Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana

Ding, Zhenhua; Li, Shiming; An, Xianghai; Liu, Xin; Qin, Huanju; Wang, Daowen

doi:10.1016/s1673-8527(09)60003-5

Cited by 315 publications

(201 citation statements)

References 54 publications

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“…Thus, it appears that TaCHP can act as a negative regulator of ABA biosynthesis, so that its down-regulation in response to salinity stress may partially account for the accumulation of ABA. Some MYB transcription factors are known to be required for ABA biosynthesis (Zhu et al, 2005), and the abundance of AtABA1 transcript was increased when AtMYB15 was overexpressed in nonstressed Arabidopsis seedlings (Ding et al, 2009). Here, the overexpression of TaCHP significantly reduced AtMYB15 transcription (Fig.…”

Section: Tachp Is Involved In An Aba-dependent Pathwaymentioning

confidence: 72%

TaCHP: A Wheat Zinc Finger Protein Gene Down-Regulated by Abscisic Acid and Salinity Stress Plays a Positive Role in Stress Tolerance

Zhao

et al. 2010

Plant Physiology

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The plant response to abiotic stresses involves both abscisic acid (ABA)-dependent and ABA-independent signaling pathways. Here we describe TaCHP, a CHP-rich (for cysteine, histidine, and proline rich) zinc finger protein family gene extracted from bread wheat (Triticum aestivum), is differentially expressed during abiotic stress between the salinity-sensitive cultivar Jinan 177 and its tolerant somatic hybrid introgression cultivar Shanrong No.3. TaCHP expressed in the roots of seedlings at the three-leaf stage, and the transcript localized within the cells of the root tip cortex and meristem. TaCHP transcript abundance was higher in Shanrong No.3 than in Jinan 177, but was reduced by the imposition of salinity or drought stress, as well as by the exogenous supply of ABA. When JN17, a salinity hypersensitive wheat cultivar, was engineered to overexpress TaCHP, its performance in the face of salinity stress was improved, and the ectopic expression of TaCHP in Arabidopsis (Arabidopsis thaliana) also improved the ability of salt tolerance. The expression level of a number of stress reporter genes (AtCBF3, AtDREB2A, AtABI2, and AtABI1) was raised in the transgenic lines in the presence of salinity stress, while that of AtMYB15, AtABA2, and AtAAO3 was reduced in its absence. The presence in the upstream region of the TaCHP open reading frame of the cis-elements ABRE, MYBRS, and MYCRS suggests that it is a component of the ABA-dependent and -independent signaling pathways involved in the plant response to abiotic stress. We suggest that TaCHP enhances stress tolerance via the promotion of CBF3 and DREB2A expression.

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Section: Tachp Is Involved In An Aba-dependent Pathwaymentioning

confidence: 72%

TaCHP: A Wheat Zinc Finger Protein Gene Down-Regulated by Abscisic Acid and Salinity Stress Plays a Positive Role in Stress Tolerance

Zhao

et al. 2010

Plant Physiology

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“…Myb tfs interact with ciselements in the promoter regions of several stress-related genes through ABA-dependent pathway of stress signalling for the upregulation of abiotic stress responsive genes. Overexpression of Myb tf results in improved drought and salt tolerance in Arabidopsis (Ding et al 2009), osmotic tolerance (Abe et al 2003), drought stress in tomato (Vannini et al 2007) and drought and cold tolerance in the apple transgenic plants (Pasquali et al 2008). Overexpression of a StMYB1R-1 transgene in potato plants improved tolerance to drought stress while not influencing significant effects on other agronomical traits (Shin et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

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In order to meet the ever rising global demand for natural rubber, cultivation of Hevea is being extended to nontraditional regions of India where extreme climatic conditions like drought and low temperature negatively influence the crop performance. In order to ensure maximum productivity, identification of drought tolerant clones of Hevea which can cope up with stress and give better crop yield is essential. Several attempts have been made previously to identify genes that are associated with drought tolerance in Hevea. In the present study, quantitative expression analysis was made using quantitative PCR for seven drought associated transcripts in four clones of Hevea with varying levels of drought tolerance. Among the seven genes studied, Mitogen Activated Protein (MAP) kinase, Myeloblastosis (Myb) transcription factor, C-repeat responsive element/Dehydration Responsive Element (CRT/DRE) binding factor and Nuclear Factor Y subunit A (NFYA) showed a positive association with drought tolerance. Transcripts of ascorbate peroxidase and heat shock protein 70 (HSP 70) did not show any correlation with drought tolerance. Interestingly, catalase gene was found down regulated in all the clones under drought condition. The possible role of these genes based on their level of gene expression in four different clones of Hevea with varying levels of drought tolerance is discussed.

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“…Generally, these TFs are thought to induce shikimate and phenylpropanoid metabolism in response to stress (Chen et al, 2006;Ding et al, 2009). In Lotus, increased activity of these proteins likely increases the flux through the GPP, providing substrates for the isoflavonoid pathway.…”

Section: Identification Of Tf Genes Specific To Lotus and Their Exprementioning

confidence: 99%

Transcription Factors of Lotus: Regulation of Isoflavonoid Biosynthesis Requires Coordinated Changes in Transcription Factor Activity

et al. 2012

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Isoflavonoids are a class of phenylpropanoids made by legumes, and consumption of dietary isoflavonoids confers benefits to human health. Our aim is to understand the regulation of isoflavonoid biosynthesis. Many studies have shown the importance of transcription factors in regulating the transcription of one or more genes encoding enzymes in phenylpropanoid metabolism. In this study, we coupled bioinformatics and coexpression analysis to identify candidate genes encoding transcription factors involved in regulating isoflavonoid biosynthesis in Lotus (Lotus japonicus). Genes encoding proteins belonging to 39 of the main transcription factor families were examined by microarray analysis of RNA from leaf tissue that had been elicited with glutathione. Phylogenetic analyses of each transcription factor family were used to identify subgroups of proteins that were specific to L. japonicus or closely related to known regulators of the phenylpropanoid pathway in other species. R2R3MYB subgroup 2 genes showed increased expression after treatment with glutathione. One member of this subgroup, LjMYB14, was constitutively overexpressed in L. japonicus and induced the expression of at least 12 genes that encoded enzymes in the general phenylpropanoid and isoflavonoid pathways. A distinct set of six R2R3MYB subgroup 2-like genes was identified. We suggest that these subgroup 2 sister group proteins and those belonging to the main subgroup 2 have roles in inducing isoflavonoid biosynthesis. The induction of isoflavonoid production in L. japonicus also involves the coordinated down-regulation of competing biosynthetic pathways by changing the expression of other transcription factors.

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Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana

Cited by 315 publications

References 54 publications

TaCHP: A Wheat Zinc Finger Protein Gene Down-Regulated by Abscisic Acid and Salinity Stress Plays a Positive Role in Stress Tolerance

TaCHP: A Wheat Zinc Finger Protein Gene Down-Regulated by Abscisic Acid and Salinity Stress Plays a Positive Role in Stress Tolerance

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Transcription Factors of Lotus: Regulation of Isoflavonoid Biosynthesis Requires Coordinated Changes in Transcription Factor Activity

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