Transgenic tobacco co-expressing flavodoxin and betaine aldehyde dehydrogenase confers cadmium tolerance through boosting antioxidant capacity

Shahbazi, Mehrdad; Tohidfar, Masoud; Aliniaeifard, Sasan; Yazdanpanah, Farzaneh; Bosacchi, Massimo

doi:10.1007/s00709-021-01714-1

Cited by 6 publications

(3 citation statements)

References 98 publications

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“…In contrast to the control, L. obtusifolium showed significantly lower growth and biomass accumulation when soil Zn was increased. As Zn concentration increased, the plant biomass increased and then decreased in Sinapis alba L. and Sasa argenteostriata (Regel) [50,51]. The growth of citrus plants was also inhibited by excessive Zn stress.…”

Section: Effect Of Different Trace Elements On the Growth Of L Obtusi...mentioning

confidence: 99%

“…ROS include superoxide radicals, hydroxyl radicals, and hydrogen peroxide radicals [49]. Plants are affected by ROS that can damage their membranes, proteins, and nucleic acids [51]. Plants are activated by their antioxidant enzyme system to resist external adverse environmental conditions and maintain their normal growth when they are under stress.…”

Section: Antioxidant Enzyme Response To Trace Element Stress In L Obt...mentioning

confidence: 99%

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Effects of Different Trace Elements on Ecophysiological Characteristics of Ligustrum obtusifolium Saplings

Cao

Arif

Cui

et al. 2023

Forests

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Phytoremediation is becoming more prevalent globally. Literature on phytoremediation strategies in western China is relatively scarce. The present research sought to fill this gap by examining the effects of trace elements such as Cd, Cr, Pb, and Zn on growth, physiological traits, tolerances, and accumulation characteristics in 2-year-old saplings of Ligustrum obtusifolium. The gradient of trace element concentration was determined by adding exogenous trace elements to the soil in a pot experiment: CK (no exogenous trace element), T1, T2, and T3 (Cd 0, 2, 5, and 10 mg/kg; Cr 0, 300, 500, and 700 mg/kg; Pb 0, 400, 800, and 1200 mg/kg; and Zn 0, 300, 500, and 1000 mg/kg, respectively). The results indicated that Chla, Chlb, and total Chls significantly decreased (p < 0.05) in the leaves of L. obtusifolium, with the lowest value obtained during soil treatment with T3. Along with the increase in trace element concentration, the net increase in height, root biomass, aerial biomass, and total biomass was reduced significantly. The net growth of L. obtusifolium under Cr stress did not differ significantly from that of CK at T1; however, the net growth of L. obtusifolium under Cr stress was considerably reduced at T2 and T3. The antioxidant enzyme activity of L. obtusifolium increased under different trace element stresses and first increased and then decreased as trace element levels increased. It was found that the SOD, POD, CAT, and APX activity of L. obtusifolium peaks at T2 under Cd and Zn stress in contrast to a peak at T1 under Cr and Pb stress. The contents of trace elements in L. obtusifolium roots, stems, and leaves increased along with the increase in soil trace element levels. Cr, Pb, and Zn threshold values can be set at 300 mg/kg, 400 mg/kg, and 300 mg/kg, respectively. This is carried out by using a reference index of the biomass of L. obtusifolium decreased by 10%, while the Cd threshold value needs to be further studied. L. obtusifolium would be an appropriate plant for phytoremediation of Cr-polluted soil, compared to Cd, Pb, and Zn absorption and accumulation. It showed optimal antioxidant enzyme activity and transfer ability under soil Cr contents of 300 mg/kg, and the growth of L. obtusifolium was not restricted. Therefore, L. obtusifolium was particularly suitable for phytoremediation of Cr pollution in areas in western China.

