2016
DOI: 10.1016/j.gene.2015.10.066
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Transgenic expression of green fluorescent protein in caprine embryos produced through electroporation-aided sperm-mediated gene transfer

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Cited by 14 publications
(6 citation statements)
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“…In addition to the suitability of the TCE method for transfecting cells, the associated transient electrical stimulation of cells also promotes the release of EVs carrying mRNA. 39 The negative electrode is in contact with the bottom of the solution containing DNA (GFP-expressing plasmid) and the cells are seeded on the top surface of the Transwell membrane. This method was found to result in the synthesis of GFP following transcription and translation from the plasmid, resulting in green fluorescence in the cells (Figure S6a).…”
Section: Tce System For Donor Cell Encapsulation Of Plns and Generati...mentioning
confidence: 99%
“…In addition to the suitability of the TCE method for transfecting cells, the associated transient electrical stimulation of cells also promotes the release of EVs carrying mRNA. 39 The negative electrode is in contact with the bottom of the solution containing DNA (GFP-expressing plasmid) and the cells are seeded on the top surface of the Transwell membrane. This method was found to result in the synthesis of GFP following transcription and translation from the plasmid, resulting in green fluorescence in the cells (Figure S6a).…”
Section: Tce System For Donor Cell Encapsulation Of Plns and Generati...mentioning
confidence: 99%
“…After a simple step of incubating sperm cells with exogenous DNA, transfected sperm cells can then be transferred to female (eggs) using various strategies, such as artificial insemination (AI) (Zhao et al, 2010), intracytoplasmic sperm injection (ICSI), in vitro fertilization (IVF), or laparoscopic insemination (LI) (Pereyra-Bonnet et al, 2011), with varied efficiencies. SMGT application in sheep and goats includes the production of both transgenic embryos (Pereyra-Bonnet et al, 2008; Shadanloo et al, 2010; Pereyra-Bonnet et al, 2011; Pramod et al, 2016) and transgenic founders (Zhao et al, 2010) using marker transgenes. Despite the simplicity this approach offers, its application for the generation of transgenic sheep and goats remained limited.…”
Section: Overview Of Genetic Modification and Relevant Biotechnologicmentioning
confidence: 99%
“…The cleavage rates of embryos obtained after IVF using semen from either experimental or control bucks were comparable in this study. In our earlier report, we observed 4.31% fluorescent embryos while using SMGT 43 . Previously, another group also produced transgenic embryos using intra-cytoplasmic sperm injection (ICSI) of fluorescent sperm in mice 15 .…”
Section: Discussionmentioning
confidence: 64%