Transgenic Resistance

Cillo, Fabrizio; Palukaitis, Peter

doi:10.1016/b978-0-12-801246-8.00002-0

Cited by 57 publications

(27 citation statements)

References 576 publications

(375 reference statements)

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“…Recently, Yang et al showed the development of marker-free resistance by segregation for geminivirus [50]. The antibiotic-free resistance line 630 was highly resistant to TYLCV, and the virus could not be detected in resistant plants for more than two months, although about 5 % of seedlings show resistance breakage, as has been noted in many studies with RNA and DNA viruses [6,41,51]. We hypothesize that virus genome variations (mutagenesis) might be associated with the resistance-breaking phenomenon; however, no sequence changes were found in the transgene region within the resistance-breaking plants.…”

Section: Size and Distribution Of T-sirna In Transgenic Rootstock Andmentioning

confidence: 84%

“…These results demonstrate that even though t-siRNA could move into the scion via a graft union, the scions were not resistant to TYLCV infection. [38] and successfully subsequently demonstrated for many RNA and DNA viruses infecting different crops [6]. The main restrictions for PTGS-based resistance are the sequence specificity and the variable ratio of resistance of the progeny.…”

Section: Size and Distribution Of T-sirna In Transgenic Rootstock Andmentioning

confidence: 99%

“…The presence of small interfering RNA (siRNA) is a hallmark of the activated RNA silencing process, and several studies have demonstrated a correlation between viraltransgene-siRNA accumulation and virus resistance [6]. In this study, a silencing construct (pBIN35S:IR-TYLCV) was designed to produce transgene-dsRNA that would be effectively recognized and diced into siRNAs by the plant RNA silencing machinery.…”

Section: Accumulation Of T-sirna In Transgenic Tomato Linesmentioning

confidence: 99%

“…Breeding programs by introgression of resistance or tolerance genes from wild species have been successful [5]. Genetic engineering for virus resistance has an advantage in that through this process it is possible to achieve a high level of virus resistance rapidly [6]. Several approaches to transgenic pathogen-derived resistance were examined in the case of TYLCV: a. coat-protein-mediated Diana Leibman, Shanmugam Prakash and Dalia Wolf have contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

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Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

et al. 2015

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Gene silencing is a natural defense response of plants against invading RNA and DNA viruses. The RNA post-transcriptional silencing system has been commonly utilized to generate transgenic crop plants that are "immune" to plant virus infection. Here, we applied this approach against the devastating DNA virus tomato yellow leaf curl virus (TYLCV) in its host tomato (Solanum lycopersicum L.). To generate broad resistance to a number of different TYLCV viruses, three conserved sequences (the intergenic region [NCR], V1-V2 and C1-C2 genes) from the genome of the severe virus (TYLCV) were synthesized as a single insert and cloned into a hairpin configuration in a binary vector, which was used to transform TYLCV-susceptible tomato plants. Eight of 28 independent transgenic tomato lines exhibited immunity to TYLCV-Is and to TYLCV-Mld, but not to tomato yellow leaf curl Sardinia virus, which shares relatively low sequence homology with the transgene. In addition, a marker-free (nptII-deleted) transgenic tomato line was generated for the first time by Agrobacterium-mediated transformation without antibiotic selection, followed by screening of 1180 regenerated shoots by whitefly-mediated TYLCV inoculation. Resistant lines showed a high level of transgene-siRNA (t-siRNA) accumulation (22% of total small RNA) with dominant sizes of 21 nt (73%) and 22 nt (22%). The t-siRNA displayed hot-spot distribution ("peaks") along the transgene, with different distribution patterns than the viral-siRNA peaks observed in TYLCV-infected tomato. A grafting experiment demonstrated the mobility of 0.04% of the t-siRNA from transgenic rootstock to non-transformed scion, even though scion resistance against TYLCV was not achieved.

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Section: Size and Distribution Of T-sirna In Transgenic Rootstock Andmentioning

confidence: 84%

Section: Size and Distribution Of T-sirna In Transgenic Rootstock Andmentioning

confidence: 99%

Section: Accumulation Of T-sirna In Transgenic Tomato Linesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

et al. 2015

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“…The use of pathogen-derived resistance, whereby a portion of the virus genome is incorporated into the plant genome, has been successfully used for a range of plant viruses (Cillo and Palukaitis 2014). This approach has been recently used to engineer resistance in sunflower against a strain of TSV from India (Pradeep et al 2012).…”

Section: Use Of Genetic Resistancementioning

confidence: 99%

Epidemiology and genetic diversity of Tobacco streak virus and related subgroup 1 ilarviruses

Sharman¹

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A quarter of Australia's sunflower production is from the central highlands region of Queensland and is currently worth six million dollars ($AUD) annually. From the early 2000s a severe necrosis disorder of unknown aetiology was affecting large areas of sunflower crops in central Queensland, leading to annual losses of up to 20%. Other crops such as mung bean and cotton were also affected. This PhD study was undertaken to determine if the causal agent of the necrosis disorder was of viral origin and, if so, to characterise its genetic diversity, biology and disease cycle, and to develop effective control strategies.The research described in this thesis identified Tobacco streak virus (TSV; genus Ilarvirus, family Bromoviridae) as the causal agent of the previously unidentified necrosis disorder of sunflower in central Queensland. TSV was also the cause of commonly found diseases in a range of other crops in the same region including cotton, chickpea and mung bean. This was the first report from Australia of natural field infections of TSV from these four crops.TSV strains have previously been reported from other regions of Australia in several hosts based on serological and host range studies. In order to determine the relatedness of previously reported TSV strains with TSV from central Queensland, we characterised the genetic diversity of the known TSV strains from Australia. We identified two genetically distinct TSV strains from central Queensland and named them based on their major alternative hosts, TSV-parthenium from Parthenium hysterophorus and TSV-crownbeard from Verbesina encelioides. They share only 81 % totalgenome nucleotide sequence identity. In addition to TSV-parthenium and TSV-crownbeard from central Queensland, we also described the complete genomes of two other ilarvirus species. This proved that previously reported TSV strains, TSV-S isolated from strawberry and TSV-Ag from Ageratum houstonianum, were actually the first record of Strawberry necrotic shock virus from Australia, and a new subgroup 1 ilarvirus, Ageratum latent virus. Our results confirmed that the TSV strains found in central Queensland were not related to previously described strains from Australia and may represent new incursions. This is the first report of the genetic diversity within subgroup 1 ilarviruses from Australia.Based on field observations we hypothesised that parthenium and crownbeard were acting as symptomless hosts of TSV-parthenium and TSV-crownbeard, respectively. We developed strainspecific multiplex PCRs for the three RNA segments to accurately characterise the range of naturally infected hosts across central Queensland. Results described in this thesis show compelling evidence that parthenium and crownbeard are the major (symptomless) alternative hosts of TSV-4 parthenium and TSV-crownbeard. While both TSV strains had wide natural host ranges, the geographical distribution of each strain was closely associated with the respective distribution of their major alternative hosts. Both TSV strains were ...

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Diseases of Cassava

Igwe

2023

Handbook of Vegetable and Herb Diseases

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Transgenic Resistance

Cited by 57 publications

References 576 publications

Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

Epidemiology and genetic diversity of Tobacco streak virus and related subgroup 1 ilarviruses

Diseases of Cassava

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