Transglutaminase C in cerebellar granule neurons: Regulation and localization of substrate cross-linking

Perry, Michael J.; Mahoney, Sally-Ann; Haynes, Laurence W.

doi:10.1016/0306-4522(94)00556-k

Cited by 53 publications

(36 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The latter enzymes play a number of roles in many different tissues, including axonal growth (15), differentiation (16), and cell death (30 -32). They may participate in the Ca 2ϩ -dependent crosslinking of cell membranes and cytoskeletal proteins (28).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of GTP-dependent and GTP-independent Tissue-type Transglutaminase in Cytokine-treated Rat Brain Astrocytes

Monsonego

Shani

Friedmann

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

Tissue-type transglutaminases (TGases) were recently shown to exert dual enzymatic activities; they catalyze the posttranslational modification of proteins by transamidation, and they also act as guanosine triphosphatase (GTPase). Here we show that a tissue-type TGase is expressed in rat brain astrocytes in vitro, and is induced by the inflammation-associated cytokines interleukin-1␤ and to a lesser extent by tumor necrosis factor-␣. Induction is accompanied by overexpression and appearance of an additional shorter clone, which does not contain the long 3 -untranslated region and encodes for a novel TGase enzyme whose C terminus lacks a site that affects the enzyme's interaction with guanosine triphosphate (GTP). Expression of two clones revealed that the long form is inhibited noncompetitively by GTP, but the short form significantly less so. The different affinities for GTP may account for the difference in physiological function between these two enzymes.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In developing rat cerebellar tissue-type TGase, immunoreactivity is detectable in axons of the external granular layer during the early postnatal period (14,15). In PC12 cells, induction of neurite formation is accompanied by an elevation in TGase activity (16).…”

mentioning

confidence: 99%

Expression of GTP-dependent and GTP-independent Tissue-type Transglutaminase in Cytokine-treated Rat Brain Astrocytes

Monsonego

Shani

Friedmann

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The medium was then removed, monolayer gently washed with PBS and slides incubated for 6 h with 1 mM biotin-X-cadaverine (a cell permeable TG2 substrate; Perry et al, 1995) in serum-free DMEM experimentation. Where appropriate, cells were treated for 1 h with Table overlay provided by the software.…”

Section: Visualisation Of In Situ Tg2 Activity and Neuronal Markersmentioning

confidence: 99%

Role of transglutaminase 2 in PAC1 receptor mediated protection against hypoxia-induced cell death and neurite outgrowth in differentiating N2a neuroblastoma cells

Algarni

Hargreaves

Dickenson

2017

Biochemical Pharmacology

View full text Add to dashboard Cite

“…The cells were then incubated for 6 h in medium containing 1 mM biotin-X-cadaverine (a cell permeable TG2 substrate; [30] Images were collected every 1.7 s for 10 min. Increases in intracellular Ca 2+ were defined as F/F0 where F was the fluorescence at any given time, and F0 was the initial basal level of Ca 2+ .…”

Section: Measurement Of In Situ Tg2 Activitymentioning

confidence: 99%

A1 adenosine receptor-induced phosphorylation and modulation of transglutaminase 2 activity in H9c2 cells: A role in cell survival

Vyas

Hargreaves

Bonner

et al. 2016

Biochemical Pharmacology

View full text Add to dashboard Cite

The regulation of tissue transglutaminase (TG2) activity by the GPCR family is poorly understood. In this study, we investigated the modulation of TG2 activity by the A1 adenosine receptor in cardiomyocyte-like H9c2 cells.H9c2 cells were lysed following stimulation with the A1 adenosine receptor agonist N 6 -cyclopentyladenosine (CPA).Transglutaminase activity was determined using an amine incorporating and a protein cross linking assay. TG2 phosphorylation was assessed via immunoprecipitation and 3

show abstract

Transglutaminase C in cerebellar granule neurons: Regulation and localization of substrate cross-linking

Cited by 53 publications

References 57 publications

Expression of GTP-dependent and GTP-independent Tissue-type Transglutaminase in Cytokine-treated Rat Brain Astrocytes

Expression of GTP-dependent and GTP-independent Tissue-type Transglutaminase in Cytokine-treated Rat Brain Astrocytes

Role of transglutaminase 2 in PAC1 receptor mediated protection against hypoxia-induced cell death and neurite outgrowth in differentiating N2a neuroblastoma cells

A1 adenosine receptor-induced phosphorylation and modulation of transglutaminase 2 activity in H9c2 cells: A role in cell survival

Contact Info

Product

Resources

About