Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development

Pyati, Ujwal J.; Webb, Ashley E.; Kimelman, David

doi:10.1242/dev.01806

Cited by 141 publications

(216 citation statements)

References 52 publications

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“…The mutant lines used were: Tg(hsp70l:dnXla.Bmpr1a-GFP) (Pyati et al, 2005) and Tg(BRE:mRFP) (Wu et al, 2011). Morpholinos (Gene Tools) were injected as described (Wu et al, 2011) at the indicated doses and are listed in supplementary material Table S1.…”

Section: Fish Maintenance and Strainsmentioning

confidence: 99%

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A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border

2013

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SUMMARYDuring ectodermal patterning the neural crest and preplacodal ectoderm are specified in adjacent domains at the neural plate border. BMP signalling is required for specification of both tissues, but how it is spatially and temporally regulated to achieve this is not understood. Here, using a transgenic zebrafish BMP reporter line in conjunction with double-fluorescent in situ hybridisation, we show that, at the beginning of neurulation, the ventral-to-dorsal gradient of BMP activity evolves into two distinct domains at the neural plate border: one coinciding with the neural crest and the other abutting the epidermis. In between is a region devoid of BMP activity, which is specified as the preplacodal ectoderm. We identify the ligands required for these domains of BMP activity. We show that the BMP-interacting protein Crossveinless 2 is expressed in the BMP activity domains and is under the control of BMP signalling. We establish that Crossveinless 2 functions at this time in a positive-feedback loop to locally enhance BMP activity, and show that it is required for neural crest fate. We further demonstrate that the Distal-less transcription factors Dlx3b and Dlx4b, which are expressed in the preplacodal ectoderm, are required for the expression of a cell-autonomous BMP inhibitor, Bambi-b, which can explain the specific absence of BMP activity in the preplacodal ectoderm. Taken together, our data define a BMP regulatory network that controls cell fate decisions at the neural plate border.

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Section: Fish Maintenance and Strainsmentioning

confidence: 99%

“…1A, right panel). We next determined exactly when BMP signalling is required for NC development using a transgenic zebrafish line expressing a dominant-negative BMP type I receptor (dnBMPR1a) in response to heat shock [Tg(hsp70l:dnXla.Bmpr1a-GFP)] (Pyati et al, 2005). This line enabled us to perturb BMP activity at specific stages.…”

Section: Research Articlementioning

confidence: 99%

A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border

2013

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“…Heat-shock-inducible transgenic approaches have provided an invaluable tool through which gene expression in development can be modulated (Halloran et al, 2000;Xiao et al, 2003;Burns et al, 2005;Pyati et al, 2005). To establish if co-injection techniques along with heat-shock-inducible transgenesis could be used to elicite gene expression within established tumors, the rag2-kRASG12D, rag2-GFP and hsp70-dsRED2 constructs were co-injected into one-cell stage AB-strain animals.…”

mentioning

confidence: 99%

Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish

et al. 2008

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The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.

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“…This is largely due to the crucial role of Bmp4 at an early stage of gastrulation (Winnier et al, 1995). In zebrafish, constitutive activation of the Bmp receptor Bmpr1b in the tailbud leads to an expansion of the T-positive mesoderm progenitor zone (Row and Kimelman, 2009), while transgenic expression of a dominant-negative version of the same receptor leads to ectopic tail formation (Pyati et al, 2005).…”

Section: Mesoderm Progenitor Maintenancementioning

confidence: 99%

“…In frog and chick embryos, both gain-and loss-offunction studies identified a role for Bmp4 and Bmpr1 in tailbud outgrowth and somite differentiation (Beck et al, 2001;Reshef et al, 1998;Row and Kimelman, 2009). In zebrafish, loss of Bmp results in ventral fin and tail mesoderm defects (Pyati et al, 2005;Stickney et al, 2007). However, similar comprehensive studies on the later role of Bmp signaling in axis elongation and tail somite formation in mice have been hampered by the essential role of Bmp4 during gastrulation (Winnier et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Bmp signaling maintains a mesoderm progenitor cell state in the mouse tailbud

Sharma¹,

Shafer²,

Bareke³

et al. 2017

Journal of Cell Science

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Caudal somites are generated from a pool of progenitor cells located in the tailbud region. These progenitor cells form the presomitic mesoderm that gradually differentiates into somites under the action of the segmentation clock. The signals responsible for tailbud mesoderm progenitor pool maintenance during axial elongation are still elusive. Here, we show that Bmp signaling is sufficient to activate the entire mesoderm progenitor gene signature in primary cultures of caudal mesoderm cells. Bmp signaling acts through the key regulatory genes brachyury (T ) and Nkx1-2 and contributes to the activation of several other regulators of the mesoderm progenitor gene network. In the absence of Bmp signaling, tailbud mesoderm progenitor cells acquire aberrant gene expression signatures of the heart, blood, muscle and skeletal embryonic lineages. Treatment of embryos with the Bmp inhibitor noggin confirmed the requirement for Bmp signaling for normal T expression and the prevention of abnormal lineage marker activation. Together, these results identify Bmp signaling as a non-cell-autonomous signal necessary for mesoderm progenitor cell homeostasis.

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Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development

Cited by 141 publications

References 52 publications

A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border

A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border

Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish

Bmp signaling maintains a mesoderm progenitor cell state in the mouse tailbud

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