Transgenic tobacco plants with heat-inducible IAA synthesis genes

Kares, Christa; Depicker, Anna; Debreyne, Peter; Crouzet, Philippe; Otten, Léon

doi:10.1007/978-94-011-2458-4_87

Cited by 1 publication

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Instead, they had pale‐green leaves, epinastic leaf margins, hyponastic petioles and adventitious roots on midveins. In addition, they showed compact growth, inhibition of axillary bud outgrowth, callus formation at the base of seedlings and enhanced lateral root growth (Kares et al ., 1991; Sitbon et al ., 1992). None of these effects have been found in 6b plants.…”

Section: Discussionmentioning

confidence: 99%

New plant growth‐modifying properties of the Agrobacterium T‐6b oncogene revealed by the use of a dexamethasone‐inducible promoter

et al. 2003

View full text Add to dashboard Cite

These authors contributed equally to this work. SummaryAgrobacterium 6b oncogenes induce tumours on Nicotiana glauca and enations and associated modi®ca-tions in transgenic N. tabacum plants. 2x35S-AB-6b tobacco rootstocks produced a graft-transmissible factor that induced enations in wild-type scions; the nature of this enation factor remains to be identi®ed. Here, we report on the properties of tobacco plants carrying a dexamethasone-inducible T-6b gene (dex-T-6b). Induction with dex led to complex growth modi®cations, many of which have not been reported previously. Modi®cations were only found in growing tissues; mature tissues remained unaffected. Growth could be either stimulated or inhibited. Dex induction of young plants led to morphogenetic gradients that included enations, tubular leaves and fragmented leaf primordia. Root elongation was increased or slowed down, while radial root growth was strongly enhanced. Additional cell divisions were found in the root pericycle and vasculature. Enation factor import from mature tissues did not have the same effects on growing tissues as local T-6b synthesis: normal scions grafted on induced dex-T-6b rootstocks formed enations, whereas local dex-T-6b induction at the shoot apex led to numerous dark-green spots on the abaxial side of the leaves. In leaf patch assays, the 23-kDa T-6b protein was found to move through leaves and to enter the vascular system. This and the fact that rootstocks of spontaneous tobacco enation mutants did not modify wild-type scions contrary to 6b plants indicate that the 6b protein might be the enation factor.

show abstract

Section: Discussionmentioning

confidence: 99%