Transgenic rice plants that over-express the mannose-binding rice lectin have enhanced resistance to rice blast

Shinjo, Akira; Araki, Yuko; Hirano, Ken; Arie, Tsutomu; Ugaki, Masashi; Teraoka, Tohru

doi:10.1007/s10327-010-0290-x

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…Recently, this gene was identified in Saltol-1, a major quantitative trait loci related to salt stress [ 139 ]. Overexpression of Orysata , also known as SALT, in rice plants improves salinity tolerance [ 140 ] and enhances resistance to Magnaporthe grisea infection [ 141 ]. The Arabidopsis-related JAX1 was shown to confer broad but specific resistance to potex viruses [ 142 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Holle

Schutter

Eggermont

et al. 2017

IJMS

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Lectins are present throughout the plant kingdom and are reported to be involved in diverse biological processes. In this study, we provide a comparative analysis of the lectin families from model species in a phylogenetic framework. The analysis focuses on the different plant lectin domains identified in five representative core angiosperm genomes (Arabidopsis thaliana, Glycine max, Cucumis sativus, Oryza sativa ssp. japonica and Oryza sativa ssp. indica). The genomes were screened for genes encoding lectin domains using a combination of Basic Local Alignment Search Tool (BLAST), hidden Markov models, and InterProScan analysis. Additionally, phylogenetic relationships were investigated by constructing maximum likelihood phylogenetic trees. The results demonstrate that the majority of the lectin families are present in each of the species under study. Domain organization analysis showed that most identified proteins are multi-domain proteins, owing to the modular rearrangement of protein domains during evolution. Most of these multi-domain proteins are widespread, while others display a lineage-specific distribution. Furthermore, the phylogenetic analyses reveal that some lectin families evolved to be similar to the phylogeny of the plant species, while others share a closer evolutionary history based on the corresponding protein domain architecture. Our results yield insights into the evolutionary relationships and functional divergence of plant lectins.

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Section: Resultsmentioning

confidence: 99%

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Holle

Schutter

Eggermont

et al. 2017

IJMS

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“…Interestingly, strong support of resistance to sheath blight was evident from the growth suppression and reduction in the number of infection cushions observed in the leaf surface of transgenic lines. Earlier reports also showed suppression of fungal invasive hyphae in transgenic rice expressing antifungal proteins [ 35 , 36 ]. Definitive proof of sheath blight resistance came from the whole plant bioassay, which was performed according to Park et al [ 37 ].…”

Section: Discussionmentioning

confidence: 90%

Monitoring the efficacy of mutated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani

et al. 2016

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BackgroundRice sheath blight, caused by Rhizoctonia solani is one of the most devastating diseases of rice. It is associated with significant reduction in rice productivity worldwide. A mutant variant of mannose binding Allium sativum leaf agglutinin (mASAL) was previously reported to exhibit strong antifungal activity against R. solani. In this study, the mASAL gene has been evaluated for its in planta antifungal activity in rice plants.ResultsmASAL was cloned into pCAMBIA1301 binary vector under the control of CaMV35S promoter. It was expressed in an elite indica rice cv. IR64 by employing Agrobacterium tumefaciens-mediated transformation. Molecular analyses of transgenic plants confirmed the presence and stable integration of mASAL gene. Immunohistofluorescence analysis of various tissue sections of plant parts clearly indicated the constitutive expression of mASAL. The segregation pattern of mASAL transgene was observed in T1 progenies in a 3:1 Mendelian ratio. The expression of mASAL was confirmed in T0 and T1 plants through western blot analysis followed by ELISA. In planta bioassay of transgenic lines against R. solani exhibited an average of 55 % reduction in sheath blight percentage disease index (PDI).ConclusionsThe present study opens up the possibility of engineering rice plants with the antifungal gene mASAL, conferring resistance to sheath blight.

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“…n.d., not detected. (Garcia et al, 1998;De Souza Filho et al, 2003;Shinjo et al, 2011;Al Atalah et al, 2014). Similar to OsEULs, Orysata shows expression in roots and shoots.…”

Section: Discussionmentioning

confidence: 95%

OsEUL Lectin Gene Expression in Rice: Stress Regulation, Subcellular Localization and Tissue Specificity

et al. 2020

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The Euonymus lectin (EUL) family is a unique group of carbohydrate-binding proteins that is omnipresent in plants. Sequences encoding EUL-related lectins have been retrieved from all completely sequenced plant genomes. The rice (Oryza sativa) genome contains 5 functional EUL genes referred to as OsEULS2, OsEULS3, OsEULD1a, OsEULD1b, and OsEULD2. In this study we focused on the tissue specific expression, stress inducibility and subcellular localization of the rice EULs. Even though the EUL domain sequence is highly conserved among the rice EULs (at least 80% sequence similarity) different biotic and abiotic stress treatments yielded unique responses for the different EULs. Transcript levels for OsEULs were differentially affected by drought and salt stress, ABA treatment, pathogen infection or insect infestation. Analysis of promoter activity revealed differential expression and tissue specificity for the 5 OsEUL genes, with most expression observed in the vascular system of roots and shoots, as well as in the root tips and seeds. At cell level, all OsEULs are located in the nucleus whereas OsEULD1b and OsEULD2 also locate to the cytoplasm. This paper contributes to the functional characterization of the EULs and provides insight in the biological importance of this family of proteins for rice.

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Transgenic rice plants that over-express the mannose-binding rice lectin have enhanced resistance to rice blast

Cited by 9 publications

References 34 publications

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Monitoring the efficacy of mutated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani

OsEUL Lectin Gene Expression in Rice: Stress Regulation, Subcellular Localization and Tissue Specificity

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