Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant

Abstract

We introduced the potato proteinase inhibitor II (PINII) gene (pin2) into several Japonica rice varieties, and regenerated a large number of transgenic rice plants. Wound-inducible expression of the pin2 gene driven by its own promoter, together with the first intron of the rice actin 1 gene (act1), resulted in high-level accumulation of the PINII protein in the transgenic plants. The introduced pin2 gene was stably inherited in the second, third, and fourth generations, as shown by molecular analyses. Based on data from the molecular analyses, several homozygous transgenic lines were obtained. Bioassay for insect resistance with the fifth-generation transgenic rice plants showed that transgenic rice plants had increased resistance to a major rice insect pest, pink stem borer (Sesamia inferens). Thus, introduction of an insecticidal proteinase inhibitor gene into cereal plants can be used as a general strategy for control of insect pests.

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

“…Transgenic study of soybean Kunitz trypsin inhibitors (TIs) in tobacco have show promising results against H. virescence (Lepidoptera) (Gatehouse et al 1993). Transgenic study of PIs from other plants has also done, for example, (1) potato PI-2 expressed in tobacco (McManus et al 1994;Jongsma et al 1995) and in rice (Duan et al 1996) which were investigated for deleterious effects on lepidopterans Manduca sexta (Johnson et al 1989), Spodoptera litura (McManus et al 1994), Spodoptera exigua (Jongsma et al 1995), and Sesamia inferens (Duan et al 1996); (2) oryzacystatin I from rice expressed in tomato (Urwin et al 1995), poplar (Leple et al 1995), potato (Cloutier et al 1999(Cloutier et al , 2000, and oilseed rape BonadeBottino et al 1999) for effect against Coleopteran pests.…”

Gene dosage and genetic background affect miraculin accumulation in transgenic tomato fruits

“…Many factors can contribute to the variation in transgene expression. Studies in rice showed that transgene expression levels were affected by gene dosage (Baruah-Wolff et al 1999;Duan et al 1996). Scott et al (1998) have claimed that allelic composition and genetic background affect transgene expression and inheritance in white clover.…”

Transient expression of - glucuronidase gene in indica and japonica rice (Oryza sativa L.) callus cultures after different stages of co-bombardment

“…Resistance to antibiotic hygromycin B has been used as selection criterion for selecting transformed cells in this study as reported previously in developing transgenic rice (Lin et al, 1995;Sivamani et al, 1996;Duan et al, 1996;Zhang et al, 1996;Wu et al, 1997;Chen et al, 1998b;Abedinia et al, 2004). Sensitivity of calli to hygromycin was assessed before transformation experiments.…”

“…E-Mail: ramesh_biotek@yahoo.co.in japonica cultivars (Christou et al, 1991;Cao et al,1992;Li et al,1993;Duan et al,1996;), indica cultivars (Li et al,1993;Sivamani et al, 1996;Zhang et al,1996;Jain et al, 1996;Ghosh Biswas et al, 1998), elite cultivars belong to Central America and West Africa (Valdez et al,1998), U.S. rice lines (Jiang et al ., 2000) and Australian rice cultivars (Abedinia et al, 2004) have been successfully used as target tissue to develop transgenic plants expressing various traits. Earlier, optimization of various parameters for the introduction of -glucuronidase gene into embryogenic callus cultures of indica rice variety Basmati 370 (Minhas et al, 1996) and suspension cells of Pusa Basmati 1 (Jain et al, 1996) have been reported.…”