Transgenic expression of the positive selected human GLUD2 gene improves in vivo glucose homeostasis by regulating basic insulin secretion

Petraki, Zoe; Droubogiannis, Stavros; Mylonaki, Konstantina; Chlouverakis, Gregory; Plaitakis, Andreas; Spanaki, Cleanthe

doi:10.1016/j.metabol.2019.153958

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…GLUD2, which was originally identified as a glutamate dehydrogenase (GDH)-coding gene, was deemed an evolutionarily novel version of GLUD1 [38] . Generally, GLUD2 mainly modulates glucose homeostasis by tuning fasting serum insulin levels and acts on the tricarboxylic acid cycle, potentially facilitating lipid biosynthesis [ 39 , 40 ]. However, a recent study revealed an ammonia-centered recycling mode mediated by tuning GDH to promote proliferation in breast cancer, which indicated multiple potential tumor metabolic-modulating roles of the GLUD2 protein [41] .…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level

Wei

Yang

et al. 2022

Translational Oncology

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Section: Discussionmentioning

confidence: 99%

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level

Wei

Yang

et al. 2022

Translational Oncology

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“…P26443 protein (gene Glud1) is mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate, which plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate and is an important intermediate in the TCA cycle ( Csibi et al, 2013 ). Also, it may be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate and can contribute to glucose-stimulated insulin secretion in murine β-cells, but not to basic insulin release ( Petraki et al, 2019 ; Roy et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis reveals the pathogenesis of db/db mice diabetic kidney disease and the treatment mechanisms of multi-bioactive compounds combination from Salvia miltiorrhiza

Xiang²,

Su³

et al. 2022

Front. Pharmacol.

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Diabetic kidney disease (DKD) is a common diabetic complication. Salvia miltiorrhiza has significant therapeutic effects on diabetes complications, although the mechanism remains unclear. Here, biochemical indicators and pathological changes were used to screen out the optimal Salvia miltiorrhiza multi-bioactive compounds combination. Metabolomics, transcriptomics and proteomics were used to explore the pathogenesis of DKD. RT-PCR and parallel reaction monitoring targeted quantitative proteome analysis were utilized to investigate treatment mechanisms of the optimal Salvia miltiorrhiza multi-bioactive compounds combination. The db/db mice showed biochemical abnormalities and renal lesions. The possible metabolic pathways were steroid hormone biosynthesis and sphingolipid metabolism. The 727 differential genes found in transcriptomics were associated with biochemical indicators via gene network to finally screen 11 differential genes, which were mainly key genes of TGF-β/Smad and PI3K/Akt/FoxO signaling pathways. Salvia miltiorrhiza multi-bioactive compounds combination could significantly regulate the Egr1, Pik3r3 and Col1a1 genes. 11 differentially expressed proteins involved in the two pathways were selected, of which 9 were significantly altered in db/db mice compared to db/m mice. Salvia miltiorrhiza multi-bioactive compounds combination could callback Q9DBM2, S4R1W1, Q91Y97, P47738, A8DUK4, and A2ARV4. In summary, Salvia miltiorrhiza multi-bioactive compounds combination may ameliorate kidney injury in diabetes through regulation of TGF-β/Smad and PI3K/Akt/FoxO signaling pathways.

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“…In the domain of GDH1, research by Vetterli et al, showed that GDH1 knockout leads to decreased obesity in diet-induced mice, suggesting a potential therapeutic target for obesity-related insulin resistance [ 66 ]. Lastly, research on GDH2 by Petraki and colleagues revealed that GDH2 knockin mice exhibit enhanced fasting serum insulin levels, pointing to its influence on fasting insulin regulation [ 67 ].…”

Section: Role Of Nadph-producing Enzymes In Diabetesmentioning

confidence: 99%

NADPH Dynamics: Linking Insulin Resistance and β-Cells Ferroptosis in Diabetes Mellitus

Moon

2023

IJMS

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This review offers an in-depth exploration of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) in metabolic health. It delves into how NADPH affects insulin secretion, influences insulin resistance, and plays a role in ferroptosis. NADPH, a critical cofactor in cellular antioxidant systems and lipid synthesis, plays a central role in maintaining metabolic homeostasis. In adipocytes and skeletal muscle, NADPH influences the pathophysiology of insulin resistance, a hallmark of metabolic disorders such as type 2 diabetes and obesity. The review explores the mechanisms by which NADPH contributes to or mitigates insulin resistance, including its role in lipid and reactive oxygen species (ROS) metabolism. Parallelly, the paper investigates the dual nature of NADPH in the context of pancreatic β-cell health, particularly in its relation to ferroptosis, an iron-dependent form of programmed cell death. While NADPH’s antioxidative properties are crucial for preventing oxidative damage in β-cells, its involvement in lipid metabolism can potentiate ferroptotic pathways under certain pathological conditions. This complex relationship underscores the delicate balance of NADPH homeostasis in pancreatic health and diabetes pathogenesis. By integrating findings from recent studies, this review aims to illuminate the nuanced roles of NADPH in different tissues and its potential as a therapeutic target. Understanding these dynamics offers vital insights into the development of more effective strategies for managing insulin resistance and preserving pancreatic β-cell function, thereby advancing the treatment of metabolic diseases.

show abstract

Transgenic expression of the positive selected human GLUD2 gene improves in vivo glucose homeostasis by regulating basic insulin secretion

Cited by 4 publications

References 28 publications

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level

Multi-omics analysis reveals the pathogenesis of db/db mice diabetic kidney disease and the treatment mechanisms of multi-bioactive compounds combination from Salvia miltiorrhiza

NADPH Dynamics: Linking Insulin Resistance and β-Cells Ferroptosis in Diabetes Mellitus

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