Transgenic quail to dynamically image amniote embryogenesis

Huss, David; Bénazéraf, Bertrand; Wallingford, Allison; Filla, Michael B.; Yang, Jennifer; Fraser, Scott E.; Lansford, Rusty

doi:10.1242/dev.121392

Cited by 48 publications

(52 citation statements)

References 50 publications

(69 reference statements)

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“…2). We propose that the dynamic protrusive activity of the filopodia might help to rearrange the pre-existing FN fibrils into pillars, possibly finetuning interactions between tissues that are elongating and expanding at different rates during embryo growth (Huss et al, 2015). The heart beat-driven pulsations of the dorsal aortae also appear to help establish, pattern and/or stabilize the FN pillars.…”

Section: Spatiomechanical Organization Of Fn Pillars In the Somiteendmentioning

confidence: 97%

“…To dynamically study how filopodia determine nuclear localization in the somitic epithelium, we performed time-lapse imaging analysis of H2B-EGFP-2A-FP4-mito-electroporated somitic cells in PGK:H2B-mCherry transgenic quail embryos (Huss et al, 2015). In somite cells expressing control plasmid (H2B-EGFP-2A-AP4-mito), H2B-EGFP + /H2B-mCherry + nuclei localized closer to the basal side of the somite epithelium, as was seen for nonelectroporated cells (mCherry + , n=5, Fig.…”

Section: Basal Filopodia Are Required For Somitic Cell Polarizationmentioning

confidence: 99%

“…The PGK:H2B-mCherry transgenic quail (Huss et al, 2015) was obtained from the Department of Animal Resource, University of Southern California (USA). Quail strains were bred at Animal Resources and Development, Kumamoto University (Japan) and the quail breeding facility at Kyushu University (Japan) according to Huss et al (2008).…”

Section: Quail Embryosmentioning

confidence: 99%

“…We discovered these FN pillars by studying FN distribution in a transgenic quail embryo model system, using tissue-specific DNA electroporation and time-lapse imaging (Bower et al, 2011). The PGK:H2B-mCherry transgenic quail embryo enables us to track every cell and ECM dynamics over time in living embryos, and to study cellautonomous roles of electroporated genes (Huss et al, 2015). This approach allowed us to visualize the previously unknown pattern of FN in the somite-endoderm gap.…”

Section: Introductionmentioning

confidence: 99%

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Basal filopodia and vascular mechanical stress organize fibronectin into pillars bridging the mesoderm-endoderm gap

et al. 2017

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Cells may exchange information with other cells and tissues by exerting forces on the extracellular matrix (ECM). Fibronectin (FN) is an important ECM component that forms fibrils through cell contacts and creates directionally biased geometry. Here, we demonstrate that FN is deposited as pillars between widely separated germ layers, namely the somitic mesoderm and the endoderm, in quail embryos. Alongside the FN pillars, long filopodia protrude from the basal surfaces of somite epithelial cells. Loss-of-function of Ena/VASP, α5β1-integrins or talin in the somitic cells abolished the FN pillars, indicating that FN pillar formation is dependent on the basal filopodia through these molecules. The basal filopodia and FN pillars are also necessary for proper somite morphogenesis. We identified a new mechanism contributing to FN pillar formation by focusing on cyclic expansion of adjacent dorsal aorta. Maintenance of the directional alignment of the FN pillars depends on pulsatile blood flow through the dorsal aortae. These results suggest that the FN pillars are specifically established through filopodia-mediated and pulsating force-related mechanisms.

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Section: Spatiomechanical Organization Of Fn Pillars In the Somiteendmentioning

confidence: 97%

Section: Basal Filopodia Are Required For Somitic Cell Polarizationmentioning

confidence: 99%

Section: Quail Embryosmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Basal filopodia and vascular mechanical stress organize fibronectin into pillars bridging the mesoderm-endoderm gap

et al. 2017

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“…Specific flocks, such as the Roslin Green eGFP line (also known as Glo-chicks (Fig. 5A), available in the US through Susan Chapman at Clemson University), H2B-YFP, Tie1-GFP and PGK-mCherry (available through the Ozark egg company) or Tg(PGK1:H2B-chFP) quails (Huss et al, 2015) can be widely used by developmental biologists, and make it possible to generate chimeras between fluorescent and non-fluorescent embryos (McGrew et al, 2008) (Fig. 5A).…”

Section: Introductionmentioning

confidence: 99%

Utilizing the chicken as an animal model for human craniofacial ciliopathies

Schock

Chang

Youngworth

et al. 2016

Developmental Biology

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The chicken has been a particularly useful model for the study of craniofacial development and disease for over a century due to their relatively large size, accessibility, and amenability for classical bead implantation and transplant experiments. Several naturally occurring mutant lines with craniofacial anomalies also exist and have been heavily utilized by developmental biologist for several decades. Two of the most well known lines, talpid2 (ta2) and talpid3 (ta3), represent the first spontaneous mutants to have the causative genes identified. Despite having distinct genetic causes, both mutants have recently been identified as ciliopathic. Excitingly, both of these mutants have been classified as models for human craniofacial ciliopathies: Oral-facial-digital syndrome (ta2) and Joubert syndrome (ta3). Herein, we review and compare these two models of craniofacial disease and highlight what they have revealed about the molecular and cellular etiology of ciliopathies. Furthermore, we outline how applying classical avian experiments and new technological advances (transgenics and genome editing) with naturally occurring avian mutants can add a tremendous amount to what we currently know about craniofacial ciliopathies.

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Diverse contribution of amniogenic somatopleural cells to cardiovascular development: With special reference to thyroid vasculature

Haneda

Miyagawa‐Tomita

Uchijima

et al. 2022

Developmental Dynamics

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Background:The somatopleure serves as the primordium of the amnion, an extraembryonic membrane surrounding the embryo. Recently, we have reported that amniogenic somatopleural cells (ASCs) not only form the amnion but also migrate into the embryo and differentiate into cardiomyocytes and vascular endothelial cells. However, detailed differentiation processes and final distributions of these intra-embryonic amniogenic somatopleural cells (hereafter referred to as iASCs) remain largely unknown.Results: By quail-chick chimera analysis, we here show that iASCs differentiate into various cell types including cardiomyocytes, smooth muscle cells, cardiac interstitial cells, and vascular endothelial cells. In the pharyngeal region, they distribute selectively into the thyroid gland and differentiate into vascular endothelial cells to form intra-thyroid vasculature.Explant culture experiments indicated sequential requirement of FGF and VEGF signaling for endothelial differentiation of iASCs. Single-cell transcriptome analysis further revealed heterogeneity and the presence of hemangioblast-like cell population within ASCs, with a switch from FGF to VEGF receptor gene expression. Conclusion:The present study demonstrates novel roles of amniogenic somatopleural cells especially in heart and thyroid development. It will provide a novel clue for understanding the cardiovascular development of amniotes from embryological and evolutionary perspectives.

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Transgenic quail to dynamically image amniote embryogenesis

Cited by 48 publications

References 50 publications

Basal filopodia and vascular mechanical stress organize fibronectin into pillars bridging the mesoderm-endoderm gap

Basal filopodia and vascular mechanical stress organize fibronectin into pillars bridging the mesoderm-endoderm gap

Utilizing the chicken as an animal model for human craniofacial ciliopathies

Diverse contribution of amniogenic somatopleural cells to cardiovascular development: With special reference to thyroid vasculature

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