adult cotton fleahoppers on gene-lacking cotton relative to those found in the cotton expressing the Mpp51Aa2 protein.…”

Section: Discussionmentioning

confidence: 94%

“…As shown by Arthur et al [35], cotton expressing the Mpp51Aa2 protein suffered a significant square loss when exposed to high populations of cotton fleahoppers. Previous research has consistently concluded that ThryvOn cotton can deter the feeding of various insect pests of cotton, including tobacco thrips (Frankliniella fusca Hinds), western flower thrips (Frankliniella occidentalis Pergande), onion thrips (Thrips tabaci Lindeman), tarnished plant bugs, western tarnished plant bugs, and cotton fleahoppers [18,36,37]. In this choice feeding assay the feeding preference of cotton fleahoppers would have been difficult to monitor as the insects could move from plant to plant.…”

Section: Discussionmentioning

confidence: 99%

Choice and No-Choice Feeding Assays of Cotton Fleahoppers (Pseudatomoscelis seriatus) on Cotton Expressing the Mpp51Aa2 Protein

Arthur,

Suh,

McKnight

et al. 2023

Agronomy

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In Texas, the cotton fleahopper (Pseudatomoscelis seriatus (Reuter)) is considered a highly economically damaging pest of cotton (Gossypium hirsutum L.). Current control methods rely heavily on foliar chemical insecticides throughout the growing season. Considering the cost of insecticides and the critical timeliness of their application, chemical control methods are often not optimized to reduce potential yield losses. The Mpp51Aa2.834_16 gene in cotton (ThryvOn) has shown effectiveness against thrips and several piercing and sucking mirid insect pests, suggesting it has the potential to mitigate yield losses caused by the cotton fleahopper. Choice and no-choice caged feeding assays were conducted to assess the impact of cotton fleahoppers on ThryvOn cotton square retention under controlled laboratory conditions. In the choice assay, feeding by cotton fleahoppers significantly reduced square retention in the gene-lacking cotton to 46%, while the ThryvOn cotton retained 60% of the squares. In the no-choice assay, cotton fleahopper nymph feeding significantly reduced square retention in the cotton not expressing Mpp51Aa2 to 61%, whereas the ThryvOn cotton was unaffected. Based on the differences in square retention observed in both the choice and no-choice feeding assays, our findings indicate that the Mpp51Aa2 protein influences cotton fleahopper feeding preferences and the susceptibility of cotton plants to damage caused by cotton fleahoppers. Our study offers confirmation of the activity of ThryvOn on cotton fleahoppers observed in the field. The ThryvOn trait’s activity towards cotton fleahoppers is consistent with that found for other mirid pests in cotton.

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“…The results of this study thus confirm the outcome of the environmental risk assessment (summarized in USDA-APHIS 2020 ) that predatory bug populations are unlikely to suffer harm from MON 88702 cotton in the field, because it is unlikely that their diet consists predominantly of spider mites and other prey types with similarly high Bt protein concentrations. In fact, arthropod communities have been studied in field experiments with MON 88702 cotton over three years at 5–6 sites in various cotton growing regions of the USA (Asiimwe et al 2023 ). No significant differences in abundance of Orius spp.…”

Section: Discussionmentioning

confidence: 99%

Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus

Meissle

2024

Transgenic Res

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Genetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to further characterize risks to this important group of non-target organisms. In this work, exclusive feeding of frozen spider mites (Tetranychus urticae, Acari: Tetranychidae) from Bt cotton confirmed adverse effects on longevity and fecundity of O. majusculus adults. Alternate feeding of Bt protein-containing spider mites and Bt-free Ephestia kuehniella (Lepidoptera: Pyralidae) eggs mitigated effects on longevity, but not on fecundity. When living larvae of Spodoptera littoralis (Lepidoptera: Noctuidae) from Bt cotton were fed to the predators, however, no effects on longevity and reproduction of female O. majusculus were observed, despite the fact that Bt protein concentrations in larvae were almost as high as concentrations in spider mites. When a diverse mix of prey species with various Bt protein concentrations is consumed in the field, it is unlikely that exposure of Orius spp. to Mpp51Aa2 is high enough to exert adverse effects on predator populations. MON 88702 cotton may thus be a valuable tool for integrated management of sucking pests.

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Transgenic cotton expressing Mpp51Aa2 does not adversely impact beneficial non-target hemiptera in the field

Cited by 7 publications

References 39 publications

Field Evaluation of Cotton Expressing Mpp51Aa2 as a Management Tool for Cotton Fleahoppers, Pseudatomoscelis seriatus (Reuter)

Field Evaluation of Cotton Expressing Mpp51Aa2 as a Management Tool for Cotton Fleahoppers, Pseudatomoscelis seriatus (Reuter)

Choice and No-Choice Feeding Assays of Cotton Fleahoppers (Pseudatomoscelis seriatus) on Cotton Expressing the Mpp51Aa2 Protein

Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus

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