Transgenic plants expressing immunosuppressive dsRNA improve entomopathogen efficacy against Spodoptera littoralis larvae

Lelio, Ilaria Di; Barra, Enrica; Coppola, Mariangela; Corrado, Giandomenico; Rao, Rosa; Caccia, Silvia

doi:10.1007/s10340-021-01467-z

Cited by 10 publications

(7 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aizawaii, Bta) is enhanced when target insects are immune impaired by RNAi-mediated silencing of genes involved in cellular immune responses 46,47 . In addition, this approach can be exploited in the field by co-administration of the Bta-based formulate with transformed bacteria or plants as delivery vectors for immune silencing dsRNAs 48,49 . Similarly, MosChito rafts could be used as vectors for Bti in association with dsRNA nanocarriers [50][51][52] or dsRNA-expressing microorganisms, as mosquito larvae have proved to be susceptible to environmental RNAi vectored by microorganisms, including S. cerevisiae 34,[53][54][55][56] .…”

Section: Discussionmentioning

confidence: 99%

MosChito rafts as effective and eco-friendly tool for the delivery of a Bacillus thuringiensis-based insecticide to Aedes albopictus larvae

Pitton

Negri

Pezzali

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Adult mosquito females, through their bites, are responsible for the transmission of different zoonotic pathogens. Although adult control represents a pillar for the prevention of disease spread, larval control is also crucial. Herein we characterized the effectiveness of a suitable tool, named “MosChito raft”, for the aquatic delivery of a Bacillus thuringiensis var. israelensis (Bti) formulate, a bioinsecticide active by ingestion against mosquito larvae. MosChito raft is a floating tool composed by chitosan cross-linked with genipin in which a Bti-based formulate and an attractant have been included. MosChito rafts (i) resulted attractive for the larvae of the Asian tiger mosquito Aedes albopictus, (ii) induced larval mortality within a few hours of exposure and, more importantly, (iii) protected the Bti-based formulate, whose insecticidal activity was maintained for more than one month in comparison to the few days residual activity of the commercial product. The delivery method was effective in both laboratory and semi-field conditions, demonstrating that MosChito rafts may represent an original, eco-based and user-friendly solution for larval control in domestic and peri-domestic aquatic habitats such as saucers and artificial containers in residential or urban environments.

show abstract

Section: Discussionmentioning

confidence: 99%

MosChito rafts as effective and eco-friendly tool for the delivery of a Bacillus thuringiensis-based insecticide to Aedes albopictus larvae

Pitton

Negri

Pezzali

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The silencing of Sl102 by RNAi caused an impairment in the nodulation and encapsulation responses of the hemocytes [ 16 , 44 , 135 ]. This was associated with a significantly increased susceptibility of the host larvae to the Cry toxin and Bt [ 16 , 44 , 135 , 136 ].…”

Section: Immunity Induced By Btmentioning

confidence: 99%

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Xiao

Yao

Bai

et al. 2023

Insects

View full text Add to dashboard Cite

Bacillus thuringiensis (Bt) is the safest, economically successful entomopathogen to date. It is extensively produced in transgenic crops or used in spray formulations to control Lepidopteran pests. The most serious threat to the sustainable usage of Bt is insect resistance. The resistance mechanisms to Bt toxins depend not only on alterations in insect receptors, but also on the enhancement of insect immune responses. In this work, we review the current knowledge of the immune response and resistance of insects to Bt formulations and Bt proteins, mainly in Lepidopteran pests. We discuss the pattern recognition proteins for recognizing Bt, antimicrobial peptides (AMPs) and their synthetic signaling pathways, the prophenoloxidase system, reactive oxygen species (ROS) generation, nodulation, encapsulation, phagocytosis, and cell-free aggregates, which are involved in immune response reactions or resistance to Bt. This review also analyzes immune priming, which contributes to the evolution of insect resistance to Bt, and puts forward strategies to improve the insecticidal activity of Bt formulations and manage insect resistance, targeting the insect immune responses and resistance.

show abstract

“…The Colorado potato beetle ( Zhang et al, 2015 ), tobacco whitefly and tobacco hornworm, Manduca sexta ( Burke et al, 2019 ), were all killed by chloroplast-expressed dsRNAs ( Dong et al, 2020 ). Di Lelio et al (2022) reported that when Spodoptera littoralis larvae eat tobacco plants expressing a dsRNA targeting the Sl 102 immune gene, the gene get silenced (et al). RNAi technology is effective in knocking down target genes in a variety of insect orders, including Diabrotica v. virgifera , maize rootworm larvae ( Khajuria et al, 2015 ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

View full text Add to dashboard Cite

Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

show abstract

Transgenic plants expressing immunosuppressive dsRNA improve entomopathogen efficacy against Spodoptera littoralis larvae

Cited by 10 publications

References 90 publications

MosChito rafts as effective and eco-friendly tool for the delivery of a Bacillus thuringiensis-based insecticide to Aedes albopictus larvae

MosChito rafts as effective and eco-friendly tool for the delivery of a Bacillus thuringiensis-based insecticide to Aedes albopictus larvae

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Biotechnological Approaches for Host Plant Resistance to Insect Pests

Contact Info

Product

Resources

About