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Section: Effect Of Different Trace Elements On the Growth Of L Obtusi...mentioning

confidence: 99%

Section: Antioxidant Enzyme Response To Trace Element Stress In L Obt...mentioning

confidence: 99%

Effects of Different Trace Elements on Ecophysiological Characteristics of Ligustrum obtusifolium Saplings

Cao

Arif

Cui

et al. 2023

Forests

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“…It is a quaternary ammonium compound that accumulates in various organisms adapted to arid or high-salt environments, and can enhance plant tolerance to various abiotic stresses [23][24][25]. However, tobacco cannot synthesize GB by itself and GB can be accumulated in the tobacco by the genetic engineering of the GB synthase gene, so the stress resistance of tobacco can thus be effectively improved [26][27][28]. This study used a transgenic tobacco line carrying the spinach betaine aldehyde dehydrogenase (BADH) gene to explore the maintenance and protective mechanism of GB on crop photosynthesis under drought stress.…”

Section: Introductionmentioning

confidence: 99%

Transgenic Tobacco with the BADH Gene Shows Enhanced Photosynthesis Resistance to Drought Stress Induced by PEG-6000

Wang,

Yang,

Xue

2024

Agronomy

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Extreme weather events, including drought, have occurred worldwide with increasing frequency and severity in recent years. Drought stress is the main limiting factor for agricultural development in many regions, including tobacco—an important economic crop and a model plant for scientific research. As it is adapted to the tropics, tobacco is highly susceptible to drought stress, with resultant decreases in yield and quality. Glycine betaine (GB) is an osmoregulatory substance that can enhance plant resistance to various abiotic stresses. Here, we investigate the protective mechanism of genetically engineered glycine betaine (GB) on tobacco photosynthesis under drought stress induced by 30% PEG-6000. This study used transgenic tobacco (T) accumulating GB and wild-type tobacco (WT) to investigate the protective effects conferred by the genetic engineering of GB synthesis on tobacco photosynthesis under drought stress (induced by 30% PEG-6000). The results showed that the net photosynthetic rate of the tobacco plants significantly decreased under drought stress, and the degree of decrease was significantly lower in the T line than in the WT line. GB accumulation improved the resistance of photosynthesis to drought stress. Furthermore, under drought stress, the photosynthesis improvement in the T line was related to the accumulation of GB, leading to maintenance of the water status, the promotion of osmotic regulation, and an enhancement in antioxidant enzyme activities, which reduced membrane peroxidation and thereby increased the thylakoid membrane’s protein content and function, especially the photosystem II (PSII) function. The results provide a theoretical basis for further research on genetic engineering related to GB synthesis and the field application of exogenous GB.

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Synergistic interactions of assorted ameliorating agents to enhance the potential of heavy metal phytoremediation

Sanjana,

Jazeel,

Janeeshma

et al. 2024

Stress Biology

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Pollution by toxic heavy metals creates a significant impact on the biotic community of the ecosystem. Nowadays, a solution to this problem is an eco-friendly approach like phytoremediation, in which plants are used to ameliorate heavy metals. In addition, various amendments are used to enhance the potential of heavy metal phytoremediation. Symbiotic microorganisms such as phosphate-solubilizing bacteria (PSB), endophytes, mycorrhiza and plant growth-promoting rhizobacteria (PGPR) play a significant role in the improvement of heavy metal phytoremediation potential along with promoting the growth of plants that are grown in contaminated environments. Various chemical chelators (Indole 3-acetic acid, ethylene diamine tetra acetic acid, ethylene glycol tetra acetic acid, ethylenediamine-N, N-disuccinic acid and nitrilotri-acetic acid) and their combined action with other agents also contribute to heavy metal phytoremediation enhancement. With modern techniques, transgenic plants and microorganisms are developed to open up an alternative strategy for phytoremediation. Genomics, proteomics, transcriptomics and metabolomics are widely used novel approaches to develop competent phytoremediators. This review accounts for the synergistic interactions of the ameliorating agent’s role in enhancing heavy metal phytoremediation, intending to highlight the importance of these various approaches in reducing heavy metal pollution.

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Transgenic tobacco co-expressing flavodoxin and betaine aldehyde dehydrogenase confers cadmium tolerance through boosting antioxidant capacity

Cited by 6 publications

References 98 publications

Effects of Different Trace Elements on Ecophysiological Characteristics of Ligustrum obtusifolium Saplings

Effects of Different Trace Elements on Ecophysiological Characteristics of Ligustrum obtusifolium Saplings

Transgenic Tobacco with the BADH Gene Shows Enhanced Photosynthesis Resistance to Drought Stress Induced by PEG-6000

Synergistic interactions of assorted ameliorating agents to enhance the potential of heavy metal phytoremediation

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