Transgenic poplar expressing the pine GS1a show alterations in nitrogen homeostasis during drought

Molina-Rueda, Juan Jesús; Kirby, Edward G.

doi:10.1016/j.plaphy.2015.06.009

Cited by 25 publications

(14 citation statements)

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“…Furthermore, several studies reported that droughtstress related genes are significantly regulated at the transcription level by N fertilization or starvation (Euring et al 2014, suggesting that the N metabolism is linked with stress tolerance. This is supported by the finding that poplars overexpressing glutamine synthetase showed higher N uptake rates and higher drought tolerance than wild-type plants (Molina-Rueda & Kirby 2015). Therefore, the higher N uptake and N concentrations in leaf and stem tissues of the clone Max1 found in this study (Tab.…”

Section: Decreases In Wood Nitrogen Use Efficiency As An Adaptation Tsupporting

confidence: 84%

“…N storage and remobilization are particularly important to meet the N demand of forest trees for continuous growth (Rennenberg et al 2010). Poplar species differ in the way they take up and assimilate N (Luo et al 2013, Gan et al 2015, which may impact their competitive ability for soil nutrients and resistance to environmental stresses (Castro-Rodríguez et al 2015, Molina-Rueda & Kirby 2015. Several studies have focused on identifying and quantifying the best management practices to successfully establish hybrid poplar plantations under different nitrogen regimes (Lee & Jose 2003, Euring et al 2012.…”

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confidence: 99%

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Growth performance and nitrogen use efficiency of two Populus hybrid clones (P. nigra × P. maximowiczii and P. trichocarpa × P. maximowiczii) in relation to soil depth in a young plantation

Euring¹,

Ayegbeni²,

Jansen³

et al. 2016

iForest

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Growth performance and nitrogen use efficiency of two Populus hybrid clones (P. nigra × P. maximowiczii and P. trichocarpa × P. maximowiczii) in relation to soil depth in a young plantation Dejuan Euring It is a challenge to produce woody crops on marginal land. The goal of this study was to examine growth responses and nitrogen use efficiency of different poplar species on shallow soil. Typical biomass poplar clones of Max1 (P. nigra × P. maximowiczii) and H275 (P. trichocarpa × P. maximowiczii) were planted on a marginal site where a gradient in soil depth was present. The growth, biomass production, and nitrogen uptake rate as well as nitrogen use efficiency of Max1 and H275 were determined for three consecutive years. Both poplar clones showed decreased growth and biomass production in the shallow soil. Max1 showed better adaptation to shallow soil with higher survival rate and more biomass production than H275. Max1 had lower nitrogen use efficiency on shallow soil than H275. The results suggest that higher nitrogen uptake of poplar species might be an important adaptation to maintain productivity under unfavorable soil conditions. Keywords: Biomass, Nitrogen Use Efficiency, Poplar, Shallow Soil IntroductionWoody biomass is considered as a sustainable alternative source of energy for fossil fuels (Pleguezuelo et al. 2015). Short rotation woody crops such as poplar trees (Populus spp.) are well suited for woody biomass production because they are highly productive and can be managed using agronomic techniques (Kauter et al. 2003). To avoid competition for fertile land with food production, poplar plantations are expected to be established on marginal land which is less suitable for agriculture because of limited water and nutrient availability (Shortall 2013). Since the productivity of a poplar plantation depends on the proper selection of genotypes (Zalesny et al. 2011), research is needed to identify poplar cultivars with high survival rates, high biomass productivity and high disease resistance for cultivation on marginal soil ).Shallow soil with limited rooting depth, low drainage and high stone content are typical features of marginal land (Jiang et al. 2014). Rooting depth is an important determinant for the successful establishment and production of woody biomass (Burgess et al. 2015). N storage and cycling are vital processes for growth, adaptation and productivity of poplar trees (Millard & Grelet 2010). N storage and remobilization are particularly important to meet the N demand of forest trees for continuous growth (Rennenberg et al. 2010). Poplar species differ in the way they take up and assimilate N (Luo et al. 2013, Gan et al. 2015, which may impact their competitive ability for soil nutrients and resistance to environmental stresses (Castro-Rodríguez et al. 2015, Molina-Rueda & Kirby 2015. Several studies have focused on identifying and quantifying the best management practices to successfully establish hybrid poplar plantations under different nitrogen regimes (Lee & Jose 2003, Euring et al....

