Transgenic RNAi of BmREEPa in silkworms can enhance the resistance of silkworm to Bombyxmori Nucleopolyhedrovirus

Dong, Xinxin; Liu, Tai-hang; Wang, Wei; Pan, Cai-Xia; Du, Guo-yu; Wu, Yunfei; Adur, Malavika K; Zhang, Min-Juan; Pan, Min‐Hui; Lü, Cheng

doi:10.1016/j.bbrc.2017.01.017

Cited by 8 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several approaches for identifying the interaction between viruses and hosts. Feng et al obtained the protein SINAL10, which interacts with the baculovirus capsid protein GP64, by using a yeast two-hybrid and baculovirus library screening method [ 25 ], while Dong et al obtained the BmREEPa protein by proteomic differential analysis of two cell lines, BmN-SWU1 and BmN-SWU2, which interacts with GP64 protein through the BmPTCHD protein [ 27 ]. In the present study, BmREEPa protein was also obtained by screening in cells and silkworm samples.…”

Section: Discussionmentioning

confidence: 99%

“…The invasion and replication mechanisms of BmNPV remain largely unknown, and in particular, the investigation of its receptor protein is warranted. Recently, some proteins were shown to be associated with the invasion and replication of BmNPV, including TRAP1 protein, a member of the heat shock protein 90 family [ 24 ], E3 ubiquitin-protein ligase SINA-like 10 (SINAL10) [ 25 ], B. mori nuclear hormone receptor 96 (BmNHR96) [ 26 ], and B. mori receptor expression-enhancing protein (BmREEPa) [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

Wang

Ren

et al. 2022

Insects

View full text Add to dashboard Cite

Virus–host interactions are critical for virus replication, virulence, and pathogenicity. The Bombyx mori nucleopolyhedrovirus (BmNPV) is a typical model baculovirus, representing one of the most common and harmful pathogens in sericulture. Herein, we used co-immunoprecipitation to identify candidate proteins with potential interactions with BmNPV. First, a recombinant BV virus particle rBmBV-egfp-p64-3×flag-gp64sp was constructed using a MultiBac baculovirus multigene expression system. Co-immunoprecipitation experiments were then performed with the recombinant BV virus infected with BmN cells and Dazao silkworms. LC-MS/MS analysis revealed a total of 845 and 1368 candidate proteins were obtained from BmN cells and silkworm samples, respectively. Bioinformatics analysis (Gene Ontology, KEGG Pathway) was conducted for selection of proteins with significant enrichment for further confirmation of the effects on BmNPV replication. Overall, the results showed that SEC61 and PIC promoted the replication of BmNPV, while FABP1 inhibited the replication of BmNPV. In summary, this study reveals the potential proteins involved in BmNPV invasion and proliferation in the host and provides a platform for identifying the potential receptor proteins of BmNPV.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

Wang

Ren

et al. 2022

Insects

View full text Add to dashboard Cite

show abstract

“…However, baculoviruses BmNPV have developed several strategies to evade the host's immune system and promote their own replication and proliferation, Viruses 2022, 14, 2636 2 of 14 including the inhibition of antiviral melanization, autophagy, apoptosis, RNAi, and regulation of the cell cycle. In the past decades, many cellular proteins that promote BmNPV proliferation have been identified, including Bombyx mori nuclear hormone receptor 96 (BmNHR96) [2], Bombyx mori receptor expression-enhancing protein (BmREEPa) [3], Bombyx mori E3 ubiquitin-protein ligase SINA-like 10 (BmSINAL10) [4], and the heat shock protein (HSP) family [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Overexpression Bombyx mori HEXIM1 Facilitates Immune Escape of Bombyx mori Nucleopolyhedrovirus by Suppressing BmRelish-Driven Immune Responses

Chen

Kong

et al. 2022

Viruses

View full text Add to dashboard Cite

Bombyx mori nucleopolyhedrovirus (BmNPV), a typical arthropod-specific enveloped DNA virus, is one of the most serious pathogens in silkworm farming, but the potential mechanisms of the evasion of innate immune responses from BmNPV infection are still poorly understood. HEXIM1 is an RNA-binding protein, best known as an inhibitor of positive transcription elongation factor b (P-TEFb), which controls transcription elongation by RNA polymerase II. In this study, Bombyx mori HEXIM1 (BmHEXIM1) was cloned and characterized, and its expression was found to be remarkably upregulated after BmNPV infection. Furthermore, BmHEXIM1 was detected to increase the proliferation of BmNPV, and its full length is essential for assisting BmNPV immune escape by suppressing BmRelish-driven immune responses. This study brought new insights into the mechanisms of immune escape of BmNPV and provided theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.

show abstract

In vivo RNA interference of BmNHR96 enhances the resistance of transgenic silkworm to BmNPV

Yang

Liu

Dong

et al. 2017

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Transgenic RNAi of BmREEPa in silkworms can enhance the resistance of silkworm to Bombyxmori Nucleopolyhedrovirus

Cited by 8 publications

References 20 publications

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

Overexpression Bombyx mori HEXIM1 Facilitates Immune Escape of Bombyx mori Nucleopolyhedrovirus by Suppressing BmRelish-Driven Immune Responses

In vivo RNA interference of BmNHR96 enhances the resistance of transgenic silkworm to BmNPV

Contact Info

Product

Resources

About