Transgenic plants as genetic models for studying functions of plant genes

Influence of the high infectious load of the causal agent of bacterial ring rot of potatoes on variations in hydrogen peroxide and cAMP levels in transgenic potato plants <i>in vitro</i> with increased contents of endogenous hydrogen peroxide

“…В целях повышения защитных свойств и создания устойчивых форм растений эффективным подходом, в дополнение к традиционным методам селекции, является генетическая инженерия. На сегодняшний день трансгенные расте-ния широко используются в качестве модельных систем для решения различных задач [1,2].…”

Increased ribonuclease activity in Solanum tuberosum L. transformed with heterologous genes of apoplastic ribonucleases as a putative approach for production of virus resistant plants

“…This extracellular RNase is transported from cell to apoplast. Hypothetical mechanism of the antiviral effect of extracellular RNase can be based both on the vulnerability of the genomic DNA of the viruses at some stages of their penetration into the plant cell and on the simulation of the programmed cell death: when the integrity of tissues is disturbed, the content of apoplast can penetrate the cytoplasm of damaged cells, in which case active RNases function as "killer" proteins that kill the cell and prevent replication of the genomic RNA of the virus that penetrated it (Kochetov and Shumny, 2017). Destruction of viral RNA by RNases retards the development of the symptoms, mitigate their severity or localize the infection (Trifonova et al, 2007).…”

“…Destruction of viral RNA by RNases retards the development of the symptoms, mitigate their severity or localize the infection (Trifonova et al, 2007). The number of investigations devoted to transgenic plants with heterologous extracellular RNases is rather limited (Kochetov and Shumny, 2017). This approach provides an opportunity to obtain plants resistant to the broad spectrum of viruses (Sangaev et al, 2007;Trifonova et al, 2012).…”

Auxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco

“…The genetic transformation of model plants is key to validating gene function. Transgenic plants can be used to characterize single genes and reconstruct gene networks that control plant biochemistry, physiology, and morphology under different conditions (Kochetov and Shumny 2017). Molecular characterization is necessary after transformation because copy number and the insertion site can influence gene stability and the expression levels of the SS Lopes et al…”