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Section: Decreases In Wood Nitrogen Use Efficiency As An Adaptation Tsupporting

confidence: 84%

mentioning

confidence: 99%

Growth performance and nitrogen use efficiency of two Populus hybrid clones (P. nigra × P. maximowiczii and P. trichocarpa × P. maximowiczii) in relation to soil depth in a young plantation

Euring¹,

Ayegbeni²,

Jansen³

et al. 2016

iForest

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“…As the first enzyme in the N assimilatory pathway, GS1s have been proven to play a critical partition role in plant N assimilation and metabolism, growth, and productivity ( Fuentes et al, 2001 ; Habash et al, 2001 ; Oliveira et al, 2002 ; Man et al, 2005 ; Molina-Rueda and Kirby, 2015 ; Seger et al, 2015 ; Guan et al, 2016 ). In this study, a cytoplasmic GS1 type protein PsnGS1.2 , was isolated from P. simonii × P. nigra and characterized by overexpression in tobacco.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Poplar PsnGS1.2 Overexpression on Growth, Secondary Cell Wall, and Fiber Characteristics in Tobacco

Liu

Wei

et al. 2018

Front. Plant Sci.

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The glutamine synthetase (GS1) is a key enzyme that catalyzes the reaction of glutamate and ammonia to produce glutamine in the nitrogen (N) metabolism. Previous studies on GS1s in several plant species suggest that overexpression of GS1s can enhance N utilization, accelerate plant vegetative growth, and change wood formation. In this study, we isolated a GS1 gene, termed PsnGS1.2, from Populus simonii × Populus nigra. This gene was expressed at a higher level in roots, and relatively lower but detectable levels in xylem, leaves and phloem of P. simonii × P. nigra. The protein encoded by PsnGS1.2 is primarily located in the cytoplasm. Overexpression of PsnGS1.2 in tobacco led to the increased GS1 activity and IAA content, the augmented N assimilation, and the enlarged leaves with altered anatomical structures. These changes presumably promoted photosynthetic, growth, and biomass productivity. It was noteworthy that the secondary cell walls and fiber characteristics changed remarkably in PsnGS1.2 transgenic tobacco. These changes aligned well with the altered expression levels of the genes involved in fiber development, secondary cell wall component biosynthesis, IAA biosynthesis, amino acid transport, and starch breakdown. Taken together, the results from our study suggest that catalytic functions of PsnGS1.2 on N assimilation and metabolism in transgenic tobacco had significant effects on vegetative growth, leaf development, and secondary cell wall formation and properties through acceleration of photosynthesis and IAA biosynthesis, and redirection of carbon flux to synthesis of more cellulose and hemicellulose.

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“…Furthermore, the observed phenotype as a consequence of transgene expression was related to the correct assembly of GS1 subunits in the cytosol of photosynthetic cells. Further characterization showed that GS transgenics had a better NUE ( Man et al, 2005 ; Castro-Rodríguez et al, 2017 ) and enhanced tolerance to abiotic stress ( El-Khatib et al, 2004 ; Pascual et al, 2008 ; Shestibratov et al, 2010 ; Molina-Rueda and Kirby, 2015 ). A similar approach, overexpressing GS1 , has also been used to improve NUE in birch species ( Lebedev et al, 2017 ).…”

Section: Gene Functional Analysis In Transgenic Treesmentioning

confidence: 99%

Nitrogen Metabolism and Biomass Production in Forest Trees

et al. 2018

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Low nitrogen (N) availability is a major limiting factor for tree growth and development. N uptake, assimilation, storage and remobilization are key processes in the economy of this essential nutrient, and its efficient metabolic use largely determines vascular development, tree productivity and biomass production. Recently, advances have been made that improve our knowledge about the molecular regulation of acquisition, assimilation and internal recycling of N in forest trees. In poplar, a model tree widely used for molecular and functional studies, the biosynthesis of glutamine plays a central role in N metabolism, influencing multiple pathways both in primary and secondary metabolism. Moreover, the molecular regulation of glutamine biosynthesis is particularly relevant for accumulation of N reserves during dormancy and in N remobilization that takes place at the onset of the next growing season. The characterization of transgenic poplars overexpressing structural and regulatory genes involved in glutamine biosynthesis has provided insights into how glutamine metabolism may influence the N economy and biomass production in forest trees. Here, a general overview of this research topic is outlined, recent progress are analyzed and challenges for future research are discussed.

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Transgenic poplar expressing the pine GS1a show alterations in nitrogen homeostasis during drought

Cited by 25 publications

References 76 publications

Growth performance and nitrogen use efficiency of two Populus hybrid clones (P. nigra × P. maximowiczii and P. trichocarpa × P. maximowiczii) in relation to soil depth in a young plantation

Growth performance and nitrogen use efficiency of two Populus hybrid clones (P. nigra × P. maximowiczii and P. trichocarpa × P. maximowiczii) in relation to soil depth in a young plantation

The Effect of Poplar PsnGS1.2 Overexpression on Growth, Secondary Cell Wall, and Fiber Characteristics in Tobacco

Nitrogen Metabolism and Biomass Production in Forest Trees

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