2021
DOI: 10.1038/s41598-021-03229-4
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Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus

Abstract: The areas where dengue virus (DENV) is endemic have expanded rapidly, driven in part by the global spread of Aedes species, which act as disease vectors. DENV replicates in the mosquito midgut and is disseminated to the mosquito’s salivary glands for amplification. Thus, blocking virus infection or replication in the tissues of the mosquito may be a viable strategy for reducing the incidence of DENV transmission to humans. Here we used the mariner Mos1 transposase to create an Aedes aegypti line that expresses… Show more

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Cited by 9 publications
(8 citation statements)
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“…One approach termed population modification, or population replacement, aims to modify the target population with desired traits. In the mosquito field, for example, many groups have developed mosquitoes that are incapable of transmitting one or more deadly human pathogens (6)(7)(8)(9)(10)(11)(12)(13). If these diseaseresistant mosquitoes are robust enough to stably function in diverse natural pathogen populations (14) and then released into the environment such that they achieve a high enough frequency in the population, then the population could have reduced or eliminated disease transmission capacity [reviewed in (14)(15)(16)].…”
Section: Overview Of Genetic Control Strategiesmentioning
confidence: 99%
“…One approach termed population modification, or population replacement, aims to modify the target population with desired traits. In the mosquito field, for example, many groups have developed mosquitoes that are incapable of transmitting one or more deadly human pathogens (6)(7)(8)(9)(10)(11)(12)(13). If these diseaseresistant mosquitoes are robust enough to stably function in diverse natural pathogen populations (14) and then released into the environment such that they achieve a high enough frequency in the population, then the population could have reduced or eliminated disease transmission capacity [reviewed in (14)(15)(16)].…”
Section: Overview Of Genetic Control Strategiesmentioning
confidence: 99%
“…aegypti genome and various transcriptomes [8][9][10], pivotal tools in the development of genetic-based vector control strategies have been the reduction of transcript levels using RNAi [11], germline transformation using transposable elements [12], and programmable DNA targeting-using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) [13][14][15]. For example, RNAi and other small RNAs have been instrumental in generating knock-down phenotypes within the mosquito [16][17][18] as well as a method for reducing viral transcript numbers [18][19][20][21][22]. Moreover, CRISPR/Cas9 has been pivotal for site-directed mutagenesis and site-specific recombination to become more efficient, precise, and accessible [14,23].…”
Section: Introductionmentioning
confidence: 99%
“…Currently there are no approved vaccines available to prevent infection of humans with any pathogenic alphaviruses and the only preventative method is vector control. Control via genetic modification of the mosquito vector to achieve a virus-refractory effect, is a novel approach to overcome this problem (23)(24)(25). Of particular interest would be a broad-spectrum effect (i.e.…”
Section: Introductionmentioning
confidence: 99%