Transgenic Mouse Models Transferred into the Test Tube: New Perspectives for Developmental Toxicity Testing In Vitro ?

Kugler, Josephine; Luch, Andreas; Oelgeschläger, Michael

doi:10.1016/j.tips.2016.06.009

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…In future studies, more exploration of TCM and the active agents on the formed PF models is urgent need. Furthermore, alternative or combined models of PF should be recruited to confirm the pharmaceutical property of the active agents of TCM for supplement, either by chemical induction or using transgenic animals (Kugler et al, 2016;Moore and Hogaboam, 2008).…”

Section: Future Investigations Of Tcm In Pf Therapymentioning

confidence: 99%

Traditional Chinese medicine for pulmonary fibrosis therapy: Progress and future prospects

Kan

2017

Journal of Ethnopharmacology

115

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These research work have shown the therapeutic benefits of TCM in the treatment of PF. However, more continued researches should be undertaken to clarify the unconfirmed chemical composition and regulatory mechanisms, conduct standard clinical trials, and evaluate the possible side effects. The insights provided in present review will be needed for further exploration of botanical drugs in the development of PF therapy.

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Section: Future Investigations Of Tcm In Pf Therapymentioning

confidence: 99%

Traditional Chinese medicine for pulmonary fibrosis therapy: Progress and future prospects

Kan

2017

Journal of Ethnopharmacology

115

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A novel dual reporter embryonic stem cell line for toxicological assessment of teratogen-induced perturbation of anterior–posterior patterning of the heart

2019

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Reliable in vitro models to assess developmental toxicity of drugs and chemicals would lead to improvement in fetal safety and a reduced cost of drug development. The validated embryonic stem cell test (EST) uses cardiac differentiation of mouse embryonic stem cells (mESCs) to predict in vivo developmental toxicity, but does not take into account the stage-specific patterning of progenitor populations into anterior (ventricular) and posterior (atrial) compartments. In this study, we generated a novel dual reporter mESC line with fluorescent reporters under the control of anterior and posterior cardiac promoters. Reporter expression was observed in nascent compartments in transgenic mouse embryos, and mESCs were used to develop differentiation assays in which chemical modulators of Wnt (XAV939: 3, 10 µM), retinoic acid (all-trans retinoic acid: 0.1, 1, 10 µM; 9-cis retinoic acid: 0.1, 1, 10 µM; bexarotene 0.1, 1, 10 µM), and Tgf-β (SB431542: 3, 10 µM) pathways were tested for stage-and dose-dependent effects on in vitro anterior-posterior patterning. Our results suggest that with further development, the inclusion of anterior-posterior reporter expression could be part of a battery of high-throughput tests used to identify and characterize teratogens.

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Fundamental Concepts, Current Regulatory Design and Interpretation

Makris¹

2018

Comprehensive Toxicology

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Transgenic Mouse Models Transferred into the Test Tube: New Perspectives for Developmental Toxicity Testing In Vitro ?

Cited by 3 publications

References 69 publications

Traditional Chinese medicine for pulmonary fibrosis therapy: Progress and future prospects

Traditional Chinese medicine for pulmonary fibrosis therapy: Progress and future prospects

A novel dual reporter embryonic stem cell line for toxicological assessment of teratogen-induced perturbation of anterior–posterior patterning of the heart

Fundamental Concepts, Current Regulatory Design and Interpretation

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