Transgenic rice expressing Allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests

Saha, Prasenjit; Majumder, Pralay; Dutta, Indrajit; Ray, Tui; Roy, Swarup; Das, Sampa

doi:10.1007/s00425-005-0182-z

Cited by 130 publications

(79 citation statements)

References 62 publications

(81 reference statements)

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“…Concerns about possible consequences to higher animals of ingesting snowdrop lectin have limited further progress, although a recent study incorporating a 90-d feeding trial found no adverse effects resulting from consumption of transgenic rice expressing GNA by rats (Poulsen et al, 2007). Similar partial resistance to hemipterans has also been obtained by expression of a Man-specific lectin from garlic (Allium sativum) leaves (ASA-L) in transgenic rice (Saha et al, 2006a) and a variety of other transgenic plant species. The transgenic rice plants expressing ASA-L were shown to decrease transmission of Rice tungro virus by its insect vector, presumably as a result of decreased feeding by the pest (Saha et al, 2006b).…”

Section: Lectins and Sap-sucking Pestsmentioning

confidence: 99%

Biotechnological Prospects for Engineering Insect-Resistant Plants

Gatehouse

2008

Plant Physiology

194

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Insect-resistant crops have been one of the major successes of applying plant genetic engineering technology to agriculture; cotton (Gossypium hirsutum) resistant to lepidopteran larvae (caterpillars) and maize (Zea mays) resistant to both lepidopteran and coleopteran larvae (rootworms) have become widely used in global agriculture and have led to reductions in pesticide usage and lower production costs (Toenniessen et al., 2003;Brookes and Barfoot, 2005).The source of the insecticidal toxins produced in commercial transgenic plants is the soil bacterium Bacillus thuringiensis (Bt). Bt strains show differing specificities of insecticidal activity toward pests, and constitute a large reservoir of genes encoding insecticidal proteins, which are accumulated in the crystalline inclusion bodies produced by the bacterium on sporulation (Cry proteins, Cyt proteins) or expressed during bacterial growth (Vip proteins). The threedomain Cry proteins have been extensively studied; their mechanism of action involves a proteolytic activation step, which occurs in the insect gut after ingestion, followed by interaction of one or both of domains II and III with ''receptors'' on the surface of cells of the insect gut epithelium. This interaction leads to oligomerization of the protein, and domain I is then responsible for the formation of an open channel through the cell membrane . The resulting ionic leakage destroys the cell, leading to breakdown of the gut, bacterial proliferation, and insect death.However, not all pests are adequately targeted by the Bt toxins used at present, and there is still a need to develop solutions to specific problems, such as resistance to sap-sucking pests and pests of stored products. This Update will review some developments to the basic Bt strategy and selected alternative methods for engineering insect resistance.

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Section: Lectins and Sap-sucking Pestsmentioning

confidence: 99%

Biotechnological Prospects for Engineering Insect-Resistant Plants

Gatehouse

2008

Plant Physiology

194

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“…A few examples of transgenes for pest protection that have already been used in rice transformation and could become available in Vietnam and elsewhere include Bt and cowpea trypsin inhibitor genes for resistance to lepidopteran larvae (High et al, 2004;Huang et al, 2005), lectin genes for resistance to sucking insects (Nagadhara et al, 2003;Saha et al, 2006), chitinase or allene oxide synthase genes for resistance to fungal diseases (Datta et al, 2001;Mei et al, 2006), and trangenes for resistance to bacterial blight (Zai et al, 2004). Other transgenic traits that could be released separately or in combination with various types of pest resistance include resistance to commonly used herbicides, greater tolerance of abiotic stresses, and enhanced production of pro-vitamin A (e.g., Lu and Snow, 2005).…”

Section: 15mentioning

confidence: 99%

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Cohen

Arpaia

Lan

et al. 2008

Environ. Biosafety Res.

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Many varieties of transgenic rice are under development in countries where wild and weedy relatives co-occur with the crop. To evaluate possible risks associated with pollen-mediated transgene dispersal, we conducted a two-year survey in Vietnam to examine overlapping flowering periods of rice (Oryza sativa L.), weedy rice (O. sativa), and wild rice (O. rufipogon Griff.), all of which are inter-fertile. We surveyed populations in two regions of the Mekong Delta, northern and southern, and at three sites in each of three habitats per region: fresh water, saline water, and acid sulfate soil. Weedy rice frequently flowered simultaneously with neighboring cultivated rice plants. Flowering was more seasonal in wild rice and often peaked in November and December. Peak flowering times of wild rice overlapped with adjacent rice fields at all of the saline sites and half of the acid sulfate sites. The longer flowering season of wild rice ensured that crop-to-wild gene flow was possible in fresh water habitats as well. Our second objective was to determine whether wild and weedy rice populations are exposed to pests that could be targeted by future transgenes, which may then provide fitness benefits. These populations shared many pathogen and insect herbivore species with cultivated rice (leaffolder, locust, cricket, planthoppers, rice bug, stem borer, sheath blight, blast, bacterial leaf blight, and brown spot). Damage by leaffolders and locusts was the most frequently observed insect feeding damage on all three rice types. Indicator species analysis revealed that most of the insect herbivores were associated with particular habitats, demonstrating the importance of broad geographic sampling for transgenic rice risk assessment. These survey data and the strong likelihood of gene flow from cultivated rice suggest that further studies are needed to examine the effects of transgenic traits such as resistance to pests on the abundance of wild and weedy rice.

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“…Some lectins, such as the garlic bulb lectin, play a dual role as storage protein and as defense proteins that are mobilized against attacking insects Smeets et al, 1997;Dinant et al, 2003). Transgenic rice (Oryza sativa) plants expressing garlic leaf agglutinin have antifeedant properties that are insecticidal against sap-sucking insect pests such as the brown planthopper, the green leafhopper (Nephotettix virescens; Saha et al, 2006), and the Egyptian cotton leafworm (Sadeghi et al, 2008).…”

mentioning

confidence: 99%

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

et al. 2008

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We previously cloned and characterized a novel jacalin-like lectin gene from wheat (Triticum aestivum) plants that responds to infestation by Hessian fly (Mayetiola destructor) larvae, a major dipteran pest of this crop. The infested resistant plants accumulated higher levels of Hfr-1 (for Hessian fly-responsive gene 1) transcripts compared with uninfested or susceptible plants. Here, we characterize the soluble and active recombinant His 6 -HFR1 protein isolated from Escherichia coli. Functional characterization of the protein using hemagglutination assays revealed lectin activity. Glycan microarray-binding assays indicated strong affinity of His 6 -HFR1 to Mana1-6(Mana1-3)Man trisaccharide structures. Resistant wheat plants accumulated high levels of HFR1 at the larval feeding sites, as revealed by immunodetection, but the avirulent larvae were deterred from feeding and consumed only small amounts of the lectin. Behavioral studies revealed that avirulent Hessian fly larvae on resistant plants exhibited prolonged searching and writhing behaviors as they unsuccessfully attempted to establish feeding sites. During His 6 -HFR1 feeding bioassays, Drosophila melanogaster larvae experienced significant delays in growth and pupation, while percentage mortality increased with progressively higher concentrations of His 6 -HFR1 in the diet. Thus, HFR1 is an antinutrient to dipteran larvae and may play a significant role in deterring Hessian fly larvae from feeding on resistant wheat plants.

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Transgenic rice expressing Allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests

Cited by 130 publications

References 62 publications

Biotechnological Prospects for Engineering Insect-Resistant Plants

Biotechnological Prospects for Engineering Insect-Resistant Plants

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

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