Transgenic Eimeria parasite: A potential control strategy for chicken coccidiosis

Fatoba, Abiodun J.; Adeleke, Matthew Adekunle

doi:10.1016/j.actatropica.2020.105417

Cited by 21 publications

(20 citation statements)

References 56 publications

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“…Poultry production losses caused by avian necrotic enteritis (NE) and parasitic diseases, such as coccidiosis, have become a global challenge for the poultry industry ( 6 , 7 ). Since the ban on antibiotics in animal feed, the high prevalence of NE and coccidiosis has become a major cause of mortality in broilers ( 8 – 10 ) ( Figure 2 ).…”

Section: Lessons From the Gut Health Impairment In Broilersmentioning

confidence: 99%

“…The main cause of coccidiosis outbreaks is the protozoan Eimeria species, and the infection route is through fecal–oral transmission ( 2 , 9 ). The Eimeria species increase their environmental survival through their ability as oocysts and their drug resistance ( 9 , 10 ). Eimeria induces plasma protein leakage by damaging epithelial cells in the intracellular phase, which includes mucus production enhancement and the secretion of collagenases and collagenolytic enzymes in the intestines ( 18 , 20 ).…”

Section: Lessons From the Gut Health Impairment In Broilersmentioning

confidence: 99%

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Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production

Zhu

Sun²,

Zhang

et al. 2021

Front. Nutr.

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The perturbation of gut health is a common yet unresolved problem in broiler chicken production. Antibiotics used as growth promoters have remarkably improved the broiler production industry with high feed conversion efficiency and reduced intestinal problems. However, the misuse of antibiotics has also led to the increase in the development of antibiotic resistance and antibiotic residues in the meat. Many countries have enacted laws prohibiting the use of antibiotics in livestock production because of the increasing concerns from the consumers and the public. Consequently, one of the most significant discussions in the poultry industry is currently antibiotic-free livestock production. However, the biggest challenge in animal husbandry globally is the complete removal of antibiotics. The necessity to venture into antibiotic-free production has led researchers to look for alternatives to antibiotics in broiler chicken production. Many strategies can be used to replace the use of antibiotics in broiler farming. In recent years, many studies have been conducted to identify functional feed additives with similar beneficial effects as antibiotic growth promoters. Attention has been focused on prebiotics, probiotics, organic acids, emulsifiers, enzymes, essential oils, tributyrin, and medium-chain fatty acids. In this review, we focused on recent discoveries on gut health maintenance through the use of these functional feed additives as alternatives to antibiotics in the past 10 years to provide novel insights into the design of antibiotic-free feeds.

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Section: Lessons From the Gut Health Impairment In Broilersmentioning

confidence: 99%

Section: Lessons From the Gut Health Impairment In Broilersmentioning

confidence: 99%

Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production

Zhu

Sun²,

Zhang

et al. 2021

Front. Nutr.

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“…The delivery system of recombinant or subunit vaccine candidates, using eukaryote or prokaryote vectors for feed or water administration application have shown optimistic and realistic results [ 36 , 37 , 38 , 39 , 40 ]. Some of these novel vaccines not only provide antigenic diversity, but cost of production and welfare issues are significantly reduced or eliminated [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Juárez-Estrada

Gayosso-Vázquez

Téllez-Isaías

et al. 2021

Animals

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This study investigated protection against Eimeria tenella following the vaccination of chicks with 5.3 × 106 E. tenella whole-sporozoites emulsified in the nanoparticle adjuvant IMS 1313 N VG Montanide™ (EtSz-IMS1313). One-day-old specific pathogen-free (SPF) chicks were subcutaneously injected in the neck with EtSz-IMS1313 on the 1st and 10th days of age. Acquired immunity was assayed through a challenge with 3 × 104 homologous sporulated oocysts at 21 days of age. The anticoccidial index (ACI) calculated for every group showed the effectiveness of EtSz-IMS1313 as a vaccine with an ACI of 186; the mock-injected control showed an ACI of 18 and the unimmunized, challenged control showed an ACI of −28. In a comparison assay, antibodies from rabbits and SPF birds immunized with EtSz-IMS1313 recognized almost the same polypeptides in the blotting of E. tenella sporozoites and merozoites. However, rabbit antisera showed the clearest recognition pattern. Polypeptides of 120, 105, 94, 70, 38, and 19 kDa from both E. tenella life cycle stages were the most strongly recognized by both animal species. The E. tenella zoite-specific IgG antibodies from the rabbits demonstrated the feasibility for successful B cell antigen identification.

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“…Currently, numerous vaccines were reported on the pathogenic Eimeria species, such as E. tenella , E. acervalina , E. maxima , and E. necatrix ( 13 , 14 ). However, the vaccines against E. mitis did not cause wide public concern over the recent years, and the synergistic mechanisms after coinfection with E. mitis still remain unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Detailed research of the Eimeria life cycle has revealed many protein antigens that are recognized as potential candidates for vaccines ( 16 , 17 ). Examples include apical membrane antigen ( 14 ), microneme ( 17 , 18 ), surface antigen ( 19 ), immune mapped protein ( 16 ), and profilin ( 20 ). In addition, Liu et al ( 21 ) reported on the potential vaccine candidates of 14-3-3 proteins with a conserved sequence in parasites.…”

Section: Introductionmentioning

confidence: 99%

PLGA Nanospheres as Delivery Platforms for Eimeria mitis 1a Protein: A Novel Strategy to Improve Specific Immunity

et al. 2022

Front. Immunol.

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The infections of chicken coccidiosis impact the welfare of chickens and the economical production of poultry. Eimeria mitis is ubiquitous in chicken coccidiosis, and E. mitis infection can significantly affect the productivity of birds. Up to now, few efficient vaccines against E. mitis have been reported, whereas the recombinant subunit vaccines delivered by nanomaterials may elicit an encouraging outcome. Thus, in this study, we chose E. mitis 1a (Em1a) protein as the candidate antigen to generate Em1a preparations. The recombinant Em1a (rEm1a) protein was encapsulated with poly lactic-co-glycolic acid (PLGA) and chitosan (CS) nanospheres. The physical characterization of the rEm1a-PLGA and rEm1a-CS nanospheres was investigated, and the resulting nanospheres were proven to be nontoxic. The protective efficacy of rEm1a-PLGA and rEm1a-CS preparations was evaluated in E. mitis-challenged birds in comparison with two preparations containing rEm1a antigen emulsified in commercially available adjuvants. ELISA assay, flow cytometry analysis, and quantitative real-time PCR (qPCR) analysis indicated that vaccination with rEm1a-loaded nanospheres significantly upregulated the secretions of antibodies and cytokines and proportions of CD4+ and CD8+ T lymphocytes. Compared with the other three preparations, rEm1a-PLGA nanosphere was more effective in improving growth performance and inhibiting oocyst output in feces, indicating that the PLGA nanosphere was associated with optimal protection against E. mitis. Collectively, our results highlighted the advantages of nanovaccine in eliciting protective immunity and may provide a new perspective for developing effective vaccines against chicken coccidiosis.

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Transgenic Eimeria parasite: A potential control strategy for chicken coccidiosis

Cited by 21 publications

References 56 publications

Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production

Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

PLGA Nanospheres as Delivery Platforms for Eimeria mitis 1a Protein: A Novel Strategy to Improve Specific Immunity

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