Transglutaminase 2 expressed in mast cells recruited into skin or bone marrow induces the development of pediatric mastocytosis

Ahn, Young Min; Hong, Gwan Ui; Kim, Sang Hoon; Lee, Ho Jung; Baek, Heysung; Kim, Myung Nam; Park, Kui Young; Ro, Jai Youl

doi:10.1111/pai.12403

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…After washing the membranes with PBST, they were treated with primary Abs against actin, MMP13, TNF-α, IL-1β, COX-2, or P65 (Cell Signaling Technology, Bevery, MA, USA), or collagen type II (Santa Cruz, CA, USA) diluted with PBST (1:1000). Membranes were washed with PBST and treated with horseradish peroxidase (HRP)-conjugated goat anti-mouse or HRP-conjugated goat anti-rabbit IgG (diluted to 1:5000~1:10,000) (Bethyl Laboratories, Montgomery, TX, USA) in PBST for 1 h. After washing, the protein bands were visualized by Enhanced Chemi-Luminescence using a chemiluminometer (ECL; Amersham Biosciences, Piscataway, NJ, USA) [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

et al. 2018

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Osteoarthritis (OA) is the common form of arthritis and is characterized by disability and cartilage degradation. Although natural product extracts have been reported to have anti-osteoarthritic effects, the potential bioactivity of Ryupunghwan (RPH), a traditional Korean medicinal botanical formula that contains Astragalus membranaceus, Turnera diffusa, Achyranthes bidentata, Angelica gigas, Eclipta prostrata, Eucommia ulmoides, and Ilex paraguariensis, is not known well. Therefore, the inhibitory effects of single compounds isolated from RPH on the OA-related molecules were investigated using IL-1β-stimulated chondrosarcoma SW1353 (SW1353) cell model. Two bioactive compounds, isomucronulatol 7-O-β-d-glucoside (IMG) and ecliptasaponin A (ES) were isolated and purified from RPH using column chromatography, and then the structures were analyzed using ESI-MS, 1H-NMR, and 13C-NMR spectrum. The expression or amount of matrix metalloproteinase 13 (MMP13), COX1/2, TNF-α, IL-1β or p65 was determined by RT-PCR, Western blot, and enzyme-linked immunosorbent assay (ELISA). RPH pretreatment reduced the expression and amounts of MMP13, and the expression of collagen II, COX1/2, TNF-α, IL-1β or p65, which were increased in IL-1β-stimulated SW1353 cells. IMG reduced the expression of all OA-related molecules, but the observed inhibitory effect was less than that of RPH extract. The other single compound ES showed the reduced expression of all OA-related molecules, and the effect was stronger than that in IMG (approximately 100 fold). Combination pretreatment of both single components remarkably reduced the expression of MMP13, compared to each single component. These synergic effects may provide potential molecular modes of action for the anti-osteoarthritic effects of RPH observed in clinical and animal studies.

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Section: Methodsmentioning

confidence: 99%

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

et al. 2018

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“…MC-derived OSM stimulates the growth of microvascular endothelial cells, osteoblasts, and fibroblasts [ 139 ]. Furthermore, it was shown that in children with mastocytosis, MCs may migrate to the skin and BM as a result of the upregulation of CCL2/chemokine receptor 2 (CCR2) and vascular cell adhesion molecule (VCAM-1), and they may induce the expression/activation of transglutaminase 2 (TG2) [ 144 ]. Importantly, TG2 is a cross-linking enzyme that promotes the expression of inflammatory cytokines, histamine, and LTC4 [ 144 ].…”

Section: Cutaneous Mastocytosismentioning

confidence: 99%

“…Furthermore, it was shown that in children with mastocytosis, MCs may migrate to the skin and BM as a result of the upregulation of CCL2/chemokine receptor 2 (CCR2) and vascular cell adhesion molecule (VCAM-1), and they may induce the expression/activation of transglutaminase 2 (TG2) [ 144 ]. Importantly, TG2 is a cross-linking enzyme that promotes the expression of inflammatory cytokines, histamine, and LTC4 [ 144 ]. An assessment of the precise role of all MC mediators in the pathogenesis of mastocytosis requires further studies.…”

Section: Cutaneous Mastocytosismentioning

confidence: 99%

The Role of Mast Cells in the Induction and Maintenance of Inflammation in Selected Skin Diseases

Woźniak

Owczarczyk‐Saczonek

Lange

et al. 2023

IJMS

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Under physiological conditions, skin mast cells play an important role as guardians that quickly react to stimuli that disturb homeostasis. These cells efficiently support, fight infection, and heal the injured tissue. The substances secreted by mast cells allow for communication inside the body, including the immune, nervous, and blood systems. Pathologically non-cancerous mast cells participate in allergic processes but also may promote the development of autoinflammatory or neoplastic disease. In this article, we review the current literature regarding the role of mast cells in autoinflammatory, allergic, neoplastic skin disease, as well as the importance of these cells in systemic diseases with a pronounced course with skin symptoms.

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Association of TG2 from mast cells and chronic spontaneous urticaria pathogenesis

Hong

Bae

et al. 2016

Annals of Allergy, Asthma & Immunology

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Transglutaminase 2 expressed in mast cells recruited into skin or bone marrow induces the development of pediatric mastocytosis

Cited by 4 publications

References 31 publications

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

The Role of Mast Cells in the Induction and Maintenance of Inflammation in Selected Skin Diseases

Association of TG2 from mast cells and chronic spontaneous urticaria pathogenesis

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