Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants

At the last stage of the blood coagulation cascade, thrombin plays a central role in the processing of fibrinogen for the polymerization and in the additional activation of Factor XIII for the stable cross-linking of fibrin. In addition, thrombin carries out possible multiple roles via processing or interaction with various functional proteins. Several studies conducted in order to elucidate additional physiological significance are ongoing. To clarify further significance of thrombin and to establish an associated disease model, we characterized the orthologue gene for medaka (Oryzias latipes), a research model fish. Tissue distribution of medaka prothrombin has been immunotechnically analyzed. Furthermore, thrombin-deficient medaka mutants were viably established by utilizing a genome-editing method. The established gene-deficient mutants exhibited retarded blood coagulation even in the heterozygous fish. Taking advantage of their ease of handling, this specific model is useful for further investigation in medical research areas on human coagulation diseases.

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Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants

Cited by 7 publications

References 31 publications

Editorial for the special issue on transglutaminases in translation – Novel tools and methods impacting on diagnostics and therapeutics

Editorial for the special issue on transglutaminases in translation – Novel tools and methods impacting on diagnostics and therapeutics

Enzymatic activity changes in striped catfish Pseudoplatystoma magdaleniatum, induced by exposure to different concentrations of ibuprofen and triclosan

Thrombin-deficient mutant of medaka, a model fish, displays serious retardation in blood coagulation

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