Transgenic cytoplasmic incompatibility persists across age and temperature variation in Drosophila melanogaster

Ritchie, Isabella T.; Needles, Kelly T.; Leigh, Brittany A.; Kaur, Rupinder; Bordenstein, Seth R.

doi:10.1016/j.isci.2022.105327

Cited by 3 publications

(2 citation statements)

References 86 publications

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“…Drive component efficiency may vary according to factors like climate and insect age. It may have a substantial impact on drive performance [ 66 , 67 ]. We consider drive component efficiency as well for models where cifA and cifB are in the same genetic construct.…”

Section: Methodsmentioning

confidence: 99%

Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study

Champer

2023

PLoS Genet

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Wolbachia are maternally-inherited bacteria, which can spread rapidly in populations by manipulating reproduction. cifA and cifB are genes found in Wolbachia phage that are responsible for cytoplasmic incompatibility, the most common type of Wolbachia reproductive interference. In this phenomenon, no viable offspring are produced when a male with both cifA and cifB (or just cifB in some systems) mates with a female lacking cifA. Utilizing this feature, we propose new types of toxin-antidote gene drives that can be constructed with only these two genes in an insect genome, instead of the whole Wolbachia bacteria. By using both mathematical and simulation models, we found that a drive containing cifA and cifB together creates a confined drive with a moderate to high introduction threshold. When introduced separately, they act as a self-limiting drive. We observed that the performance of these drives is substantially influenced by various ecological parameters and drive characteristics. Extending our models to continuous space, we found that the drive individual release distribution has a critical impact on drive persistence. Our results suggest that these new types of drives based on Wolbachia transgenes are safe and flexible candidates for genetic modification of populations.

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Section: Methodsmentioning

confidence: 99%

Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study

Champer

2023

PLoS Genet

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“…aegypti mosquito hosts, Wolbachia induce strong CI [23,25,34], unlike in the natural host D. melanogaster where CI strength varies [35][36][37][38][39][40][41][42][43]. Variation in CI strength can depend on various factors such as Wolbachia levels in testes [44], host age [35][36][37][38][39][40], temperature [40][41][42][43], maternal symbiont densities [45], and relevant to this study, different Wolbachia genotypes interacting in their natural versus artificial host systems. For instance, in D. simulans, wRi induces nearly complete CI, while wNo and wHa strains induce lower levels of CI [46].…”

Section: Introductionmentioning

confidence: 99%

The mechanism of cytoplasmic incompatibility is conserved in Wolbachia-infected Aedes aegypti mosquitoes deployed for arbovirus control

Kaur,

Meier,

McGraw

et al. 2024

PLoS Biol

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The rising interest and success in deploying inherited microorganisms and cytoplasmic incompatibility (CI) for vector control strategies necessitate an explanation of the CI mechanism. Wolbachia-induced CI manifests in the form of embryonic lethality when sperm from Wolbachia-bearing testes fertilize eggs from uninfected females. Embryos from infected females however survive to sustain the maternally inherited symbiont. Previously in Drosophila melanogaster flies, we demonstrated that CI modifies chromatin integrity in developing sperm to bestow the embryonic lethality. Here, we validate these findings using wMel-transinfected Aedes aegypti mosquitoes released to control vector-borne diseases. Once again, the prophage WO CI proteins CifA and CifB target male gametic nuclei to modify chromatin integrity via an aberrant histone-to-protamine transition. Cifs are not detected in the embryo, and thus elicit CI via the nucleoprotein modifications established pre-fertilization. The rescue protein CifA in oogenesis localizes to stem cell, nurse cell, and oocyte nuclei, as well as embryonic DNA during embryogenesis. Discovery of the nuclear targeting Cifs and altered histone-to-protamine transition in both Aedes aegypti mosquitoes and D. melanogaster flies affirms the Host Modification Model of CI is conserved across these host species. The study also newly uncovers the cell biology of Cif proteins in the ovaries, CifA localization in the embryos, and an impaired histone-to-protamine transition during spermiogenesis of any mosquito species. Overall, these sperm modification findings may enable future optimization of CI efficacy in vectors or pests that are refractory to Wolbachia transinfections.

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The impact of environmental and host factors on wolbachia density and efficacy as a biological tool

Padde,

Lu,

Long

et al. 2023

Decoding Infection and Transmission

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Transgenic cytoplasmic incompatibility persists across age and temperature variation in Drosophila melanogaster

Cited by 3 publications

References 86 publications

Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study

Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study

The mechanism of cytoplasmic incompatibility is conserved in Wolbachia-infected Aedes aegypti mosquitoes deployed for arbovirus control

The impact of environmental and host factors on wolbachia density and efficacy as a biological tool

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