Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen

Yu, Tao; Li, Yang Sheng; Chen, Xue Feng; Hu, Jing; Chang, Xulu; Zhu, Ying

doi:10.1078/0176-1617-01205

Cited by 70 publications

(45 citation statements)

References 35 publications

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“…Simultaneously, the crude pET-32A without GhGST gene protein had low GST activity, similar to that previously reported (Yu et al, 2003).…”

Section: Discussionsupporting

confidence: 78%

“…The GhGST in this study was different from the previously reported GST cDNA fragment expressed in tobacco (Yu et al, 2003). Although any sequence information was not mentioned, the GST cDNA fragment cloned into pET28A with the HindIII site indicated that there was no HindIII site in encoding region of the GST cDNA fragment.…”

Section: Discussionmentioning

confidence: 80%

“…A so-called cotton GST cDNA fragment without HindIII site in the translation region was transferred to tobacco, and the transgenic plants showed the enhanced resistance to methyl viologen (Yu et al, 2003), but no more information of the gene sequence and amino acids were reported. In present study, we cloned a tau Glutathione S-transferase subunit encoding gene from cotton (Gossypium hirsutum).…”

Section: Introductionmentioning

confidence: 99%

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Cloning and characterization of a tau glutathione S-transferase subunit encoding gene in Gossypium hirsutum

Wang

et al. 2008

Genes Genet. Syst.

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A predicted tau Glutathione S-transferase (GST) subunit encoding gene, named GhGST, was isolated from Gossypium hirsutum with RACE method from SSH library based on Verticillium dahliae stress. The data revealed an open reading frame of 678 bp encoding a protein of 225 amino acids with a molecular weight of 25.821 kDa. Semi-quantitative RT-PCR analysis showed that the mRNA of GhGST was expressed in root, stem and leaf. And the content of GhGST expression increased under Verticillium dahliae stress in root. The expression of GhGST gene was verified by transformation in E. coli BL21 (DE3) strain with the recombinant expression vector pET-32A. GST activity assay showed the crude GhGST protein had obvious activity to 1-chloro-2,4-dinitrobenzene (CDNB) substrate.

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“…Simultaneously, the crude pET-32A without GhGST gene protein had low GST activity, similar to that previously reported (Yu et al, 2003).…”

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Cloning and characterization of a tau glutathione S-transferase subunit encoding gene in Gossypium hirsutum

Wang

et al. 2008

Genes Genet. Syst.

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“…For example, transgenic wheat plants expressing the maize GST-27 gene were resistant to chloroacetanilide herbicide alachlor and dimethenamid, and thiocarbamate herbicide S-ethyldipropylthio-carbamate (Milligan et al 2001). Transgenic tobacco plants expressing the cotton Gst-cr1 gene showed much higher expression levels of GST and GPX activities, and showed an enhanced resistance to oxidative stress induced by a low concentration of methyl viologen (Yu et al 2003). Transgenic tobacco plants expressing maize glutathione S-transferase I presented substantially higher tolerance to alachlor compared to non-transgenic plants (Karavangeli et al 2005).…”

Section: Transgenic Rice Has Higher Tolerance To Herbicidementioning

confidence: 99%

A glutathione s-transferase confers herbicide tolerance in rice

2014

Crop Breed. Appl. Biotechnol.

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“…However, certain tau class GSTs possess a glutathione-peroxidase activity and are able to catalyze the breakdown of H 2 O 2 and lipid hydroperoxides (Bartling et al, 1993;Dixon et al, 2009b). In fact, overexpression of GSTs with both GST and GPX activity conferred enhanced tolerance of tobacco to paraquat and thermal or salt stress (Roxas et al, 1997;Yu et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis

Viczián¹,

Künstler²,

Hafez³

et al. 2014

Acta Phytopathologica et Entomologica Hungarica

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High concentrations of the reactive oxygen species (ROS) superoxide (O 2•-) and hydrogen peroxide (H 2 O 2 ) contribute to the induction of plant cell and tissue death (necrosis). In an effort to create transgenic plants with high antioxidant capacity that could resist necrotic symptoms we produced two transgenic tobacco (Nicotiana tabacum cv. SR1) lines (S1 and S2) overexpressing a tomato chloroplast superoxide dismutase (SlChSOD). SOD genes encode for antioxidant enzymes that dismutate superoxide to hydrogen peroxide. Therefore, SOD-overproducing plants may contain high levels of hydrogen peroxide and are sensitive to stress-related necrosis unless sufficient degradation of hydrogen peroxide is conferred by elevated expression of antioxidants like e.g. catalases and peroxidases. Indeed, line S1 displayed elevated expression of a glutathione peroxidase (NtGPX) and a glutathione S-transferase (NtGSTU1b), as compared to wild type plants. Interestingly, however, expression of a catalase (NtCAT1) was repressed in both SOD-overexpressing lines. This predicts that such plants could be sensitive to localized necrosis (HR) caused by virus infection, since repression of NtCAT1 has been shown to occur during virus-induced HR (e.g. Dorey et al., 1998;Künstler et al., 2007). To elucidate whether other catalases might play a role in resistance to virus induced HR-type necrotic symptoms, a maize catalase (ZmCat2) was transiently overexpressed in Nicotiana edwardsonii and N. edwardsonii var. Columbia plants by agroinfiltration. Inoculation of agroinfiltrated plants with Tobacco mosaic virus (TMV) revealed that ZmCat2 confers enhanced resistance to HR-type necrosis during TMV infection. It seems that catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis.

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Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen

Cited by 70 publications

References 35 publications

Cloning and characterization of a tau glutathione S-transferase subunit encoding gene in Gossypium hirsutum

Cloning and characterization of a tau glutathione S-transferase subunit encoding gene in Gossypium hirsutum

A glutathione s-transferase confers herbicide tolerance in rice

Catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis

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