1994
DOI: 10.1007/bf00233291
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Transgenic plums (Prunus domestica L.) express the plum pox virus coat protein gene

Abstract: Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidase genes. Integration and expression of the transferred genes into regenerated plum plants was verified through kan resistance, GUS assays, and PCR amplification of the PPV-CP gene. Twenty-two tran… Show more

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Cited by 167 publications
(124 citation statements)
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“…Genetic transformation in European plum (Prunus domestica L.) has been established through regeneration of the cotyledon and hypocotyl sections of mature embryos (Mante et al, 1989;Scorza et al, 1994), immature embryos and embryonic axes (Tian et al, 2007a;Srinivasan et al, 2012;Petri et al, 2012: Wang et al, 2013. Medium supplemented with thidiazuron (TDZ) 7.5 μM in combination with indole-3-butyric acid (IBA) 0.25 μM has been used for shoot regeneration, with a transformation effi ciency of 1.2% (Scorza et al, 1994;González-Padilla et al, 2003).…”
Section: Genetic Transformationmentioning
confidence: 99%
See 1 more Smart Citation
“…Genetic transformation in European plum (Prunus domestica L.) has been established through regeneration of the cotyledon and hypocotyl sections of mature embryos (Mante et al, 1989;Scorza et al, 1994), immature embryos and embryonic axes (Tian et al, 2007a;Srinivasan et al, 2012;Petri et al, 2012: Wang et al, 2013. Medium supplemented with thidiazuron (TDZ) 7.5 μM in combination with indole-3-butyric acid (IBA) 0.25 μM has been used for shoot regeneration, with a transformation effi ciency of 1.2% (Scorza et al, 1994;González-Padilla et al, 2003).…”
Section: Genetic Transformationmentioning
confidence: 99%
“…Medium supplemented with thidiazuron (TDZ) 7.5 μM in combination with indole-3-butyric acid (IBA) 0.25 μM has been used for shoot regeneration, with a transformation effi ciency of 1.2% (Scorza et al, 1994;González-Padilla et al, 2003). Petri et al (2012) reported 42% transformation effi ciency increase using 9 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for three days during co-cultivation, followed by a regeneration medium containing TDZ (7.5 μM) and kanamycin 80 mg L-1 as selective agent.…”
Section: Genetic Transformationmentioning
confidence: 99%
“…While the use of resistant cultivars represents the most effective solution to control sharka, a century of breeding has produced few highly resistant cultivars and the genetic basis of resistance remains elusive. As an enhancement to classical breeding, genetic engineering was used to produce transgenic clones that contain the PPV coat protein (CP) gene, applying the principle of pathogen-derived resisntance [5]. A result of this effort was the development of a transgenic clone designated as C5 (cv.…”
Section: Introductionmentioning
confidence: 99%
“…In the context of the paucity of natural resistance, few transgenic clones of Prunus domestica L. carrying the CP gene of Plum pox virus (PPV) were developed to obtain PPV resistant plums (Scorza et al, 1994). One transgenic line, C5, subsequently named 'HoneySweet' (Scorza et al, 2007) …”
Section: Introductionmentioning
confidence: 99%